Anti-Rad6 antibody, affinity purified (rabbit polyclonal)

Product#: 70-020
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Anti-Rad6 antibody, affinity purified (rabbit polyclonal)

General information 

Cat. No. 70-020
Size :100 ug
Antigen Species Human
Host Species Rabbit
Purification Affinity purified with the immunogen conjugated to agarose
Reactivity Reacts with Rad6A and Rad6B of human mouse, rat and hamster. Not tested in other species
Immunogen Human Rad6B full-length recombinant protein
Application   :1. Western blotting (1,000~2,000 fold dilution)
:2. Immunoprecipitation (assay dependent)
:3. Immunofluoscent staining (assay dependent)
:4. Other applications have not been tested
Storage Shipped at 4℃. Upon arrival, spin-down and store at -20℃.
Form 1mg/ml in PBS, 50% glycerol. Filter-sterilyzed. Azide- and carrier-free
Data Link UniProtKB/Swiss P49459 (UBE2A/Rad6A_HUMAN), UniProtKB/Swiss P63146 (UBE2B/Rad6B_HUMAN)


Resume of DNA synthesis, which was halted by various physical and chemical factors, was triggered by repair system including Rad6. Rad6 (152a.a, 17.3kDa) is the ubiqutin-conjugated enzyme E2A, and complex with two Rad18 molecules, ubiquitin ligase E3, to ubiquitinate PCNA by utilizing poly-ubiquitin chain that HLTF-UBC13 synthesized and they are involved in translesion DNA synthesis (TLS) and template switch (TS). Two isoforms of Rad6, Rad6A and Rad6B differs in only 6 amino acids. They also play an important role in epigenetic transcriptional regulation by catalyzing the monoubiquitination of histone H2B at Lys-120 .

Anti-Rad6-1.png Anti-Rad6-2.png
Fig.1 Western blot analysis of Rad6 in the whole cell extracts of Hela cell (10 ug) with anti-Rad6
Fig. 2 Rad6 protein in GM637 cells were stained by indirect immunostaining method with the Rad6 antibody.


  1. Masuyama S. et al. “Regulated expression and dynamic changes in subnuclear localization of mammalian Rad18 under normal and genotoxic conditions” Genes to Cells 10: 753-762 (2005) PubMed 16098139 WB, IF

Aliases for UBE2A Gene

  • Ubiquitin Conjugating Enzyme E2 A 2 3 5
  • HHR6A 2 3 4
  • RAD6A 2 3 4
  • Ubiquitin-Conjugating Enzyme E2 A 3 4
  • E2 Ubiquitin-Conjugating Enzyme A 3 4
  • Ubiquitin-Conjugating Enzyme E2A 2 3
  • Ubiquitin Carrier Protein A 3 4
  • Ubiquitin-Protein Ligase A 3 4
  • RAD6 Homolog A 3 4
  • UBC2 2 3
  • HR6A 2 4
  • Ubiquitin-Conjugating Enzyme E2A (RAD6 Homolog) 2
  • EC 51
  • EC 4
  • MRXS30 3
  • MRXSN 3
  • UBE2A 5

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