Cell Tissue RNA Mini Kit (50 Preps)
Cat. No.: BMG-R6311-01
Size: 50 Preps
Description
The EZgeneTM Tissue RNA kit provides an easy and fast method for isolating total RNA from tissues, cultured cells within 30 min. Only trace genomic DNA exists in the purified RNA, which can be eliminated by DNase I treatment (See detail in the protocol) when it is necessary.
Storage and Stability
All components can be stored at room temperature. All kit components are guaranteed for 1 year from the date of purchasing.
Kit Contents
| Catalog# | BMG-R6311-01 | BMG-R6311-02 |
| Buffer LY | 30 mL | 135 mL |
| Buffer RB | 30 mL | 135 mL |
| RNA Wash Buffer | 12 mL | 50 mL |
| DEPC-Treated ddH2O | 10 mL | 30 mL |
| RNA Columns | 50 | 250 |
| User Menu | 1 | 1 |
Important
- Add 1% volume of ß-mercaptoethanol to Buffer LY before use and store at 4°C.
- Add 48 mL (R6311-01) or 200 mL (R6311-02) 100% ethanol to RNA Wash Buffer before use.
Table I: Recommend amounts of cells or tissues per preparation
| Sample | Max Tissue or Cell Mass per prep | Total RNA Yield (ug) |
| Liver | 30 mg | 130 |
| Kidney | 20 mg | 45 |
| Muscle | 80 mg | 50 |
| Spleen | 15 mg | 80 |
| Heart | 100 mg | 50 |
| Brain | 120 mg | 80 |
| Lung | 100 mg | 70 |
| Pancreas | 30 mg | 100 |
| Tomato Leaves | 200 mg | 30 |
| HeLa Cells | 5x106 | 120 |
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