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FluxMPS™ CMRL 1066 Medium w/o Sodium Bicarbonate: 1X Liquid

Product#: DCP-CMRL-B1X
$64.90
DCP-CMRL-B1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
ISO 13485 Certified Manufacturing

FluxMPS™ CMRL 1066 Medium w/o Sodium Bicarbonate: 1X Liquid

Contains L-Glutamine Contains Phenol Red Contains Calcium Contains Magnesium Contains Glucose Without Sodium Bicarbonate Without HEPES Without Sodium Pyruvate

FluxMPS™ CMRL 1066 Medium w/o Sodium Bicarbonate: 1X Liquid is a Microfluidics Suitable, ultra-filtered cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • Quadruple-stage nano-filtration reaching a 0.04 µm final pore size (0.1 µm ×2 + 0.04 µm ×2)
  • Endotoxin release specification: less than 0.05 EU/mL (USP <85> BET)
  • CMRL 1066 base formulation with 1000 mg/L (1.0 g/L) glucose and 100 mg/L L-glutamine
  • Formulated without sodium bicarbonate and without HEPES — buffering system to be added per your application
  • Contains phenol red (21.24 mg/L) as a pH indicator; orange-red colored, clear solution
  • Manufactured under an ISO 13485:2016 quality management system; final packaging and QC completed at Diagnocine, Totowa, NJ
  • Custom formulations available — pH, glucose, salts, and nutrient composition on request
DCP-CMRL-B1X | Cell Culture Media Size: 500 mL and 1000 mL UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
CMRL 1066 Medium w/o Sodium Bicarbonate: 1X Liquid
  • Glucose1000 mg/L (1.0 g/L)
  • L-Glutamine100 mg/L
  • Sodium PyruvateNot present
  • HEPESNot present
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)Contact for specification
  • Endotoxin (USP <85>)less than 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and variable endotoxin load that accumulate inside microchannels — corrupting biosensor readings and shortening device lifetimes. FluxMPS™ is built to reduce these failure modes at the source.

filter_alt

Microchannel-safe purity

0.04 µm final filter stage retains particles down to sub-mycoplasma size; USP <788> Method 1 (light obscuration) particulate compliance verified per lot.

target

Total metabolic control

Defined glucose and L-glutamine levels support precise nutrient-controlled experiments and metabolic flux studies.

water_drop

Ultrapure-grade water

Prepared with Type 1 water (18.2 MΩ·cm, ASTM D1193 / ISO 3696 Type I), with controlled trace-metal and total organic carbon (TOC) levels.

visibility

Low background for imaging

Quadruple-stage filtration reduces particulate baseline, supporting confocal microscopy, live-cell biosensors, and TEER measurements.

science

Rich, stable nutrient profile

Micro-batch precision manufacturing locks in amino acid and vitamin concentrations, supporting lot-to-lot reproducibility for long-term perfusion studies.

tune

Customization on demand

pH, glucose concentration, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ CMRL 1066 Medium w/o Sodium Bicarbonate: 1X Liquid is processed through a four-stage serial filtration sequence that reaches a 0.04 µm final pore size — addressing mycoplasma-sized particulates and subvisible particulate load beyond what single-pass 0.22 µm filtration can achieve.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates and aggregates; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I

    Fine particulate retention at a pore size below the typical mycoplasma diameter (0.2–0.3 µm).

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge for full-redundancy processing.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish performed in a validated ISO Class 5 (Class 100) laminar-flow workstation.

Performance vs. conventional media

By reaching a 0.04 µm final pore size across four sequential stages, FluxMPS™ delivers approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration, verified per USP <788> (light obscuration, Method 1) on every production lot.

5×
 
0.04
µm final filter pore size — sub-mycoplasma polishing
Sterility assurance: Every lot undergoes 14-day USP <71> sterility testing. Mycoplasma risk is mitigated via 0.1 µm mycoplasma-retentive filtration (not tested per lot). No bacterial or fungal growth observed in sterility testing.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS CMRL 1066 Medium w/o Sodium Bicarbonate 1X Liquid (DCP-CMRL-B1X) Quadruple-stage filtration system diagram showing four sequential stages: 0.1 micron Prefiltration I, 0.04 micron Final filtration I, 0.1 micron Prefiltration II, and 0.04 micron Final filtration II Polish for organ-on-a-chip and microfluidic cell culture applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2): Prefiltration I, Final filtration I, Prefiltration II, Final filtration II — Polish.
© Diagnocine® — DCP-CMRL-B1X
Applications

Designed for next-generation cell culture platforms

FluxMPS™ CMRL 1066 Medium w/o Sodium Bicarbonate: 1X Liquid is suited for use across organ-on-a-chip, metabolic research, live-cell imaging, and primary cell models where particulate contamination and endotoxin variation are unacceptable.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) MPS Grade variant is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion.

  • Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
  • Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
  • Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs

Inquiry Required: The 0.01 µm MPS Grade variant is available by special order. Contact support@diagnocine.com to request this variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-filtered formulation supports laminar flow integrity across complex chip geometries.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Defined glucose level and low-endotoxin release specification support metabolic flux analysis.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Ultrapure formulation supports sensitive iPSC differentiation protocols where endotoxin and particulates cause off-target effects.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Microchannel-safe purity supports endothelial barrier integrity and TEER measurements in perfusion models.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Defined base supports isotope tracing (13C, NMR) for metabolic flux analysis. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

13C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Low particulate load supports high-content confocal imaging and optical biosensor integration.

ConfocalBiosensorsTEER
Technical Specifications

Lot-release quality parameters

Every production lot of FluxMPS™ CMRL 1066 Medium w/o Sodium Bicarbonate: 1X Liquid undergoes the quality-release battery listed below before shipment.

Physical & Chemical Parameters
Parameter Specification
Formulation CMRL 1066 w/o Sodium Bicarbonate — contains Glucose, L-Glutamine, Calcium, Magnesium, Phenol Red; without Sodium Bicarbonate, HEPES, Sodium Pyruvate
Appearance Orange-Red colored, clear solution
pH USP <791> 7.4
Osmolality USP <785> Contact for specification
Glucose 1000 mg/L (1.0 g/L)
L-Glutamine 100 mg/L
Sodium Pyruvate Not present
Phenol Red Present (21.24 mg/L)
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET less than 0.05 EU/mL
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 Compliant
Particulate ≥25 µm USP <788> Method 1 Compliant
Water Purity Ultrapure Type 1, 18.2 MΩ·cm (ASTM D1193 / ISO 3696 Type I)
Manufacturing std. ISO 13485:2016 ISO 13485
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protected from light
Freeze-thaw Not recommended
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement Bicarbonate-free and HEPES-free as supplied; user must add a buffering system (sodium bicarbonate for CO2 incubator use, or HEPES for CO2-independent culture) based on the intended application
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Cell culture / reagent grade
Traceability Full lot documentation, CoA available
Manufacturing QMS ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch precision manufacturing
Intended use For Research Use Only (RUO)
Formulation

Full composition (mg/L)

Complete formulation with CAS numbers, reproduced from the manufacturer specification. Custom compositions available on request.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Magnesium sulfate anhydrous 7487-88-9 97.690
Potassium chloride 7447-40-7 400.000
Sodium acetate anhydrous 127-09-3 50.000
Sodium chloride 7647-14-5 6800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 122.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 50.000
L-Alanine 56-41-7 25.000
L-Arginine 74-23-7 57.870
L-Aspartic acid 56-84-8 30.000
L-Cysteine hydrochloride monohydrate 7048-04-6 260.000
L-Cystine dihydrochloride 30189-89-0 20.000
L-Glutamic acid 56-86-0 75.000
L-Glutamine 56-85-9 100.000
L-Histidine hydrochloride monohydrate 5934-29-2 20.000
L-Isoleucine 73-32-5 20.000
L-Leucine 61-90-5 60.000
L-Lysine hydrochloride 657-27-2 70.000
L-Methionine 63-68-3 15.000
L-Phenylalanine 63-91-2 25.000
L-Proline 147-85-3 40.000
L-Serine 56-45-1 25.000
L-Threonine 72-19-5 30.000
L-Tryptophan 73-22-3 10.000
L-Tyrosine disodium salt dihydrate 69847-45-6 40.000
L-Valine 72-18-4 25.000
Trans-4-Hydroxy-L-Proline 51-35-4 10.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 0.500
D-Biotin 58-85-5 0.010
D-Pantothenate (Hemicalcium) 137-08-6 0.010
Folic acid 59-30-3 0.010
L-Ascorbic acid sodium salt 134-03-2 50.000
Nicotinamide 98-92-0 0.025
Nicotinic acid 59-67-6 0.025
Pyridoxal hydrochloride 65-22-5 0.025
Pyridoxine hydrochloride 58-56-0 0.025
Riboflavin 83-88-5 0.010
Thiamine hydrochloride 67-03-8 0.010
p-Amino benzoic acid (PABA) 150-13-0 0.050
OTHERS
2' Deoxyadenosine 958-09-8 10.000
2' Deoxycytidine hydrochloride 2' 960-71-4 10.000
Deoxyguanosine 961-07-9 10.000
5-Methyldeoxycytidine 838-07-3 0.100
Cholesterol 57-88-5 0.200
Cocarboxylase 154-87-0 1.000
Coenzyme A sodium salt 102029-73-2 2.500
D-Glucose 50-99-7 1000.000
D-Glucuronic acid sodium salt 207569-96-4 3.880
FAD disodium salt 84366-81-4 0.106
Glutathione reduced 70-18-8 10.000
myo-Inositol 87-89-8 0.050
Phenol red sodium salt 34487-61-1 21.240
Thymidine 50-89-5 10.000
Tween 80 9005-65-6 5.000
Uridine-5-Triphosphate.Na 19817-92-6 1.000
β-NAD 53-84-9 7.000
β-NADP sodium salt 1184-16-3 1.000
Customization: pH, glucose concentration, salt balance, HEPES concentration, and full nutrient profile are available on request. Contact support@diagnocine.com with your specifications.
Quality Assurance

