DNA polymerase I (E. coli)
Cat. No.: SB-G3478-500U
Size: 500U
Description
DNA polymerase I ( E.coli ) is a DNA polymerase I derived from Escherichia coli and expressed recombinantly by Escherichia coli. The 5'-3' polymerase activity of DNA polymerase I is retained. In the presence of templates and primers (DNA or RNA), dNTPs are used as substrates to synthesize DNA complementary to the template along the 5'-3' direction. The enzyme also retains double-strand specific 5'-3' exonuclease activity and single-strand specific 3'-5' exonuclease activity.
- Source : derived from Escherichia coli, recombinantly expressed by Escherichia coli.
- Definition of enzyme activity: at 37°C, the amount of enzyme required to catalyze the incorporation of 10 nmol deoxyribonucleotides into polynucleotides within 30 minutes is defined as one enzyme activity unit.
- Purity and concentration: SDS-PAGE detection purity ≥ 95%; endogenous nucleic acid residue < 1 pg/μL (qPCR detection); 5 U/μL.
- Inactivation or inhibition: Heating at 75°C for 20 min or adding an appropriate amount of EDTA can lead to enzyme inactivation. Metal ion chelating agents and inorganic pyrophosphates can inhibit enzymes.
- Enzyme Storage Buffer: 25 mM Tris-HCl, 1 mM DTT, 0.1 mM EDTA, 50% Glycerol, pH 7.4.
- 10x Reaction Buffer: 100 mM Tris-HCl, 500 mM NaCl, 100 mM MgCl2 , 10 mM DTT, pH7.9.
Storage and transportation
Transport in wet ice packs; store at -20°C, valid for 12 months.
Product Contents
| Component Number |
Component |
G3478 |
| G3478-1 |
DNA polymerase I ( E. coli ) |
100 μL |
| G3478-2 |
10x Reaction buffer |
500 μL |
| Product Manual |
1 copy |
Precautions
1. All enzymes should be stored in an ice box and stored at -20°C immediately after use.
2. For your safety and health, please wear a lab coat and disposable gloves for operation.
