Description:
Fast Growth: JM109, a K strain bacterium, carries mutations in recA and endA, leading to minimal recombination and improved quality of isolated plasmid DNA. In addition, the cells carry the F’ episome which allows blue/white screening for recombinant DNA. Thus, it has been a popular strain for routine subcloning. The cells can also be utilized for single-stranded DNA rescue when M13 or phagemid systems are used simultaneously. The JM109 strain is sensitive to all common antibiotics.
Theses products can become a powerful cloning tool when it is used in combination with Yeastern’s rapid ligation kit (#FYC003-100R). The ligation can be finished within 5 minutes and followed by 1-minute transformation. The fast growth rate of JM109 allows colonies to show up within 8 hours. Try this strain when you are in urgent.
Features
- Rapid growing strain
- Carries F’ episome
Applications
- Preparation of ssDNA
- Construction of gene library
- Subcloning
Genotype
e14
–(
McrA
–)
recA1
endA1
gyrA96
thi-1
hsdR17(rK
– mK
+)
supE44
relA1 D(
lac–
proAB) [F’
traD36
proAB
lacIqZDM15]
| Image |
Cat.No: |
Control Plasmid |
Control Efficiency |
Storage |
Quantity |
Link |
 |
YBB-JM109-FYE707-10VL |
5 μl (10-4 μg/μl) |
>5 x 107 cfu/μg |
-70°C ~ -80°C |
10 Vials |
 |
| YBB-JM109-FYE707-80VL |
80 Vials |
 |
| YBB-JM109-FYE709-10VL |
>5 x 108 cfu/μg |
10 Vials |
 |
| YBB-JM109-FYE709-80VL |
80 Vials |
 |