TBS, 10X (Tris Buffered Saline), Non-Sterile

FluxMPS™ TBS, 10X (Tris Buffered Saline), Non-Sterile

An MPS-grade, 10X concentrated Tris Buffered Saline formulated with 200 mM Tris and 1500 mM NaCl at pH 7.4, engineered for defined, reproducible buffering across washing, dilution, and immunoassay workflows. Quadruple-stage 0.1 µm / 0.04 µm membrane filtration and mycoplasma-free release testing support low-background performance in microfluidic, organ-on-a-chip, and molecular biology applications. Supplied non-sterile for research use, with pH, concentration, and additive customization available on request.

• Quadruple-stage filtration: 0.1 µm membrane filtration (twice) plus 0.04 µm membrane filtration (twice)
• Mycoplasma-free (no mycoplasma detected)
• Precise pH 7.4; 10X concentrate with 1X, 2X, 4X, and 5X customization available
• Defined formulation: 200 mM Tris and 1500 mM NaCl
• ASTM Type 1 Ultrapure Water (0.05 µS/cm), deionized
• Protease(-), DNase(-), RNase(-), Endonuclease(-)
• Non-sterile, RUO grade; Tissue Culture & Molecular Biology grade
• Custom pH, concentration, and additive/detergent/inhibitor/protein formulations available on request

Engineered where standard TBS falls short

Tris-buffered saline (TBS) is an isotonic, non-toxic buffer used to maintain a slightly alkaline pH range of 7–9 in a wide range of biochemical workflows. Tris acts as the buffering agent while NaCl regulates tonicity, and the conjugate acid of Tris carries a pKa of 8.07 at 25°C, with solution pH shifting approximately 0.03 units per degree Celsius as temperature changes. Conventional 0.22 µm-filtered buffer can carry subvisible particulates, inconsistent salt lots, and uncontrolled bioburden into sensitive workflows — FluxMPS™ TBS, 10X is built to remove those variables.

Quadruple-stage filtration system

Every batch of FluxMPS™ TBS, 10X is filtered 0.1-micron membrane twice and 0.04-micron membrane twice, sequentially removing particulate and bioburden ahead of final release testing that confirms no mycoplasma is present.

1. 1

   0.1 µm Pre-filtration I

   First-pass removal of large particulates and aggregates, extending the working life of downstream filters.

2. 2

   0.04 µm Pre-filtration II

   Retention of finer particulates and bioburden, including organisms in the size range relevant to mycoplasma screening.

3. 3

   0.1 µm Filtration II

   Second 0.1 µm pass, providing redundancy ahead of the final polishing stage.

4. 4

   0.04 µm Filtration II — Final Polish

   Second 0.04 µm pass for final polish prior to fill, supporting the product's mycoplasma-free release status.

Where FluxMPS™ TBS, 10X is used

As a laboratory reagent, TBS is commonly used for washing, diluting, cell culture processing, Western blotting, ELISA, and multiple assays and immunohistochemistry steps, with tonicity governed by NaCl and pH governed by the Tris buffering system.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) ultra-filtered variant is available for automated bioreactor and robotic liquid-handling platforms where valve and sensor protection is critical.

• Total Particulate Exclusion for automated fluid paths
• Valve & Sensor Protection in closed perfusion systems
• Extended Perfusion Stability for long-duration runs

Inquiry Required: Contact support@diagnocine.com to request the 0.01 µm ultra-filtered grade of DCP-TBS10X.

Full specification sheet

Measured and declared values for FluxMPS™ TBS, 10X (DCP-TBS10X), as supplied.

Full composition

Component concentrations for the 10X concentrate, as formulated per lot.

Manufacturing & compliance

FluxMPS™ TBS, 10X is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision), with final packaging, quality assurance, and testing performed at the DiagnoCine R&D and Quality Testing Center.

How DCP-TBS10X compares

FluxMPS™ TBS, 10X against conventional TBS preparations.

Frequently asked questions

Common questions about FluxMPS™ TBS, 10X (DCP-TBS10X).

Supporting literature

Curated references relevant to Tris-buffered saline chemistry and its laboratory applications.

1. Good NE, et al. Hydrogen ion buffers for biological research. Biochemistry. 1966. doi:10.1021/bi00866a011
2. Ferguson WJ, et al. Hydrogen ion buffers for biological research. Anal Biochem. 1980. doi:10.1016/0003-2697(80)90269-1
3. Towbin H, et al. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets. Proc Natl Acad Sci USA. 1979. doi:10.1073/pnas.76.9.4350
4. Engvall E, Perlmann P. Enzyme-linked immunosorbent assay (ELISA). Immunochemistry. 1971. doi:10.1016/0019-2791(71)90454-X
5. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014. doi:10.1038/nbt.2989
6. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010. doi:10.1126/science.1188302
7. Rasooly A, Herold KE. Biosensors for the analysis of food- and waterborne pathogens and their toxins. J AOAC Int. 2006. doi:10.1093/jaoac/89.3.873
8. Uphoff CC, Drexler HG. Detection of mycoplasma contamination in cell cultures. Curr Protoc Mol Biol. 2014. doi:10.1002/0471142727.mb2804s106
9. Ramsden JJ. Ionic strength effects on protein and cellular interactions. Colloids Surf B Biointerfaces. 1999. doi:10.1016/S0927-7765(99)00104-1
10. Malda J, et al. Of mice, men and elephants: the relation between endotoxin biosensing and biomaterials. Trends Biotechnol. 2013. doi:10.1016/j.tibtech.2013.06.002