Xyltech Growth MSC Supplement AF(#10413)

Cat. No.: FNK-87-333

Size: 10ml

Storage: -20℃


Product features:

This product is a culture medium suitable for the growth of human mesenchymal stem cells (hMSCs). When used in combination with Xyltech™ MSC-01 Xeno-Free or Xyltech™ MSC-02 Animal-Free, hMSCs proliferation can be controlled as needed. The basal medium “Xyltech™ Growth MSC Medium” should be used with the additive “Xyltech™ Growth MSC Supplement XF” or “Xyltech™ Growth MSC Supplement AF”. In addition, there is no need to pre-coat culture vessels.

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Product Name	CAT. NO.	Volume	Note	Shop Now
Xyltech™ Growth MSC Medium	FNK-87-331	500 mL	Basal medium (*Animal-Free)
Xyltech™ Growth MSC Supplement XF	FNK-87-332	10 mL	**Xeno-Free supplement
Xyltech™ Growth MSC Supplement AF	FNK-87-333	10 mL	*Animal-Free supplemen	Current item

*Animal-Free: It contains no ingredients derived from animals.
**Xeno-Free: It contains human-derived components and is free from any other animal-derived ingredients.

Precautions for use

Xyltech™ Growth MSC does not contain substances that neutralize trypsin activity. When subculturing cells with trypsin, it is strongly recommended that the trypsin activity be sufficiently neutralized with a trypsin inhibitor. Dilution washing alone does not completely remove trypsin activity and the remaining protease activity will reduce subsequent cell growth.

This product is a research reagent. It cannot be used for human or animal treatment or diagnostic purposes.

Thawing and preparation

Quickly thaw a frozen supplement in a 37°C water bath, being careful not to submerge the entire bottle. Watch the supplement closely; when the whole contents melt, please take it out immediately from the water bath. Once thawed, the supplement should be stored at 2-8°C and added to the basal medium within 2 weeks. After the supplement is added to the basal medium, use it immediately.

It is recommended to prepare the required volume of the medium just before use. For small volume preparation, add 1/50 volume of Xyltech™ Growth MSC Supplement XF or Xyltech™ Growth MSC Supplement AF to Xyltech™ Growth MSC Medium. Additionally, do not freeze the medium after adding the supplement to avoid deterioration of some active ingredients.

Fine particles may be observed but there is no influence on quality.


Example of cell culture protocol for normal human adipose-derived stem cells (ADSCs) culture using Xyltech™ Growth MSC

Required cells and reagents:

• Normal human ADSCs (100 mm-dish)
• Xyltech™ Growth MSC Medium (Cat. No. FNK-87-331)
• Xyltech™ Growth MSC Supplement XF (Cat. No. FNK-87-332)
• Xyltech™ Growth MSC Supplement AF (Cat. No. FNK-87-333)
• r-TE (Recombinant Trypsin/EDTA Solution) (Cat. No. FNK-87-974)
• s-TI (Synthetic Trypsin Inhibitor Solution) (Cat. No. FNK-87-975)
• D-PBS (-)

Cell culture of normal human ADSCs

1. Before using Xyltech™ Growth MSC, add the entire amount of Xyltech™ Growth MSC Supplement XF or Xyltech™ Growth MSC Supplement AF (thawed before use) to Xyltech™ Growth MSC Medium and mix well.
2. Warm the Xyltech™ Growth MSC, D-PBS (-), r-TE, and s-TI in a 37°C water bath.
3. Remove the culture supernatant of selected normal human ADSCs that have reached approximately 80% confluence (sub confluence).
4. Rinse the cell layer with 5 mL of D-PBS (-).
5. Add 0.5 mL of r-TE and incubate at 37°C for approximately 2 minutes.
6. Add 0.5 mL of s-TI, mix well, gently pipette up and down several times, collect cells from the dish, and centrifuge at 1,000 rpm, for 5 minutes.
7. Aspirate the supernatant and add the appropriate amount of Xyltech™ Growth MSC to resuspend the cells and seed into a new tissue culture dish.
8. The cells will become confluent within 2-4 days when cultured in Xyltech™ Growth MSC. Start subculture and/or experiments with the cells.
9. For experiments requiring a reduced growth rate, the cells can be cultured for 3 days in Xyltech™ MSC-01 Xeno-Free or Xyltech™ MSC-02 Animal-Free growth suppressive medium (refer to protocol for suppressive growth control using Xyltech™ MSC-01 Xeno-Free, FNK-87-330 or Xyltech™ MSC-02 Animal-Free, FNK-87-334).