FluxMPS™ CMRL 1066 Medium w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid
FluxMPS™ CMRL 1066 Medium w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid
FluxMPS™ CMRL 1066 Medium w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.
- Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size for microchannel-safe purity
- Endotoxin release specification: less than 0.05 EU/mL (USP <85> BET)
- CMRL 1066 base formulation supplied without L-Glutamine and without Sodium Bicarbonate; pH 7.4 (USP <791>) — both must be supplemented before use
- Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) under controlled trace-metal and TOC conditions
- Manufactured under an ISO 13485:2016 quality management system; aseptic ISO Class 5 fill and finish
- Contains phenol red, calcium, magnesium, and 1000 mg/L D-glucose; formulated without HEPES or sodium pyruvate
- Custom pH, glucose concentration, salts, HEPES, and nutrient composition available on request — contact support@diagnocine.com
- Glucose1000 mg/L (1.0 g/L)
- L-GlutamineNot present — supplement as needed
- Sodium PyruvateNot present
- HEPESNot present
- pH (USP <791>)7.4
- Osmolality (USP <785>)Contact for specification
- Endotoxin (USP <85>)less than 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2
- Storage2–8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and endotoxin spikes that accumulate inside microchannels — corrupting biosensor readings, triggering inflammation in primary cultures, and shortening device lifetimes. FluxMPS™ is engineered to address these failure modes at the filtration stage.
Microchannel-safe purity
0.04 µm final filter stage retains particles down to sub-mycoplasma size; USP <788> Method 1 (light obscuration) particulate compliance verified per lot.
Total metabolic control
User-defined carbon source and precise nutrient concentrations enable custom metabolic flux experiments — glucose-free variants available on request.
Ultrapure-grade water
Prepared with Type 1 water (18.2 MΩ·cm) meeting ASTM D1193 / ISO 3696 purity criteria, with tight control of trace metals and total organic carbon (TOC).
Low background for imaging
Ultra-low particulate baseline supports confocal microscopy, live-cell biosensors, and TEER measurements.
Rich, stable nutrient profile
Micro-batch precision manufacturing locks in amino acid and vitamin concentrations, ensuring lot-to-lot reproducibility critical for long-term perfusion studies.
Customization on demand
pH, glucose concentration, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
FluxMPS™ CMRL 1066 Medium w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid is processed through a four-stage serial filtration sequence that reaches 0.04 µm — addressing mycoplasma-scale and subvisible particulate contamination that 0.22 µm filtration cannot resolve.
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1
0.1 µm Prefiltration I
Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm final filter cartridge.
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2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass through a standard 0.22 µm filter.
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3
0.1 µm Prefiltration II
Second dedicated prefilter protecting the second 0.04 µm cartridge; provides full redundancy across the train.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill and finish performed in a validated ISO Class 5 laminar-flow workstation.
Performance vs. conventional media
By reaching a 0.04 µm final pore size across four sequential stages, FluxMPS™ delivers approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration, with USP <788> Method 1 compliance verified on every production lot.
© Diagnocine® — DCP-CMRL-QB1X
Designed for next-generation cell culture platforms
FluxMPS™ CMRL 1066 Medium w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid is validated for use across organ-on-a-chip, metabolic research, live-cell imaging, and primary cell models where particulate contamination and endotoxin variation are unacceptable.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) MPS Grade variant is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion.
- Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
- Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
- Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs
Inquiry Required: The 0.01 µm MPS Grade variant is available by special order. Contact support@diagnocine.com to request this variant.
Micro Physiological System (MPS) & Chip
Ultra-filtered formulation prevents microchannel clogging and maintains laminar flow integrity across complex chip geometries.
Warburg Effect & Metabolic Research
Defined carbon source and low-endotoxin background support precise metabolic flux analysis and Warburg effect studies.
iPSC-Derived Models
Ultrapure formulation supports sensitive iPSC differentiation protocols where endotoxin and particulates cause off-target effects.
Endothelial & Primary Cells
Microchannel-safe purity supports endothelial barrier integrity and TEER values in perfusion models.
Metabolic Flux Analysis
Chemically defined base with controlled nutrient composition supports isotope tracing (13C) and NMR metabolomics workflows. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Ultra-low particulate load ideal for high-content confocal imaging and optical biosensor integration.
