dsDNase (Thermolabile)
Cat. No.: SB-G3408-50UL
Size: 50UL
Description
The Servicebio dsDNase (thermolabile) is a heat-sensitive double chain deoxyribonuclease, which is recombined and expressed by Pichia pastoris and derived from shrimp. The double stranded deoxyribonuclease specifically cleaves the phosphate diester bond in double stranded DNA to produce oligonucleotides with 5 ' -phosphate and 3 ' -hydroxyl ends. This product has strong specificity, does not degrade RNA and single stranded DNA, and is sensitive to heat. It can be inactivated after treatment at 55 ℃ for 5 minutes. These characteristics are very suitable for quickly and safely removing genomic DNA contamination in RNA samples before reverse transcription, simplifying the workflow, and making genomic DNA clearance and cDNA synthesis in a single tube.
- Source : Shremp derived deoxyribonuclease, recombinant expression of Pichia pastoris.
- Definition of enzyme activity : Under the condition of 25 ℃ and pH 5.0, the enzyme amount required to increase the reaction system A260 by 0.001 per minute with double chain DNA as substrate is defined as an enzyme activity unit.
- Purity : SDS-PAGE detection purity ≥ 95%.
- Inactivation or inhibition : treatment at 55 ℃ for 5 min can completely inactivate dsDNase (thermolabile); High activity of metal ions, chelating agents, SDS and reducing agents can inhibit the enzyme activity of dsDNase (thermolabile).
- Enzyme storage buffer : 25 mM Tris-HCl, 2 mM MgCl2 , 10 mM NaCl, 0.01% Triton X-100, 50% glycerol, pH 7.5 .
- 10 × Reaction Buffer : 200 mM Tris-HCl, 60 mM MgCl2 , pH 8.3 .
Storage and Handling Conditions
- Storage conditions: -20 ℃ .
- Shipping conditions: Wet ice.
- Shelf life: 12 months from date of manufacture .
Composition
| Component Number |
Component |
G3408-50UL |
| G3408-1 |
dsDNase (Thermolabile) |
50 μL |
| G3408-2 |
10 × Reaction Buffers |
100 μL |
| Product Manual |
1 copy |
Assay Protocol / Procedures
1. Recommend reaction system:
| Component |
Volume |
| dsDNase (Thermolabile) |
1 μL |
| 10 × Reaction Buffers |
1 μL |
| RNA samples |
Up to 8 μL |
| Nuclease Free Water |
To 10 μL |
After adding samples according to the above reaction system, gently mix them and incubate them at 37 ℃ for about 2 min, which can effectively remove the double stranded DNA pollution in RNA samples
2. If the treated RNA sample is subsequently used for reverse transcription, the system must be heated at 55 ℃ for 5 min to fully inactivate ds DNase (thermolabile).
Note:
- The enzyme products should be put in the ice box or on the ice when in use. After use, they should be stored at - 20 ℃ immediately. It is recommended to store them separately
- For your safety and health, please wear lab clothes and disposable gloves .
