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FluxMPS™ CMRL1066 Medium w/o L-Glutamine, Phenol Red: 1X Liquid

Product#: DCP-CMRL-QR1X
$64.90
DCP-CMRL-QR1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
ISO 13485 Certified Manufacturing

FluxMPS™ CMRL1066 Medium w/o L-Glutamine, Phenol Red: 1X Liquid

Contains Sodium Bicarbonate Contains Calcium Contains Magnesium Contains Glucose Without L-Glutamine Without Phenol Red Without HEPES Without Sodium Pyruvate

FluxMPS™ CMRL1066 Medium w/o L-Glutamine, Phenol Red: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • Quadruple-stage filtration: 0.1 µm (Prefiltration I & II) + 0.04 µm (Final filtration I & II), reaching a 0.04 µm final pore size
  • Endotoxin release specification: < 0.05 EU/mL (USP <85> BET)
  • CMRL 1066 base formulation without L-glutamine and without phenol red; pH 7.4 (USP <791>)
  • Contains 1000 mg/L D-glucose and 2200 mg/L sodium bicarbonate as the primary buffer system
  • Manufactured under an ISO 13485:2016 quality management system with ISO Class 5 aseptic fill & finish; final QC in Totowa, NJ
  • Mycoplasma risk mitigated via 0.1 µm mycoplasma-retentive filtration (not tested per lot)
  • Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request
DCP-CMRL-QR1X | Cell Culture Media Sizes: 500 mL, 1000 mL UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
CMRL1066 Medium w/o L-Glutamine, Phenol Red: 1X Liquid
  • Glucose1000 mg/L (1.0 g/L)
  • L-GlutamineNot present — supplement as needed
  • Sodium PyruvateNot present
  • HEPESNot present
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)Contact for specification
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and endotoxin variability that accumulate inside microchannels — corrupting biosensor readings and shortening device lifetimes. FluxMPS™ addresses these failure modes with a validated four-stage filtration train.

filter_alt

Microchannel-safe purity

0.04 µm final filter stage retains particles down to sub-mycoplasma size; USP <788> particulate compliance verified per lot.

target

Total metabolic control

User-defined carbon source and precise nutrient concentrations support metabolic flux experiments and glucose-titration studies.

water_drop

Ultrapure-grade water

Prepared with Type 1 water (18.2 MΩ·cm) with tightly controlled trace-metal and total organic carbon levels for reproducible, low-background cell culture performance.

visibility

Low background for imaging

Ultra-low particulate baseline from Quadruple-stage filtration supports confocal microscopy, live-cell biosensors, and TEER measurements without particulate interference.

science

Rich, stable nutrient profile

Micro-batch precision manufacturing locks in amino acid and vitamin concentrations, ensuring lot-to-lot reproducibility critical for long-term perfusion studies.

tune

Customization on demand

pH, glucose concentration, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ CMRL1066 Medium w/o L-Glutamine, Phenol Red: 1X Liquid is processed through a four-stage serial filtration sequence reaching a 0.04 µm final pore size — addressing mycoplasma-sized particulates, subvisible particles, and bioburden that 0.22 µm filtration cannot address. The train runs as two paired stages, each 0.04 µm final filter protected by its own dedicated 0.1 µm prefilter.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris and protein aggregates; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains mycoplasma-sized (0.2–0.3 µm) and sub-micron particulates that pass a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter protecting the second 0.04 µm final filter cartridge; guards against breakthrough from Stage 1.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill performed in a validated ISO Class 5 laminar-flow workstation.

Performance vs. conventional media

By reaching a 0.04 µm final pore size across four sequential stages, FluxMPS™ delivers approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration, with full USP <788> compliance verified on every production lot.

5×
 
0.04
µm final filter pore size — sub-mycoplasma polishing
Sterility assurance: Each production lot undergoes 14-day USP <71> sterility testing and must pass before release. Mycoplasma risk is mitigated through 0.1 µm mycoplasma-retentive filtration (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ CMRL1066 Medium w/o L-Glutamine, Phenol Red: 1X Liquid (DCP-CMRL-QR1X) Quadruple-stage filtration system diagram showing four sequential stages: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, and 0.04 micron Final filtration II for organ-on-a-chip and microfluidic cell culture applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2) delivering sub-mycoplasma purity for MPS and OoC applications.
© Diagnocine® — DCP-CMRL-QR1X
Applications

Designed for next-generation cell culture platforms

FluxMPS™ CMRL1066 Medium w/o L-Glutamine, Phenol Red: 1X Liquid is validated for use across organ-on-a-chip, metabolic research, live-cell imaging, and primary cell models where particulate contamination and endotoxin variation are unacceptable.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade ultra nano-filtered variant is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion.

  • Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
  • Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
  • Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs

Inquiry Required: The 0.01 µm MPS Grade variant is available by special order. Contact support@diagnocine.com to request this variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-filtered formulation prevents microchannel clogging and maintains laminar flow integrity across complex chip geometries.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Defined carbon source and low-endotoxin background enables precise metabolic flux analysis and Warburg effect studies.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Ultrapure formulation supports sensitive iPSC differentiation protocols where endotoxin and particulates cause off-target effects.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Microchannel-safe purity essential for maintaining endothelial barrier integrity and TEER values in perfusion models.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Chemically defined amino acid and vitamin profile supports isotope tracing and mass-spectrometry-based metabolomics workflows. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

13C tracingNMR metabolomicsLC-MS
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate baseline supports high-content confocal imaging and optical biosensor integration.

ConfocalBiosensorsTEER
Technical Specifications

Lot-release quality parameters

Every production lot of FluxMPS™ CMRL1066 Medium w/o L-Glutamine, Phenol Red: 1X Liquid undergoes the complete quality-release battery listed below before shipment.

Physical & Chemical Parameters
Parameter Specification
Formulation CMRL1066 Medium; contains Sodium Bicarbonate, Calcium, Magnesium, Glucose; without L-Glutamine, Phenol Red, HEPES, Sodium Pyruvate
Appearance Pale-yellow to colorless, clear solution
pH USP <791> 7.4
Osmolality USP <785> Contact for specification
Glucose 1000 mg/L (1.0 g/L)
L-Glutamine Not present — supplement as needed
Sodium Pyruvate Not present
Phenol Red Not present
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification)
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Compliant
Particulate ≥25 µm USP <788> Compliant
Water Purity Ultrapure Type 1, 18.2 MΩ·cm (ASTM D1193 Type I)
Manufacturing std. ISO 13485:2016 ISO 13485
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protected from light
Freeze-thaw Not recommended
Shelf life 12 months from date of manufacture, unopened
Shipping condition Ambient or cold pack; on ice for extended transit
CO2 requirement 5-6% CO2 (derived from 2,200 mg/L sodium bicarbonate to maintain pH 7.4)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Cell culture / reagent grade
Traceability Full lot documentation, CoA available
Manufacturing QMS ISO 13485:2016 certified
Grade Microfluidics Suitable (0.04 µm final filtration)
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch precision manufacturing
Intended use For Research Use Only (RUO)
Formulation

Full composition (mg/L)

Complete formulation with CAS numbers. All ingredient names and mg/L values are reproduced from the manufacturer specification. Custom compositions available on request.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.690
Potassium chloride 7447-40-7 400.000
Sodium acetate anhydrous 127-09-3 50.000
Sodium bicarbonate 144-55-8 2200.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 50.000
L-Alanine 56-41-7 25.000
L-Arginine 74-23-7 57.870
L-Aspartic acid 56-84-8 30.000
L-Cysteine hydrochloride monohydrate 7048-04-6 260.000
L-Cystine dihydrochloride 30189-89-0 20.000
L-Glutamic acid 56-86-0 75.000
L-Histidine hydrochloride monohydrate 5934-29-2 20.000
L-Isoleucine 73-32-5 20.000
L-Leucine 61-90-5 60.000
L-Lysine hydrochloride 657-27-2 70.000
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 25.000
L-Proline 147-85-3 40.000
L-Serine 56-45-1 25.000
L-Threonine 72-19-5 30.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-45-6 40.000
L-Valine 72-18-4 25.000
Trans-4-Hydroxy-L-Proline 51-35-4 10.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 0.500
D-Biotin 58-85-5 0.010
D-Pantothenate (Hemicalcium) 137-08-6 0.010
Folic acid 59-30-3 0.010
L-Ascorbic acid sodium salt 134-03-2 50.000
Nicotinamide 98-92-0 0.025
Nicotinic acid 59-67-6 0.025
Pyridoxal hydrochloride 65-22-5 0.025
Pyridoxine hydrochloride 58-56-0 0.025
Riboflavin 83-88-5 0.010
Thiamine hydrochloride 67-03-8 0.010
myo-Inositol 87-89-8 0.050
p-Amino benzoic acid (PABA) 150-13-0 0.050
OTHERS
2' Deoxyadenosine 958-09-8 10.000
2' Deoxycytidine hydrochloride 2' 960-71-4 10.000
Deoxyguanosine 961-07-9 10.000
5-Methyldeoxycytidine 838-07-3 0.100
Cholesterol 57-88-5 0.200
Cocarboxylase 154-87-0 1.000
Coenzyme A sodium salt 102029-73-2 2.500
D-Glucose 50-99-7 1000.000
D-Glucuronic acid sodium salt 207569-96-4 3.880
FAD disodium salt 84366-81-4 0.106
Glutathione reduced 70-18-8 10.000
Thymidine 50-89-5 10.000
Tween 80 9005-65-6 5.000
Uridine-5-Triphosphate.Na 19817-92-6 1.000
β-NAD 53-84-9 7.000
β-NADP sodium salt 1184-16-3 1.000
Customization: pH, glucose concentration, salt balance, HEPES concentration, and full nutrient profile are available on request. Contact support@diagnocine.com with your specifications.
Quality Assurance

