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FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with 15mM HEPES w/o Sodium Bicarbonate: 1X Liquid

Product#: DCP-DM12H-BT1X
$49.50
DCP-DM12H-BT1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with 15mM HEPES w/o Sodium Bicarbonate: 1X Liquid

Contains L-Glutamine Contains Phenol Red Contains HEPES Contains Calcium Contains Magnesium Contains Glucose Contains Sodium Pyruvate Without Sodium Bicarbonate

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with 15mM HEPES w/o Sodium Bicarbonate: 1X Liquid is a Microfluidics Suitable, ultra-filtered cell culture medium engineered for organ-on-a-chip (OoC), tissue-on-a-chip (ToC), and microphysiological system (MPS) applications. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • Quadruple-stage filtration: 0.1 µm (Prefiltration I & II) + 0.04 µm (Final filtration I & II), reaching a 0.04 µm final cut-off
  • Endotoxin release specification: < 0.05 EU/mL (LAL assay, USP <85> BET)
  • DMEM/F-12 (1:1) base with 15 mM HEPES (3574.5 mg/L); bicarbonate-free, HEPES-buffered formulation with reduced CO₂ dependence
  • Phenol Red present (8.630 mg/L) for visual pH monitoring — consider background fluorescence for sensitive imaging assays
  • Glucose 3151 mg/L, L-Glutamine 365 mg/L, and Sodium Pyruvate 110 mg/L supplied ready to use
  • Manufactured under an ISO 13485:2016 quality management system; final packaging and QC at Diagnocine, Totowa, NJ
  • Custom formulations available — pH, glucose, salts, HEPES, and nutrient composition on request
DCP-DM12H-BT1X |Sizes: 500 mL, 1000 mL |Cell Culture Media
UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with 15mM HEPES w/o Sodium Bicarbonate: 1X Liquid
  • Glucose3151 mg/L (3.151 g/L)
  • L-Glutamine365 mg/L
  • Sodium Pyruvate110 mg/L
  • HEPES3574.5 mg/L (15 mM)
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)See CoA
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm-filtered media can carry mycoplasma-sized particulates (0.2–0.3 µm), subvisible debris, and endotoxin variability that accumulate inside microchannels — potentially corrupting biosensor readings, triggering inflammation in primary cultures, and shortening device lifetimes. FluxMPS™ is engineered to address these failure modes through validated quadruple-stage filtration to a 0.04 µm final cut-off.

filter_alt

Microchannel-safe purity

0.04 µm final filter retains particles to sub-mycoplasma size; USP <788> Method 1 particulate compliance verified per batch.

target

Total metabolic control

Defined carbon source (glucose, sodium pyruvate) and precise nutrient concentrations support metabolic flux experiments and Warburg-related applications.

water_drop

Ultrapure-grade water

Prepared with Type 1 ultrapure water (18.2 MΩ·cm) meeting ASTM D1193 specifications, supporting low trace-metal and organic carbon content.

visibility

Low background for imaging

Ultra-low particulate load supports confocal microscopy, live-cell biosensors, and TEER measurements; note this formulation contains phenol red (8.630 mg/L), which may contribute background fluorescence in sensitive imaging assays.

science

Rich, stable nutrient profile

Micro-batch precision locks in amino acid and vitamin concentrations for lot-to-lot reproducibility in long-term perfusion studies.

tune

Customization on demand

pH, glucose, salts, HEPES, and full nutrient composition available on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with 15mM HEPES w/o Sodium Bicarbonate: 1X Liquid is processed through a validated four-stage filtration sequence reaching a 0.04 µm final cut-off, addressing mycoplasma-sized particulates and subvisible particles that 0.22 µm filtration does not retain.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates and aggregates; protects the first 0.04 µm cartridge downstream.

  2. 2

    0.04 µm Final filtration I

    Retains sub-micron particulates and microaggregates, including mycoplasma-sized particles (0.2–0.3 µm), that pass a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge in the train.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish under ISO Class 5 conditions.

Performance vs. conventional media

Four sequential stages reaching a 0.04 µm final cut-off deliver approximately 5× cleaner media by particulate count compared to single-pass 0.22 µm filtration, with USP <788> Method 1 compliance verified on every batch.

