The EZgeneTM Tissue RNA Kit plus combines the reversible binding properties of HiBind RNA technology with a specially designed buffer system which can effectively remove DNA before RNA isolation. Samples are first lysed and homogenized in a specially designed denaturing buffer, which immediately inhibits the activity of RNase. The lysate is then passed through a EZgeneTM DNA Clearance column which traps the genomic DNA. The flow-through lysate is then applied to RNA column to bind RNA. After two wash steps, purified RNA is eluted with RNase-free water. The whole protocol can be completed in less than 30 minutes. RNA purified is ready for applications such as NGS, RT-PCR, qPCR, differential display, and microarrays.
Storage and Stability
All components can be stored at room temperature. All kit components are guaranteed for 1 year from the date of purchasing.
Kit Contents
Catalog#
BMG-R6321-00
BMG-R6321-01
BMG-R6321-02
Preps
4
50
250
Buffer HLY
4 mL
40 mL
150 mL
Buffer HRB
4 mL
50 mL
150 mL
RNA Wash Buffer
2 mL
12 mL
50 mL
DEPC-Treated ddH2O
1.0 mL
10 mL
30 mL
ezBind RNA Columns
4
50
250
DNA Clearance Column
4
50
250
User Menu
1
1
1
Important
Calculate and aliquot amount of Buffer HLY to be used in a clean tube and add 10 µL βmercaptoethanol (β-Me) or 40 μL DTT (1 M) to 1 mL Buffer HLY. Add 4 µL of L Solution per 1 mL of Buffer HLY/β-Me or Buffer HLY/DTT. Buffer HLY contains β-Me or DTT can be stored at room temperature up to 1 month.
Crystals may form in Buffer HLY, dissolve the precipitates at 37°C before use.
Add 8 mL (R6321-00) or 48 mL (R6321-01) or 200 mL (R6321-02) 100% ethanol to RNA Wash Buffer before use. The final ethanol is 80% (v/v).
Materials supplied by users
Tabletop microcentrifuge.
Sterile RNase free 1.5 mL centrifuge tubes and tips.
Vacuum manifold if use vacuum protocol.
14.3 M -mercaptoethanol.
Safety information
Buffer HLY contains chaotropic salts, which may form reactive compounds when combines with bleach. Do not add bleach or acidic solutions directly to the preparation waste, wear gloves and protective eyewear when handling.
Before starting
Prepare all components and get all necessary materials ready by examining this instruction booklet and become familiar with each steps.