ISO 13485:2016 manufacturing & compliance

FluxMPS™ CMRL1066 Medium w/o L-Glutamine, Phenol Red: 1X Liquid is manufactured under a full ISO 13485:2016 quality management system, with final packaging, testing, and customization completed at Diagnocine Precision in Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering all manufacturing, testing, and release processes for every production lot.

water_drop

Ultrapure Type 1 Water

All media prepared with 18.2 MΩ·cm resistivity water with controlled trace-metal and total organic carbon levels.

biotech

ISO Class 5 Fill & Finish

Aseptic filling performed in validated laminar-flow (ISO Class 5 / Class 100) workstations; 21 CFR Part 820 (QMSR) aligned.

assignment

Micro-Batch Precision

Small-batch manufacturing locks in lot-to-lot nutrient consistency critical for reproducible perfusion studies and long-term OoC experiments.

Endotoxin — USP <85> BET

LAL assay performed on every batch; assay sensitivity 0.005 EU/mL. Release specification: < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration particle count test confirms ≥10 µm and ≥25 µm particulate compliance on every lot.

Osmolality — USP <785>

Freezing-point osmometry performed per USP <785>. Result: Contact for specification.

Documentation — CoA & Full Lot Records

Certificate of Analysis available for every lot, including full QC panel, raw material traceability, and release signatures.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-CMRL-QR1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered alternatives of the same base formulation.

Parameter DCP-CMRL-QR1X (FluxMPS™) Conventional CMRL1066 (0.22 µm) Standard CMRL1066 alternative
Grade Microfluidics Suitable Standard grade (0.22 µm) Standard grade (0.22 µm)
Base Formulation CMRL1066 w/o L-Glutamine, Phenol Red CMRL1066 Standard CMRL1066 Equivalent
Phenol red-free & L-glutamine-free formulation check_circle cancel cancel
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 stages 1 stage 1–2 stages
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle USP <788> cancel cancel
Water quality Ultrapure Type 1 (18.2 MΩ) Purified water Purified water
Manufacturing QMS ISO 13485:2016 Variable Variable
Microfluidic channel compatibility check_circle Validated cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle On request cancel Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ CMRL1066 Medium w/o L-Glutamine, Phenol Red: 1X Liquid and Microfluidics Suitable cell culture media.

Yes. DCP-CMRL-QR1X is processed through our Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), yielding ultra-low particulate counts that help prevent microchannel clogging in OoC and MPS devices.
Standard 0.22 µm filtration leaves intact mycoplasma-sized particulates (0.2–0.3 µm diameter) and substantial subvisible particulates that can accumulate inside microchannels. FluxMPS™ uses four sequential stages reaching 0.04 µm, addressing these contaminants and delivering approximately 5× lower particulate counts per USP <788>.
This formulation is manufactured without L-glutamine (which degrades in liquid storage and is typically added fresh at time of use) and without phenol red (to eliminate pH-indicator interference in fluorescence, absorbance, and optical biosensor assays). Add L-glutamine or a stabilized dipeptide substitute immediately before use per your cell line's requirements. Contact support@diagnocine.com for a custom formulation.
This formulation contains 2,200 mg/L sodium bicarbonate as its primary buffer. Based on the bicarbonate concentration, an atmosphere of approximately 5-6% CO2 is required to maintain the target pH of 7.4; validate empirically for your specific incubator and cell type.
Yes. FluxMPS™ CMRL1066 Medium w/o L-Glutamine, Phenol Red: 1X Liquid can be supplemented with FBS (typically 5–20%), growth factors, antibiotics, or L-glutamine per standard cell culture practice. Add supplements immediately before use. When post-filtering serum or protein-containing additions, use a 0.2 µm low-protein-binding PES or PVDF membrane — never a 0.04 µm membrane, which will retain serum proteins and lipoproteins and clog rapidly.
Endotoxin is controlled per manufacturing batch. Every batch is tested by LAL assay per USP <85> BET (assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before shipment. Batch-specific results are documented in the Certificate of Analysis available on request from support@diagnocine.com.
Yes. A lot-specific CoA is available for every shipment and includes: appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility (USP <71>), particulate matter (USP <788>), raw material traceability, manufacturing date, and authorized release signatures. Request via support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed publications supporting the scientific rationale for Microfluidics Suitable, ultra-filtered media and microfluidic cell culture applications.

  1. Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
  3. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
  4. Warburg O (1956). On the origin of cancer cells. Science, 123(3191), 309–314. doi:10.1126/science.123.3191.309
  5. Emmons EV (1965). Detection of mycoplasma in cell cultures using filtration. Proceedings of the Society for Experimental Biology, 118, 1010–1015. doi:10.3181/00379727-118-29988
  6. Kim S et al. (2012). Gut-on-a-chip microdevice replicates key functional features of the human intestine. Lab on a Chip, 12(12), 2165–2174. doi:10.1039/c2lc40074j
  7. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
  8. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
  9. Schuster B et al. (2020). Automated microfluidic platform for dynamic and combinatorial drug screening of tumor organoids. Nature Communications, 11, 5271. doi:10.1038/s41467-020-19058-4
  10. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175

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