Flamma® 675 Alkyne
Size of product (mg)
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1 mg
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5 mg
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25 mg
Flamma® 675 Alkyne
Cat. No. List below
Description
The dye exhibits maximal excitation at 675 nm and emission at 691 nm, aligning closely with popular NIR dyes such as Alexa 680, Cy5.5, IRDye 680LT, and DyLight 680. This spectral similarity facilitates seamless integration into existing imaging protocols. Flamma 675 can be efficiently excited using a 633 nm laser line, with emission occurring in the NIR region, which is advantageous for deep tissue penetration in biological samples.
Flamma 675 Alkyne is specifically designed for copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) reactions, a key component of click chemistry. The alkyne group reacts with azides to form 1,4-disubstituted 1,2,3-triazoles, allowing for bioorthogonal labeling inside living systems without interfering with native biochemical processes.
Flamma 675 Alkyne excels in various biomedical research application such as cellular imaging, nucleotide functionalization, bioorthogonal labeling in living systems, and In vitro and in vivo imaging studies.
Flamma 675 Alkyne offers comparable spectral properties to other popular NIR dyes, making it a suitable alternative or complement in multi-color imaging experiments. Its click chemistry compatibility sets it apart for specific applications requiring bioorthogonal labeling.
The click chemistry approach using Flamma 675 Alkyne offers several advantages in biological research like:
1. Specificity: The CuAAC reaction is highly specific, reducing off-target labeling.
2. Mild Conditions: The reaction can occur under physiological conditions, preserving biological function.
3. Versatility: A wide range of azide-modified biomolecules can be labeled, including proteins, nucleic acids, and glycans.
NOTE: The CuAAC reaction is highly efficient, the use of copper catalysts may require optimization to minimize potential toxicity in live-cell applications. Alternative copper-free click chemistry methods might be considered for particularly sensitive systems.The high extinction coefficient (≥ 200,000 cm^-1^M^-1^) indicates strong light absorption, contributing to the dye's sensitivity in detection applications.
Flamma® Fluors 675 Alkyne combines the power of click chemistry with the advantages of NIR fluorescence, making it a valuable tool for researchers in chemical biology, cell biology, and biomedical imaging. Its ability to perform bioorthogonal labeling opens up new possibilities for studying complex biological systems and processes with minimal interference.
- Fluorophore: Flamma® Fluors 675
Reactive group: Alkyne
Excitation/Emission Max.(nm): 675/691
Spectrally similar dyes: Alexa680, DyLight680, Cy5.5, IRDye680LT
Extinction coefficient: ≥ 200,000 cm-1M-1
CF280: 0.1
Appearance: Blue Solid
Molecular Weight: 982.17 g/mol
Solubility: DMF, DMSO
Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | FSD series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| CWK1001 | Flamma® 496 Alkyne | 496 | 520 | Alexa488, FITC, Cy2 |
| PWK1122 | Flamma® 552 Alkyne | 550 | 564 | Alexa555, DyLight549, Cy3, ATTO550 |
| KWK1415 | Flamma® 581 Alkyne | 581 | 596 | Alexa594, DyLight594 |
| PWK1215 | Flamma® 648 Alkyne | 648 | 663 | Alexa647, DyLight650, Cy5 |
| PWK1515 | Flamma® 675 Alkyne | 674 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT |
| PWK1301 | Flamma® 749 Alkyne | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWK1603 | Flamma® 774 Alkyne | 774 | 800 | Cy7.5, DyLight800, IRDye800 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
What are the advantages?
- Bioorthogonal Reactivity: Enables specific labeling in complex biological environments.
- NIR Emission: Allows for deep tissue penetration and reduced autofluorescence in biological samples.
- Stability: Provides consistent and reliable fluorescence signals.
- Versatility: Compatible with various azide-modified biomolecules and imaging techniques.
Flamma® Fluors for Click Chemistry
The most widely utilized click chemistry is 1,3-dipolar cycloaddition between an azide and an alkyne to produce 1,4-disubstituted 1,2,3-triazole. There are two types of 1,3-dipolar cycloaddition methods: copper(I)-catalyzed azide-alkyne cycloaddition (CuAAC) and strain-promoted azide-alkyne cycloaddition (SPAAC). BioActs offers Flamma® Fluors Alkyne dyes for CuAAC, Flamma® Fluors ADIBO products for SPAAC and Flamma® Fluors Azide dyes for both CuAAC and SPAAC.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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