ISO 13485:2016 manufacturing & compliance

FluxMPS™ CMRL 1066 Medium w/o Sodium Bicarbonate: 1X Liquid is manufactured under a full ISO 13485:2016 quality management system, with final packaging, testing, and customization completed at Diagnocine Precision in Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering manufacturing, testing, and release for every production lot.

water_drop

Ultrapure Type 1 Water

All media prepared with 18.2 MΩ·cm resistivity water (ASTM D1193 / ISO 3696 Type I), with controlled trace-metal and TOC levels.

biotech

ISO Class 5 Fill & Finish

Aseptic filling performed in validated laminar-flow (ISO Class 5 / Class 100) workstations; 21 CFR Part 820 (QMSR) aligned.

assignment

Micro-Batch Precision

Small-batch manufacturing supports lot-to-lot nutrient consistency for reproducible perfusion studies and long-term OoC experiments.

Endotoxin — USP <85> BET

LAL assay performed per batch. Release specification: less than 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration particle count test confirms ≥10 µm and ≥25 µm particulate compliance on every lot.

Osmolality — USP <785>

Freezing-point osmometry performed per USP <785>. Result: Contact for specification.

Documentation — CoA & Full Lot Records

Certificate of Analysis available for every lot, including full QC panel, raw material traceability, and release signatures.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification less than 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.
Certificate of Analysis: Request your lot-specific CoA at support@diagnocine.com with your lot number and order reference.
Product Comparison

How DCP-CMRL-B1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered alternatives of the same base formulation.

Parameter DCP-CMRL-B1X (FluxMPS™) Conventional CMRL 1066 (0.22 µm) Standard CMRL 1066 alternative
Grade Microfluidics Suitable Standard grade Standard grade
Base Formulation CMRL 1066 w/o Sodium Bicarbonate: 1X Liquid CMRL 1066 Standard CMRL 1066 Equivalent
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 stages 1 stage 1–2 stages
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin (release specification) less than 0.05 EU/mL Corning classical liquid media — less than 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle USP <788> Method 1 cancel cancel
Water quality Ultrapure Type 1 (18.2 MΩ·cm) Purified water Purified water
Manufacturing QMS ISO 13485:2016 Variable Variable
Microfluidic channel compatibility check_circle Validated cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle On request cancel Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ CMRL 1066 Medium w/o Sodium Bicarbonate: 1X Liquid and Microfluidics Suitable cell culture media.

Yes. DCP-CMRL-B1X is Microfluidics Suitable, processed through our Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), yielding low particulate counts suited to OoC and MPS devices.
Standard 0.22 µm filtration does not retain mycoplasma (0.2–0.3 µm diameter) or subvisible particulates that can accumulate inside microchannels. FluxMPS™ uses four sequential stages reaching 0.04 µm, delivering approximately 5× lower particulate counts per USP <788> Method 1 (light obscuration).
This formulation is supplied without sodium bicarbonate and without HEPES so you can select your preferred buffering system. Add sodium bicarbonate for CO2-incubator culture, or HEPES for CO2-independent/open-system culture. Contact support@diagnocine.com for guidance or a custom pre-buffered formulation.
As supplied, this formulation contains no sodium bicarbonate and no HEPES. A buffering system must be added based on your intended CO2 environment: sodium bicarbonate for standard CO2 incubator use, or HEPES for CO2-independent culture. Contact support@diagnocine.com for supplementation guidance.
Yes. FluxMPS™ CMRL 1066 Medium w/o Sodium Bicarbonate: 1X Liquid can be supplemented with FBS, growth factors, antibiotics, or other additives per standard cell culture practice. When adding serum or protein-containing supplements, filter through a 0.2 µm low-protein-binding PES or PVDF membrane; do not use a 0.04 µm membrane for supplement filtration, as it will remove essential serum proteins. Add supplements immediately before use.
Endotoxin is controlled per manufacturing batch. Every batch is tested by LAL assay (USP <85> BET, assay sensitivity 0.005 EU/mL) before release and must meet the release specification of less than 0.05 EU/mL. Batch-specific results are documented in the Certificate of Analysis available on request from support@diagnocine.com.
Yes. A batch-specific CoA is available for every shipment and includes: appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility (USP <71>), particulate matter (USP <788> Method 1), raw material traceability, manufacturing date, and authorized release signatures. Request via support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed publications supporting the scientific rationale for ultra-filtered media and microfluidic cell culture applications.

  1. Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
  3. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
  4. Warburg O (1956). On the origin of cancer cells. Science, 123(3191), 309–314. doi:10.1126/science.123.3191.309
  5. Emmons EV (1965). Detection of mycoplasma in cell cultures using filtration. Proceedings of the Society for Experimental Biology, 118, 1010–1015. doi:10.3181/00379727-118-29988
  6. Kim S et al. (2012). Gut-on-a-chip microdevice replicates key functional features of the human intestine. Lab on a Chip, 12(12), 2165–2174. doi:10.1039/c2lc40074j
  7. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
  8. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
  9. Schuster B et al. (2020). Automated microfluidic platform for dynamic and combinatorial drug screening of tumor organoids. Nature Communications, 11, 5271. doi:10.1038/s41467-020-19058-4
  10. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175

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