Lot-release quality parameters
Every production lot of FluxMPS™ CMRL 1066 Medium w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid undergoes the complete quality-release battery listed below before shipment.
| Parameter | Specification |
|---|---|
| Formulation | CMRL 1066 Medium w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid (contains Phenol Red, Calcium, Magnesium, Glucose; without L-Glutamine, Sodium Bicarbonate, HEPES, Sodium Pyruvate) |
| Appearance | Orange-red colored, clear solution |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | Contact for specification |
| Glucose | 1000 mg/L (1.0 g/L) |
| L-Glutamine | Not present — supplement as needed |
| Sodium Pyruvate | Not present |
| Phenol Red | Present (21.240 mg/L) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | less than 0.05 EU/mL |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | Compliant |
| Particulate ≥25 µm USP <788> Method 1 | Compliant |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 ISO 13485 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, protected from light |
| Freeze-thaw | Not recommended |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO2 requirement | Supplied without sodium bicarbonate and without HEPES; add sodium bicarbonate (typical 2.2 g/L) for 5% CO2 incubation, or supplement with HEPES for ambient buffering — contact support@diagnocine.com for guidance. |
| Parameter | Specification |
|---|---|
| Raw material grade | Cell culture / reagent grade |
| Traceability | Full lot documentation, CoA available |
| Manufacturing QMS | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch precision manufacturing |
| Intended use | For Research Use Only (RUO) |
Full composition (mg/L)
Complete formulation with CAS numbers, reproduced in full from the manufacturer specification. Custom compositions available on request.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.690 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium acetate anhydrous | 127-09-3 | 50.000 |
| Sodium chloride | 7647-14-5 | 6800.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 122.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 50.000 |
| L-Alanine | 56-41-7 | 25.000 |
| L-Arginine | 74-23-7 | 57.870 |
| L-Aspartic acid | 56-84-8 | 30.000 |
| L-Cysteine hydrochloride monohydrate | 7048-04-6 | 260.000 |
| L-Cystine dihydrochloride | 30189-89-0 | 20.000 |
| L-Glutamic acid | 56-86-0 | 75.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 20.000 |
| L-Isoleucine | 73-32-5 | 20.000 |
| L-Leucine | 61-90-5 | 60.000 |
| L-Lysine hydrochloride | 657-27-2 | 70.000 |
| L-Methionine | 63-68-3 | 15.000 |
| L-Phenylalanine | 63-91-2 | 25.000 |
| L-Proline | 147-85-3 | 40.000 |
| L-Serine | 56-45-1 | 25.000 |
| L-Threonine | 72-19-5 | 30.000 |
| L-Tryptophan | 73-22-3 | 10.000 |
| L-Tyrosine disodium salt dihydrate | 69847-45-6 | 40.000 |
| L-Valine | 72-18-4 | 25.000 |
| Trans-4-Hydroxy-L-Proline | 51-35-4 | 10.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 0.500 |
| D-Biotin | 58-85-5 | 0.010 |
| D-Pantothenate (Hemicalcium) | 137-08-6 | 0.010 |
| Folic acid | 59-30-3 | 0.010 |
| L-Ascorbic acid sodium salt | 134-03-2 | 50.000 |
| Nicotinamide | 98-92-0 | 0.025 |
| Nicotinic acid | 59-67-6 | 0.025 |
| Pyridoxal hydrochloride | 65-22-5 | 0.025 |
| Pyridoxine hydrochloride | 58-56-0 | 0.025 |
| Riboflavin | 83-88-5 | 0.010 |
| Thiamine hydrochloride | 67-03-8 | 0.010 |
| myo-Inositol | 87-89-8 | 0.050 |
| p-Amino benzoic acid (PABA) | 150-13-0 | 0.050 |
| OTHERS | ||
| 2' Deoxyadenosine | 958-09-8 | 10.000 |
| 2' Deoxycytidine hydrochloride 2' | 960-71-4 | 10.000 |
| Deoxyguanosine | 961-07-9 | 10.000 |
| 5-Methyldeoxycytidine | 838-07-3 | 0.100 |
| Cholesterol | 57-88-5 | 0.200 |
| Cocarboxylase | 154-87-0 | 1.000 |
| Coenzyme A sodium salt | 102029-73-2 | 2.500 |
| D-Glucose | 50-99-7 | 1000.000 |
| D-Glucuronic acid sodium salt | 207569-96-4 | 3.880 |
| FAD disodium salt | 84366-81-4 | 0.106 |
| Glutathione reduced | 70-18-8 | 10.000 |
| Phenol red sodium salt | 34487-61-1 | 21.240 |
| Thymidine | 50-89-5 | 10.000 |
| Tween 80 | 9005-65-6 | 5.000 |
| Uridine-5-Triphosphate.Na | 19817-92-6 | 1.000 |
| β-NAD | 53-84-9 | 7.000 |
| β-NADP sodium salt | 1184-16-3 | 1.000 |
ISO 13485:2016 manufacturing & compliance
FluxMPS™ CMRL 1066 Medium w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid is manufactured under a full ISO 13485:2016 quality management system, with final packaging, testing, and customization completed at Diagnocine Precision in Totowa, New Jersey, USA.