5×
 
0.04
µm final filter pore size — sub-mycoplasma polishing
Sterility assurance: Every batch undergoes 14-day USP <71> sterility testing. Mycoplasma risk is controlled by 0.1 µm mycoplasma-retentive filtration (not tested per lot via a mycoplasma-specific assay unless otherwise stated on the Certificate of Analysis).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ Dulbecco's Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with 15mM HEPES w/o Sodium Bicarbonate 1X Liquid (DCP-DM12H-BT1X) Quadruple-stage filtration system: 0.1 μm Prefiltration I, 0.04 μm Final filtration I, 0.1 μm Prefiltration II, 0.04 μm Final filtration II for organ-on-a-chip and microfluidic applications by Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration architecture (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-DM12H-BT1X
Applications

Designed for next-generation cell culture platforms

FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with 15mM HEPES w/o Sodium Bicarbonate: 1X Liquid is validated for organ-on-a-chip, metabolic research, live-cell imaging, and primary cell models where particulate contamination and endotoxin variation are unacceptable.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra nano-filtered variant — Diagnocine's MPS Grade line — is available for automated bioreactor and robotic perfusion systems requiring the ultimate particulate exclusion.

  • Total Particulate Exclusion: 10 nm filtration for nanoscale valve and sensor protection
  • Valve & Sensor Protection: reduces risk of particulate-induced blockage in precision fluidic systems
  • Extended Perfusion Stability: supports flow rate consistency across multi-week automated runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm MPS Grade variant.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-filtered formulation is designed to reduce the risk of microchannel clogging and support laminar flow integrity.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Defined carbon source and a low-endotoxin release specification (< 0.05 EU/mL) support precise metabolic flux analysis.

MCF-7MDA-MB-231HeLaHT-1080
Stem Cell Biology

iPSC-Derived Models

Ultrapure formulation supports sensitive iPSC differentiation protocols where endotoxin variability can cause off-target effects.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Microchannel-safe purity supports endothelial barrier integrity and TEER measurement stability.

HUVECsBHK-21Primary fibroblasts
Metabolomics

Metabolic Flux Analysis

Defined nutrient profile supports isotope tracing and metabolic flux experiments. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

13C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

Low particulate background suited to confocal imaging and biosensor integration; phenol red content should be considered for fluorescence-sensitive assays.

ConfocalBiosensorsTEER
Technical Specifications

Lot-release quality parameters

Every production batch of FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with 15mM HEPES w/o Sodium Bicarbonate: 1X Liquid undergoes the complete quality-release battery below before shipment.

Physical & Chemical Parameters
Parameter Specification
Formulation Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with 15mM HEPES w/o Sodium Bicarbonate: 1X Liquid
Appearance Red-colored, clear solution
pH USP <791> 7.4
Osmolality USP <785> See CoA
Glucose 3151 mg/L (3.151 g/L)
L-Glutamine 365 mg/L
Sodium Pyruvate 110 mg/L
Phenol Red 8.630 mg/L (present)
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 Compliant
Particulate ≥25 µm USP <788> Method 1 Compliant
Water Purity Ultrapure Type 1, 18.2 MΩ·cm (ASTM D1193 / ISO 3696)
Manufacturing std. ISO 13485 ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protected from light
Freeze-thaw Not recommended
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement HEPES-buffered; reduced CO₂ dependence (validate per cell line)
Pack sizes available 500 mL, 1000 mL
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Cell culture / reagent grade
Traceability Full lot documentation, CoA available
Manufacturing QMS ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch precision manufacturing
Intended use For Research Use Only (RUO)
Formulation

Full composition (mg/L)

