FluxMPS™ CMRL 1066 Medium w/o Phenol Red: 1X Liquid
FluxMPS™ CMRL 1066 Medium w/o Phenol Red: 1X Liquid
FluxMPS™ CMRL 1066 Medium w/o Phenol Red: 1X Liquid is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. Processed through Diagnocine’s four-stage filtration train reaching a 0.04 µm final pore size, this phenol red-free CMRL 1066 formulation is built for microchannel-safe performance in imaging and biosensor-integrated culture platforms.
- Quadruple-stage nano-filtration: 0.1 µm (Prefiltration I & II) + 0.04 µm (Final filtration I & II), reaching a 0.04 µm terminal pore size
- Endotoxin release specification: < 0.05 EU/mL (USP <85> BET), controlled per manufacturing batch
- CMRL 1066 base formulation, phenol red-free; 1000 mg/L (1.0 g/L) glucose and 100 mg/L L-glutamine
- Sodium bicarbonate-buffered (2200 mg/L); requires approximately 5.8% CO₂ for physiological pH maintenance
- Prepared with Ultrapure Type 1 water (18.2 MΩ·cm); ISO Class 5 aseptic fill & finish
- Manufactured under an ISO 13485:2016 quality management system; final QC and customization completed at Diagnocine, Totowa, NJ
- Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request
- Glucose1000 mg/L (1.0 g/L)
- L-Glutamine100 mg/L
- Sodium PyruvateNone / Not added
- Phenol RedNone / Not added
- pH (USP <791>)7.4
- Osmolality (USP <785>)300 - 340 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2 (4 stages)
- Storage2–8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm-filtered media carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and batch-to-batch endotoxin variability that accumulate inside microchannels — corrupting biosensor readings, stressing primary cultures, and shortening device lifetimes. FluxMPS™ is built around a four-stage 0.04 µm filtration train to address these failure modes directly.
Microchannel-safe purity
0.04 µm final filter stage retains particles down to sub-mycoplasma size; USP <788> Method 1 particulate compliance verified per lot.
Total metabolic control
Fixed 1000 mg/L glucose and 100 mg/L L-glutamine support standard metabolic workflows, with custom carbon-source and nutrient concentrations available for flux-based studies.
Ultrapure-grade water
Prepared with Type 1 water (18.2 MΩ·cm), meeting ASTM D1193 Type I / ISO 3696 purity standards, with strict trace-metal and total organic carbon (TOC) control.
Low background for imaging
Phenol red-free, ultra-low particulate baseline supports confocal microscopy, live-cell biosensors, and TEER measurements without interference from filtration debris. Note: riboflavin (0.010 mg/L) contributes intrinsic fluorescence; account for it when configuring optical assays.
Rich, stable nutrient profile
Micro-batch precision manufacturing locks in amino acid and vitamin concentrations, ensuring lot-to-lot reproducibility critical for long-term perfusion studies.
Customization on demand
pH, glucose concentration, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
FluxMPS™ CMRL 1066 Medium w/o Phenol Red: 1X Liquid is processed through a four-stage serial filtration sequence that reaches a 0.04 µm final pore size — addressing mycoplasma-sized particulates and subvisible debris that 0.22 µm filtration alone cannot remove.
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1
0.1 µm Prefiltration I
Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm final-filter cartridge.
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2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.
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3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm final-filter cartridge from breakthrough.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill performed in a validated ISO Class 5 laminar-flow workstation.
Performance vs. conventional media
The train runs two dedicated 0.1 µm prefilter + 0.04 µm final-filter pairs in series, reaching a 0.04 µm terminal pore size with full USP <788> Method 1 (light obscuration) particulate compliance verified on every production lot.
© Diagnocine® — DCP-CMRL-R1X
Designed for next-generation cell culture platforms
FluxMPS™ CMRL 1066 Medium w/o Phenol Red: 1X Liquid is suited for organ-on-a-chip, imaging, and primary/stem cell models where particulate contamination, endotoxin variation, and phenol red interference are unacceptable.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant — a separate product line from this Microfluidics Suitable formulation — is available for automated bioreactor and robotic perfusion systems requiring the highest particulate exclusion.
- Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
- Valve & Sensor Protection: prevents particulate-induced blockage in precision fluidic systems
- Extended Perfusion Stability: maintains flow rate consistency across multi-week automated runs
Inquiry Required: The 0.01 µm MPS Grade variant is available by special order. Contact support@diagnocine.com to request this variant.
Micro Physiological System (MPS) & Chip
Ultra-filtered formulation reduces microchannel clogging risk and supports laminar flow integrity across complex chip geometries.