ISO 13485:2016 QMS
Full quality management system certification covering all manufacturing, testing, and release processes for every production lot.
Ultrapure Type 1 Water
All media prepared with 18.2 MΩ·cm resistivity Type 1 water (ASTM D1193 / ISO 3696), with controlled trace-metal and TOC content.
ISO Class 5 Fill & Finish
Aseptic filling performed in validated laminar-flow (ISO Class 5 / Class 100) workstations; 21 CFR Part 820 (QMSR) aligned.
Micro-Batch Precision
Small-batch manufacturing locks in lot-to-lot nutrient consistency critical for reproducible perfusion studies and long-term OoC experiments.
Endotoxin — USP <85> BET
Limulus Amebocyte Lysate (LAL) assay performed on every manufacturing batch. Release specification: less than 0.05 EU/mL.
Particulate — USP <788> Method 1
Light obscuration particle count test confirms ≥10 µm and ≥25 µm particulate compliance on every lot.
Osmolality — USP <785>
Freezing-point osmometry performed per USP <785>. Result: Contact for specification.
Documentation — CoA & Full Lot Records
Certificate of Analysis available for every lot, including full QC panel, raw material traceability, and release signatures.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification less than 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-CMRL-QB1X (FluxMPS™) compares
Side-by-side comparison against conventional 0.22 µm-filtered alternatives of the same base formulation.
| Parameter | DCP-CMRL-QB1X (FluxMPS™) | Conventional CMRL 1066 (0.22 µm) | Standard CMRL 1066 alternative |
|---|---|---|---|
| Grade | Microfluidics Suitable | Standard grade | Standard grade |
| Base Formulation | CMRL 1066 w/o L-Glutamine, Sodium Bicarbonate | CMRL 1066 (complete, with L-Glutamine & Sodium Bicarbonate) | CMRL 1066 equivalent (complete) |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 stages | 1 stage | 1–2 stages |
| Mycoplasma barrier filtration | check_circle | cancel | cancel |
| Endotoxin (release specification) | FluxMPS™ — less than 0.05 EU/mL | Corning classical liquid media — less than 0.25 EU/mL Sigma-Aldrich DMEM complete medium — 2 EU/mL or less Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP particulate compliance | check_circle USP <788> Method 1 | cancel | cancel |
| Water quality | Ultrapure Type 1 (18.2 MΩ·cm) | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | Variable | Variable |
| Microfluidic channel compatibility | check_circle Validated | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle On request | cancel | Limited |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ CMRL 1066 Medium w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid and Microfluidics Suitable cell culture media.
Supporting literature
Peer-reviewed publications supporting the scientific rationale for ultra-filtered, Microfluidics Suitable media and microfluidic cell culture applications.
- Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
- Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
- Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
- Warburg O (1956). On the origin of cancer cells. Science, 123(3191), 309–314. doi:10.1126/science.123.3191.309
- Emmons EV (1965). Detection of mycoplasma in cell cultures using filtration. Proceedings of the Society for Experimental Biology, 118, 1010–1015. doi:10.3181/00379727-118-29988
- Kim S et al. (2012). Gut-on-a-chip microdevice replicates key functional features of the human intestine. Lab on a Chip, 12(12), 2165–2174. doi:10.1039/c2lc40074j
- Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
- Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
- Schuster B et al. (2020). Automated microfluidic platform for dynamic and combinatorial drug screening of tumor organoids. Nature Communications, 11, 5271. doi:10.1038/s41467-020-19058-4
- Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175
