The endo free EZgeneTM 96-well plasmid kit provides an easy and fast method for isolating endofree high quality plasmid DNA in a high through put format. The key to this system is Biomiga’s proprietary endo removal buffer system that avidly, but reversibly, binds DNA to ezbind matrix under optimized condition while proteins and other unwanted contaminants are removed by wash buffer. High quality plasmid DNAs are eluted with endofree water. By using the 96-well kit, 96 samples can be simultaneously processed in less than 90 minutes. The purified DNA is ready for transfection of endotoxin sensitive cell lines in high-through-put format.
Note: The yield of plasmid DNA depends on the origin of the replication and the size of the plasmid. The protocols are optimized for high copy number plasmid purification. For low copy number plasmids, both the culture volume and the buffer volume need to be scaled up 3 to 5 times. Please contact our customer service for further information and reference the table below for the commonly used plasmids.
Storage and Stability
All components are guaranteed for 24 months from the date of purchase. The Buffer A1/RNase A should be stored at 4°C. Buffer B1 needs to be tightly capped to avoid oxidation.