Cell Line Maintenance & Metabolic Studies
Defined 1000 mg/L glucose base and low-endotoxin background support reproducible cancer cell line culture and downstream metabolic assays.
iPSC-Derived Models
Ultrapure formulation supports sensitive iPSC differentiation protocols where endotoxin and particulates cause off-target effects.
Endothelial & Primary Cells
Microchannel-safe purity supports endothelial barrier integrity and TEER measurement stability in perfusion models.
Metabolic Flux Analysis
Defined, reproducible base composition supports isotope tracing and NMR-based metabolomics workflows. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium formulated to the XF specification.
Microscopy & Optical Sensing
Phenol red-free, ultra-low particulate load supports confocal imaging and optical biosensor integration; account for intrinsic riboflavin fluorescence when designing assays.
Lot-release quality parameters
Every production lot of FluxMPS™ CMRL 1066 Medium w/o Phenol Red: 1X Liquid undergoes the complete quality-release battery listed below before shipment.
| Parameter | Specification |
|---|---|
| Formulation | CMRL 1066 base + L-Glutamine + Sodium Bicarbonate + Calcium + Magnesium + Glucose; without Phenol Red, HEPES, Sodium Pyruvate |
| Appearance | Pale yellow, clear solution (phenol red-free) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 300 - 340 mOsm/kg H2O |
| Glucose | 1000 mg/L (1.0 g/L) |
| L-Glutamine | 100 mg/L |
| Sodium Pyruvate | None / Not added |
| Phenol Red | None / Not added |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch release specification) |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> | Compliant (Method 1, light obscuration) |
| Particulate ≥25 µm USP <788> | Compliant (Method 1, light obscuration) |
| Water Purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 ISO 13485 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, protected from light |
| Freeze-thaw | Not recommended |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | Approximately 5.8% CO₂ (derived from 2200 mg/L NaHCO₃ buffering at pH 7.4; verify per incubator) |
| Parameter | Specification |
|---|---|
| Raw material grade | Cell culture / reagent grade |
| Traceability | Full lot documentation, CoA available |
| Manufacturing QMS | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch precision manufacturing |
| Intended use | For Research Use Only (RUO) |
Full composition (mg/L)
Complete formulation with CAS numbers, reproduced from the manufacturer specification. 57 ingredient rows across 4 category groups. Custom compositions available on request.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.690 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium acetate anhydrous | 127-09-3 | 50.000 |
| Sodium bicarbonate | 144-55-8 | 2200.000 |
| Sodium chloride | 7647-14-5 | 6800.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 122.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 50.000 |
| L-Alanine | 56-41-7 | 25.000 |
| L-Arginine | 74-23-7 | 57.870 |
| L-Aspartic acid | 56-84-8 | 30.000 |
| L-Cysteine hydrochloride monohydrate | 7048-04-6 | 260.000 |
| L-Cystine dihydrochloride | 30189-89-0 | 20.000 |
| L-Glutamic acid | 56-86-0 | 75.000 |
| L-Glutamine | 56-85-9 | 100.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 20.000 |
| L-Isoleucine | 73-32-5 | 20.000 |
| L-Leucine | 61-90-5 | 60.000 |
| L-Lysine hydrochloride | 657-27-2 | 70.000 |
| L-Methionine | 63-68-3 | 15.000 |
| L-Phenylalanine | 63-91-2 | 25.000 |
| L-Proline | 147-85-3 | 40.000 |
| L-Serine | 56-45-1 | 25.000 |
| L-Threonine | 72-19-5 | 30.000 |
| L-Tryptophan | 73-22-3 | 10.000 |
| L-Tyrosine disodium salt dihydrate | 69847-45-6 | 40.000 |
| L-Valine | 72-18-4 | 25.000 |
| Trans-4-Hydroxy-L-Proline | 51-35-4 | 10.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 0.500 |
| D-Biotin | 58-85-5 | 0.010 |
| D-Pantothenate (Hemicalcium) | 137-08-6 | 0.010 |
| Folic acid | 59-30-3 | 0.010 |
| L-Ascorbic acid sodium salt | 134-03-2 | 50.000 |
| Nicotinamide | 98-92-0 | 0.025 |
| Nicotinic acid | 59-67-6 | 0.025 |
| Pyridoxal hydrochloride | 65-22-5 | 0.025 |
| Pyridoxine hydrochloride | 58-56-0 | 0.025 |
| Riboflavin | 83-88-5 | 0.010 |
| Thiamine hydrochloride | 67-03-8 | 0.010 |
| p-Amino benzoic acid (PABA) | 150-13-0 | 0.050 |
| OTHERS | ||
| myo-Inositol | 87-89-8 | 0.050 |
| 2' Deoxyadenosine | 958-09-8 | 10.000 |
| 2' Deoxycytidine hydrochloride 2' | 960-71-4 | 10.000 |
| Deoxyguanosine | 961-07-9 | 10.000 |
| 5-Methyldeoxycytidine | 838-07-3 | 0.100 |
| Cholesterol | 57-88-5 | 0.200 |
| Cocarboxylase | 154-87-0 | 1.000 |
| Coenzyme A sodium salt | 102029-73-2 | 2.500 |
| D-Glucose | 50-99-7 | 1000.000 |
| D-Glucuronic acid sodium salt | 207569-96-4 | 3.880 |
| FAD disodium salt | 84366-81-4 | 0.106 |
| Glutathione reduced | 70-18-8 | 10.000 |
| Thymidine | 50-89-5 | 10.000 |
| Tween 80 | 9005-65-6 | 5.000 |
| Uridine-5-Triphosphate.Na | 19817-92-6 | 1.000 |
| β-NAD | 53-84-9 | 7.000 |
| β-NADP sodium salt | 1184-16-3 | 1.000 |
ISO 13485:2016 manufacturing & compliance
FluxMPS™ CMRL 1066 Medium w/o Phenol Red: 1X Liquid is manufactured under a full ISO 13485:2016 quality management system, with final packaging, testing, and customization completed at Diagnocine in Totowa, New Jersey, USA.
