Flamma® 749 PEG4-Alkyne
Size of product (mg)
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1 mg
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5 mg
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25 mg
Flamma® 749 PEG4-Alkyne
Cat. No. List below
Description
The dye exhibits excitation and emission maxima at 749 nm and 774 nm, respectively, positioning it firmly in the NIR region of the spectrum. These spectral characteristics are comparable to other popular NIR dyes such as Alexa 750, Cy7, IRDye 750, and DyLight 755. Flamma 749 can be efficiently excited using a 750 nm laser line or dye-pumped laser, with emission occurring in the NIR region. This spectral range is particularly advantageous for biological tissue imaging due to improved penetration and reduced autofluorescence.
Flamma® Fluors 749 PEG4-alkyne participates in CuAAC reactions, coupling with azide-functionalized biomolecules to form 1,4-disubstituted 1,2,3-triazoles. This reaction occurs efficiently in living systems without interfering with native biochemical processes. To utilize this dye, researchers must first introduce azide functionality onto the target biomolecule through chemical or genetic modification.
This reagent is particularly useful for cellular imaging (provides stable fluorescence signals in various biological imaging applications), nucleotide functionalization (allows for specific labeling of nucleic acids, bioconjugation (enables the attachment of the dye to various biomolecules through click chemistry), and in vivo imaging (the NIR properties make it suitable for deep tissue imaging).
The PEG4 spacer in this dye offers several advantages:
1. Improved water solubility
2. Reduced aggregation
3. Enhanced biocompatibility
4. Increased flexibility for bioconjugation
These properties make Flamma® Fluors 749 PEG4-alkyne a valuable tool for researchers in fields such as molecular biology, biochemistry, and biomedical imaging. Its unique reactivity, combined with its NIR spectral properties, expands the toolkit available for bioconjugation and probe development, particularly for studying complex biological systems in living organisms.
- Fluorophore: Flamma® Fluors 749
- Reactive group: PEG4-alkyne
- Excitation/Emission Max.(nm): 749/774
- Spectrally similar dyes: Alexa750, DyLight755, Cy7, IRDye750
- Extinction coefficient: ≥ 220,000 cm-1M-1
- Appearance: Green Solid
- Molecular Weight: 924.17 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWG1001 | Flamma® 496 PEG4-Alkyne | 496 | 520 | Alexa488, FITC, Cy2 |
| PWG1122 | Flamma® 552 PEG4-Alkyne | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWG1415 | Flamma® 581 PEG4-Alkyne | 581 | 596 | Alexa594, DyLight594 |
| PWG1215 | Flamma® 648 PEG4-Alkyne | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWG1515 | Flamma® 675 PEG4-Alkyne | 674 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| PWG1301 | Flamma® 749 PEG4-Alkyne | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWG1603 | Flamma® 774 PEG4-Alkyne | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors for Click Chemistry
The most widely utilized click chemistry is 1,3-dipolar cycloaddition between an azide and an alkyne to produce 1,4-disubstituted 1,2,3-triazole. There are two types of 1,3-dipolar cycloaddition methods: copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC). BioActs offers Flamma® Fluors Alkyne dyes for CuAAC, Flamma® Fluors ADIBO products for SPAAC and Flamma® Fluors Azide dyes for both CuAAC and SPAAC.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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