The EZgeneTM 96-well plasmid kit provides an easy and fast method for isolating high quality plasmid DNA in a high through put format. The key to this system is Biomiga’s ezbind matrix that avidly, but reversibly, binds DNA under optimized buffer condition while proteins and other unwanted contaminants are removed by wash buffer. High quality plasmid DNAs are eluted with TE buffer or deionized water. By using the 96-well kit, up to 96 samples can be simultaneously processed in less than 90 minutes. The lysate clearance plate obviates the time-consuming centrifugation step and increases the DNA recovery up to 20%.
Note: The yield of plasmid DNA depends on the origin of the replication and the size of the plasmid. The protocols are optimized for high copy number plasmid purification. For low copy number plasmids, both the culture volume and the buffer volume need to be scaled up 3 to 5 times. Please contact our customer service for further information and reference the table below for the commonly used plasmids,
Storage and Stability
All components are guaranteed for 24 months from the date of purchase. The Buffer A1/RNase A should be stored at 4°C. Buffer B1 needs to be tightly capped to avoid oxidation.
Kit Contents
Catalog#
BMG-PD1812-00
BMG-PD1812-1
BMG-PD1812-2
Components
1x96 Preps
4x96 Preps
20x96 Preps
DNA plate
1
4
20
Lysate clearance plate
1
4
20
Buffer A1
60 mL
120 mL
600 mL
Buffer B1
60 mL
120 mL
600 mL
Buffer N1
80 mL
160 mL
750 mL
DNA Wash Buffer
50 mL
3 x 50mL
5 x 200mL
Buffer KB
60 mL
80 mL
1000 mL
Elution buffer
30 mL
70 mL
500 mL
RNase A
200 µL
400 µL
2000 µL
Breathable films
1
4
20
Sealing Films
4
16
80
Caution: Buffer N1 and Buffer KB contain chaotropic salts, wear gloves and protective eyewear when handling.