ISO 13485:2016 QMS
Full quality management system certification covering all manufacturing, testing, and release processes for every production lot.
Ultrapure Type 1 Water
Media prepared with 18.2 MΩ·cm resistivity water meeting ASTM D1193 Type I / ISO 3696 purity standards, with strict trace-metal and total organic carbon (TOC) control.
ISO Class 5 Fill & Finish
Aseptic filling performed in validated laminar-flow (ISO Class 5 / Class 100) workstations; 21 CFR Part 820 (QMSR) aligned.
Micro-Batch Precision
Small-batch manufacturing locks in lot-to-lot nutrient consistency critical for reproducible perfusion studies and long-term OoC experiments.
Endotoxin — USP <85> BET
LAL assay performed per batch; assay sensitivity 0.005 EU/mL. Release specification: < 0.05 EU/mL.
Particulate — USP <788> Method 1
Light obscuration particle count test confirms ≥10 µm and ≥25 µm particulate compliance on every lot.
Osmolality — USP <785>
Freezing-point osmometry performed per USP <785>. Result: 300 - 340 mOsm/kg H2O.
Documentation — CoA & Full Lot Records
Certificate of Analysis available for every lot, including full QC panel, raw material traceability, and release signatures.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-CMRL-R1X (FluxMPS™) compares
Side-by-side comparison against conventional 0.22 µm-filtered alternatives of the same base formulation.
| Parameter | DCP-CMRL-R1X (FluxMPS™) | Conventional CMRL (0.22 µm) | Standard CMRL alternative |
|---|---|---|---|
| Grade | Microfluidics Suitable | Standard grade (0.22 µm) | Standard grade (0.22 µm) |
| Base Formulation | CMRL 1066 w/o Phenol Red | CMRL Standard | CMRL Equivalent |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 stages | 1 stage | 1–2 stages |
| Mycoplasma barrier filtration | check_circle | cancel | cancel |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP particulate compliance | check_circle USP <788> Method 1 | cancel | cancel |
| Water quality | Ultrapure Type 1 (18.2 MΩ) | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | Variable | Variable |
| Microfluidic channel compatibility | check_circle Validated | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle On request | cancel | Limited |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ CMRL 1066 Medium w/o Phenol Red: 1X Liquid.
Supporting literature
Peer-reviewed publications supporting the scientific rationale for ultra-filtered media and microfluidic cell culture applications.
- Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
- Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
- Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
- Emmons EV (1965). Detection of mycoplasma in cell cultures using filtration. Proceedings of the Society for Experimental Biology, 118, 1010–1015. doi:10.3181/00379727-118-29988
- Kim S et al. (2012). Gut-on-a-chip microdevice replicates key functional features of the human intestine. Lab on a Chip, 12(12), 2165–2174. doi:10.1039/c2lc40074j
- Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated and continual in situ monitoring of organoid behaviors. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
- Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
- Schuster B et al. (2020). Automated microfluidic platform for dynamic and combinatorial drug screening of tumor organoids. Nature Communications, 11, 5271. doi:10.1038/s41467-020-19058-4
- Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175
- Parham MB (1963). Studies on the composition of CMRL 1066 medium. Annals of the New York Academy of Sciences, 139, 132–144. doi:10.1111/j.1749-6632.1961.tb18994.x
