E-cad Fc Plate(96well)
Cat. No.:
FNK-hECP-96w
Size: 1piece
Storage: Avoid direct sunlight and store at room temperature in a dry place.
Specifications:
- This product is for research use only and cannot be used for clinical or diagnostic purposes.
- This product is produced aseptically.
- After opening, use promptly.
- Prepare DPBS containing calcium and magnesium.
Protein Description:
This is a recombinant protein in which the Fc region of an antibody is fused to the C-terminus of the human E-cadherin extracellular domain (Asp155 to Lys697), produced in 293 cells. This E-cadherin Fc protein has been purified and orientationally coated on the surface of polystyrene culture plates with the N-terminus of cadherin facing upward. It has been confirmed that multiple types of human iPS cells can proliferate while maintaining their undifferentiated and pluripotent states on the E-cadherin Fc plate.
DPBS (+) Composition
|
Component
|
Molecular Weight
|
Final Concentration (mM)
|
|
Calcium Chloride (CaCl₂) (anhydrous)
|
111.0
|
0.901
|
|
Magnesium Chloride (MgCl₂·6H₂O)
|
203.0
|
0.493
|
|
Potassium Chloride (KCl)
|
75.0
|
2.667
|
|
Potassium Phosphate monobasic (KH₂PO₄)
|
136.0
|
1.471
|
|
Sodium Chloride (NaCl)
|
58.0
|
137.931
|
|
Sodium Phosphate dibasic (Na₂HPO₄·7H₂O)
|
268.0
|
8.060
|
Reference:
1) Dulbecco, R. and Vogt, M., (1954) Plaque formation and isolation of pure lines with Poliomyelitis viruses. J. Exp. Med., 98:167.
How To Use This Product:
-
Remove the E-cadherin Fc plate from the bag and add sterilized DPBS (+) to each well. Refer to the below table for the addition volumes.
| Cell Culture Area (cm²/well) |
DPBS (Mg,Ca) Volume (mL/well) |
| 0.35 |
0.1 |
-
Prepare the cell suspension for seeding.
-
Remove the DPBS (+) from the E-cadherin Fc plate and immediately seed the cells without letting the plate dry. If the culture surface of the plate dries, the activity of E-cadherin will decrease.
Detachment Method for Human iPS Cells Cultured on This Product
-
Remove the medium from the plate and rinse with sterilized DPBS without calcium and magnesium.
-
Add an appropriate amount of 0.5 mM EDTA in PBS and incubate in a 37°C incubator for about 2-3 minutes.
*Adjust the incubation time depending on the cells used.
-
Add medium containing 10 mM Y-27632 to the cells, collect by pipetting, centrifuge, and remove the supernatant.
*Use the dedicated medium suitable for your cells.