Complete formulation with CAS numbers, reproduced from manufacturer specification. Total: 52 components across 4 categories (Inorganic Salts, Amino Acids, Vitamins, Others). Custom compositions available on request.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 154.500
Copper sulfate pentahydrate 7758-99-8 0.0013
Disodium hydrogen phosphate 7558-79-4 71.020
Ferric nitrate nonahydrate 7782-61-8 0.050
Ferrous sulfate heptahydrate 7782-63-0 0.417
Magnesium chloride hexahydrate 7791-18-6 61.200
Magnesium sulfate anhydrous 7487-88-9 48.840
Potassium chloride 7447-40-7 311.800
Sodium chloride 7647-14-5 6996.000
Sodium dihydrogen phosphate monohydrate 10049-21-5 54.300
Zinc sulfate heptahydrate 7446-20-0 0.432
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 18.750
L-Alanine 56-41-7 4.450
L-Arginine hydrochloride 1119-34-2 147.500
L-Asparagine monohydrate 5794-13-8 7.500
L-Aspartic acid 56-84-8 6.650
L-Cysteine dihydrochloride 7048-04-6 31.290
L-Cystine hydrochloride monohydrate   17.560
L-Glutamic acid 56-86-0 7.350
L-Glutamine 56-85-9 365.000
L-Histidine hydrochloride monohydrate 5934-29-2 31.480
L-Isoleucine 73-32-5 54.470
L-Leucine 61-90-5 59.050
L-Lysine hydrochloride 657-27-2 91.250
L-Methionine 63-68-3 17.240
L-Phenylalanine 63-91-2 35.480
L-Proline 147-85-3 17.250
L-Serine 56-45-1 26.250
L-Threonine 72-19-5 53.450
L-Tryptophan 73-22-3 9.020
L-Tyrosine disodium salt 69847-45-6 48.100
L-Valine 72-18-4 52.850
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 8.980
D-Biotin 58-85-5 0.0035
D-Ca-Pantothenate 137-08-6 2.240
Folic acid 59-30-3 2.660
Nicotinamide 98-92-0 2.020
Pyridoxal hydrochloride 65-22-5 2.000
Pyridoxine hydrochloride 58-56-0 0.031
Riboflavin 83-88-5 0.219
Thiamine hydrochloride 67-03-8 2.170
Vitamin B12 68-19-9 0.680
OTHERS
D-Glucose 50-99-7 3151.000
DL-Thioctic acid 1200-22-2 0.105
HEPES buffer 7365-45-9 3574.500
Hypoxanthine sodium salt   2.400
Linoleic acid 60-33-3 0.042
myo-Inositol 87-89-8 12.600
Phenol red sodium salt 34487-61-1 8.630
Putrescine hydrochloride 333-93-7 0.081
Sodium pyruvate 113-24-6 110.000
Thymidine 50-89-5 0.365
Customization: pH, glucose, salt balance, HEPES, and full nutrient profile available on request. Contact support@diagnocine.com.
Quality Assurance

ISO 13485:2016 manufacturing & compliance

Manufactured under ISO 13485:2016 QMS with final packaging, testing, and customization at Diagnocine Precision in Totowa, New Jersey, USA.

verified

ISO 13485:2016 QMS

Full quality management system certification covering manufacturing, testing, and release for every production batch.

water_drop

Ultrapure Type 1 Water

All media prepared with 18.2 MΩ·cm resistivity Type 1 water meeting ASTM D1193 specifications.

biotech

ISO Class 5 Fill & Finish

Aseptic filling in validated ISO Class 5 laminar-flow workstations; 21 CFR Part 820 (QMSR) aligned.

assignment

Micro-Batch Precision

Small-batch manufacturing supports lot-to-lot nutrient consistency for reproducible perfusion studies.

Endotoxin — USP <85> BET

LAL assay per batch. Release specification: < 0.05 EU/mL; assay sensitivity 0.005 EU/mL.

Particulate — USP <788> Method 1

Light obscuration particle count confirms ≥10 µm and ≥25 µm compliance per batch.

Osmolality — USP <785>

Freezing-point osmometry per USP <785>. Result: See CoA.

Documentation — CoA & Full Lot Records

Certificate of Analysis with full QC panel, traceability, and release signatures for every batch.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request via support@diagnocine.com with your lot number.
Product Comparison

How DCP-DM12H-BT1X (FluxMPS™) compares

Side-by-side comparison against conventional 0.22 µm-filtered alternatives of the same base formulation.

