Endo H
Cat. No.: FNK-RP801-250
Size: 250000units
Storage: -20℃
Description:
EndoH(Endoglycosidase H) is a recombinant glycosidase from Streptomyces plicatus which cleaves within the chitobiose core of high mannose and some hybrid oligosaccharides from C-linked glycoproteins.
Specifications:
Supplied Reagents
EndoH
10 X Endo H Reaction Buffer
10 X Denaturing Solution
Concentration: 500,000units/mL
Storage Buffer:
20 mM Tris-HCl (pH7.5 at 25℃)
50 mM NaCl
5 mM EDTA
10 X Endo H Reaction Buffer: 0.5 M Sodium Acetate(pH6.0 at 25℃)
10 X Denaturing Buffer: 5% SDS, 0.4M DTT
Source: Recombinant protein with a N-terminal DYKDDDDK tag and 6xHis tag, expressed in E.coli.
Molecular Weight: 31kDa.
Purity: > 95%, as determined by SDS-PAGE visualized by CBB stain.
Unit Definition: One unit is defined as the amount of enzyme required to remove >95% of the carbohydrate from 10µg of denatured RNase B in 1hour at 37℃ in a total reaction volume of 10µl.
Standard Procedure:
1. Combine 1-20 μg of glycoprotein, 1 μl of 10X Denaturing Buffer and H20 (if necessary) to make a 10 μl total reaction volume.
2. Denature glycoprotein by heating reaction at 100℃ for 10 minutes.
3. Make a total reaction volume of 20 μl by adding 2 μl of 10X Endo H Reaction Buffer, H20 and 1-5 μl Endo H.
4. Incubate at 37℃ for 1 hour
References:
1) Maley, F. et al. (1989). Anal. Biochem. 180, 195-204.
2) Robbins, P. et al. (1984). J. Biol. Chem. 259, 7577- 7583.
| For Research Use Only. Not for use in diagnostic procedures. |
