Flamma® 648 PEG4-Alkyne
Size of product (mg)
-
1 mg
-
5 mg
-
25 mg
Flamma® 648 PEG4-Alkyne
Cat. No. List below
Description
Flamma® Fluors 648 PEG4-Alkyne is a cutting-edge far-red fluorescent dye specifically engineered for superior performance in copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reactions. This innovative reagent combines the power of click chemistry with exceptional fluorescence properties and improved biocompatibility, offering researchers a versatile and efficient tool for advanced cellular imaging and biomolecule labeling.
Key Features and Advantages:
- Optimal Spectral Properties: Excitation/emission maxima at 648/663 nm, comparable to popular dyes like Alexa 647, Cy5, and DyLight 650.
- Versatile Excitation: Compatible with 593 or 633 nm laser lines, providing flexibility in imaging setups.
- Superior Brightness: High extinction coefficient of ≥ 227,000 cm-1M-1 ensures vivid fluorescence signals.
- Minimal Protein Interference: Low CF280 value of 0.023 reduces interference with protein absorbance measurements.
- Enhanced Biocompatibility: Tetraethylene glycol (PEG4) spacer improves solubility and reduces non-specific binding.
- Bioorthogonal Labeling: Forms 1,4-disubstituted 1,2,3-triazoles without disrupting native biochemical processes.
Flamma® Fluors 648 PEG4-Alkyne excels in various applications, including: advanced cellular imaging (ideal for high-resolution live-cell and fixed-cell microscopy), nucleotide functionalization (enables specific labeling of modified nucleic acids with improved accessibility), protein labeling (excellent for tracking proteins in complex biological systems with reduced background), biomolecule tracking (suitable for studying dynamics of various biomolecules in living systems with enhanced specificity).
The PEG4 Spacer Advantage incorporates a tetraethylene glycol (PEG4) spacer between the dye and the alkyne group offers several benefits. The first one is improved solubility, which enhances the dye's solubility in aqueous environments. The scond advantage is it reduces steric hindrance, which provides better access to the alkyne group for more efficient click reactions. The third one is minimized non-specific binding , which decreases unwanted interactions with cellular components.
Finally, the enhanced conjugate stability improves the overall stability of the labeled biomolecules.
For optimal results, introduce azide functionality to target biomolecules via chemical or genetic modification. Perform CuAAC reaction under optimized conditions (e.g., copper catalyst, reducing agent, ligand), purify labeled biomolecules using standard chromatographic techniques, and verify labeling efficiency through spectrophotometric analysis.
- Fluorophore: Flamma® Fluors 648
- Reactive group: PEG4-alkyne
- Excitation/Emission Max.(nm): 648/663
- Spectrally similar dyes: Alexa647, DyLight650, Cy5
- Extinction coefficient: ≥ 227,000 cm-1M-1
- CF280: 0.023
- Appearance: Blue Solid
- Molecular Weight: 898.14 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWG1001 | Flamma® 496 PEG4-Alkyne | 496 | 520 | Alexa488, FITC, Cy2 |
| PWG1122 | Flamma® 552 PEG4-Alkyne | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWG1415 | Flamma® 581 PEG4-Alkyne | 581 | 596 | Alexa594, DyLight594 |
| PWG1215 | Flamma® 648 PEG4-Alkyne | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWG1515 | Flamma® 675 PEG4-Alkyne | 674 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| PWG1301 | Flamma® 749 PEG4-Alkyne | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWG1603 | Flamma® 774 PEG4-Alkyne | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
- Advanced Technology: Combines click chemistry with far-red fluorescence and PEG4 spacer for superior bioimaging.
- Exceptional Brightness: High extinction coefficient for clear, vivid signals even at low concentrations.
- Improved Biocompatibility: PEG4 spacer enhances solubility and reduces non-specific interactions.
- Versatility: Suitable for various biomolecules and advanced imaging techniques.
- Minimal Background: Low CF280 value ensures high signal-to-noise ratio in protein studies.
Flamma® Fluors for Click Chemistry
The most widely utilized click chemistry is 1,3-dipolar cycloaddition between an azide and an alkyne to produce 1,4-disubstituted 1,2,3-triazole. There are two types of 1,3-dipolar cycloaddition methods: copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC). BioActs offers Flamma® Fluors Alkyne dyes for CuAAC, Flamma® Fluors ADIBO products for SPAAC and Flamma® Fluors Azide dyes for both CuAAC and SPAAC.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
1. Xu, Peisheng. Zwitterionic chitosan derivatives for pH-sensitive stealth coating. Biomacromolecules 11.9 (2010): 2352-2358.
2. Ibrahim, Basma M. A strategy to deliver genes to cystic fibrosis lungs: a battle with environment. Journal of controlled release 155.2 (2011): 289-295.
3. Oh, Keun Sang. Accurate sequential detection of primary tumor and metastatic lymphatics using a temperature-induced phase transition nanoparticulate system. International journal of nanomedicine 9 (2014): 2955.
4. Yhee, Ji Young. Tumor-targeting transferrin nanoparticles for systemic polymerized siRNA delivery in tumor-bearing mice. Bioconjugate chemistry 24.11 (2013): 1850-1860.
5. Yoon, Hong Yeol. Glycol chitosan nanoparticles as specialized cancer therapeutic vehicles: Sequential delivery of doxorubicin and Bcl-2 siRNA. Scientific reports 4 (2014).
6. Ryu, Ju Hee. Early diagnosis of arthritis in mice with collagen?induced arthritis, using a fluorogenic matrix metalloproteinase 3–specific polymeric probe. Arthritis & Rheumatism 63.12 (2011): 3824-3832.
7. Hollis, Christin P. In vivo investigation of hybrid paclitaxel nanocrystals with dual fluorescent probes for cancer theranostics. Pharmaceutical research 31.6 (2014): 1450-1459.
8. Koo, Heebeom. The movement of self-assembled amphiphilic polymeric nanoparticles in the vitreous and retina after intravitreal injection. Biomaterials 33.12 (2012): 3485-3493.
9. Zhu, Lei. Real-time monitoring of caspase cascade activation in living cells. Journal of controlled release 163.1 (2012): 55-62.
10. Yoon, Hong Yeol. Bioreducible hyaluronic acid conjugates as siRNA carrier for tumor targeting. Journal of Controlled Release 172.3 (2013): 653-661.
11. Yhee, Ji Young. Cancer-targeted MDR-1 siRNA delivery using self-cross-linked glycol chitosan nanoparticles to overcome drug resistance. Journal of Controlled Release 198 (2015): 1-9.
12. Park, Jin Woo. Wide-Ranged Fluorescent Molecular Weight Size Markers for Electrophoresis. Bulletin of the Korean Chemical Society 34.1 (2013): 29-30.
13. Huang, Xinglu. Multiplex Imaging of an Intracellular Proteolytic Cascade by using a Broad?Spectrum Nanoquencher. Angewandte Chemie International Edition 51.7 (2012): 1625-1630.