Parameter DCP-DM12H-BT1X (FluxMPS™) Conventional DMEM/F-12 (0.22 µm) Standard DMEM/F-12 alternative
Grade Microfluidics Suitable (0.04 µm final) Not graded Not graded
Base Formulation DMEM/F-12 (1:1), 15 mM HEPES, bicarbonate-free DMEM/F-12 Standard DMEM/F-12 Equivalent
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 stages 1 stage 1–2 stages
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP particulate compliance check_circle USP <788> Method 1 cancel cancel
Water quality Ultrapure Type 1 (18.2 MΩ·cm) Purified water Purified water
Manufacturing QMS ISO 13485:2016 Variable Variable
Microfluidic channel compatibility check_circle Validated cancel Higher clogging risk cancel Higher clogging risk
Custom formulation check_circle On request cancel Limited

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Dulbecco’s Modified Eagle Medium/ Ham's F-12 Nutrient Mixture (DMEM/F-12) (1:1) with 15mM HEPES w/o Sodium Bicarbonate: 1X Liquid and Microfluidics Suitable cell culture media.

Yes. DCP-DM12H-BT1X uses our Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2), a Microfluidics Suitable formulation delivering ultra-low particulate counts that reduce the risk of microchannel clogging in OoC and MPS devices.
Standard 0.22 µm filtration leaves intact mycoplasma-sized particulates (0.2–0.3 µm) and subvisible debris that can accumulate in microchannels. FluxMPS™ uses four sequential stages reaching 0.04 µm, delivering approximately 5× lower particulate counts per USP <788> Method 1.
This formulation uses 15 mM HEPES (3574.5 mg/L) as the primary buffer instead of sodium bicarbonate, giving greater pH stability outside a CO₂ incubator — useful for microfluidic and open-bench workflows. It already contains glucose (3151 mg/L), L-glutamine (365 mg/L), and sodium pyruvate (110 mg/L); no additional carbon source is required for most standard cell lines. Contact support@diagnocine.com for custom concentrations.
No bicarbonate is present; this formulation is HEPES-buffered, reducing CO₂ dependence. It can typically be used with standard incubation, though CO₂ requirements should be validated per cell line.
Yes. This medium can be supplemented with FBS (5–20%), growth factors, antibiotics, or other additives per standard practice. Add supplements immediately before use. If filtering serum or protein-containing supplements, use a 0.2 µm low-protein-binding PES or PVDF filter; do not use a 0.04 µm filter, which retains IgM, lipoproteins, and much of serum's functional content.
Endotoxin is controlled per manufacturing batch by LAL assay per USP <85> BET (assay sensitivity 0.005 EU/mL). Release specification: < 0.05 EU/mL. Batch-specific results are in the Certificate of Analysis, available from support@diagnocine.com.
Yes. A batch-specific CoA is available for every shipment and includes: appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85> BET), sterility (USP <71>), particulate matter (USP <788> Method 1), raw material traceability, manufacturing date, and authorized release signatures. Request via support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed publications supporting Microfluidics Suitable, ultra-filtered media and microfluidic cell culture applications.

  1. Huh D et al. (2010). Reconstituting organ-level lung functions on a chip. Science, 328(5986), 1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN & Ingber DE (2014). Microfluidic organs-on-chips. Nature Biotechnology, 32(8), 760–772. doi:10.1038/nbt.2989
  3. Eagle H (1959). Amino acid metabolism in mammalian cell cultures. Science, 130(3373), 432–437. doi:10.1126/science.130.3373.432
  4. Bhattacharya S et al. (2018). Challenges in maintaining cell viability during microfluidic experiments. Electrophoresis, 39(7), 997–1006. doi:10.1002/elps.201700375
  5. Zhang YS et al. (2017). Multisensor-integrated organs-on-chips platform for automated in situ monitoring. PNAS, 114(12), E2293–E2302. doi:10.1073/pnas.1612906114
  6. Vernetti L et al. (2017). Functional coupling of human microphysiology systems. Scientific Reports, 7, 42296. doi:10.1038/srep42296
  7. Esch EW et al. (2015). Organs-on-chips at the frontiers of drug discovery. Nature Reviews Drug Discovery, 14(4), 248–260. doi:10.1038/nrd4539
  8. Zheng F et al. (2021). Organ-on-a-chip systems: microengineering to biomimic living systems. Small, 17(7), 2004175. doi:10.1002/smll.202004175

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