UltraClean PVSA-Supplemented SSC Buffer, 1X (Saline Sodium Citrate), RNase-Safe
Cat No.:
DCP-SSCPVSA1X
Size: 500 mL and 1000 mL
Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane TWICE.
- World’s cleanest RNase-safe SSC buffer for RNA handling, cell, and molecular biology experiments
Description:
PVSA-Supplemented SSC Buffer, 1X is a ready-to-use, high-purity saline sodium citrate buffer formulated to be RNase-safe for demanding RNA and DNA workflows. It is supplemented with polyvinylsulfonic acid (PVSA), a potent polymeric RNase inhibitor that helps preserve RNA integrity during sample handling, hybridization, washing, and short-term storage. The buffer provides the ionic strength and salt composition characteristic of 1X SSC, making it suitable for use with nucleic acid probes, RNA-containing samples, and cells in sensitive downstream applications.
Applications:
- RNA Hybridization and Wash: Hybridization and wash buffer for Northern blotting, in situ hybridization, and RNA probe-based assays where RNase control is critical.
- Nucleic Acid Blotting and Microarrays: Wash and incubation buffer for Southern and Northern blots and DNA/RNA microarrays performed under SSC conditions.
- RNA Handling and Short-Term Storage: Washing, resuspension, and temporary storage of RNA-containing samples, cells, or tissues in high-salt buffer with added RNase protection.
- Probe-Based Assays and RNA Capture: Dilution of RNA probes, oligos, and conjugates, and washing of solid supports in workflows requiring strict nuclease control in SSC buffer.
- General Molecular Biology: Any procedure requiring 1X SSC with enhanced RNA protection, including high-salt washes and handling steps for RNA-sensitive samples.
Advantages:
- RNase-Safe: PVSA provides robust inhibition of a wide range of RNases, helping protect RNA without relying solely on protein-based inhibitors.
- High Purity: Manufactured from molecular biology-grade components and subjected to multiple fine-pore filtration steps to minimize particulates, microbes, and adventitious nucleases.
- Optimized Ionic Strength: Formulated at standard 1X SSC ionic strength to support nucleic acid hybridization, stringency washes, and probe stability.
- Convenient Hybridization Buffer: SSC-based formulation is directly compatible with many established hybridization and wash protocols for RNA and DNA.
- Ready-to-Use 1X Solution: No dilution or mixing required, minimizing preparation time and contamination risk.
Composition:
| Ingredients |
Concentration |
| Sodium Chloride |
150 mM |
| Sodium Citrate |
15 mM |
| PVSA (Polyvinylsulfonic Acid) |
1g/L |
| Water (Ultrapure, nuclease-free) |
To volume |
Quality Control:
Storage and Shelf Life:
-
Store at 2–8°C.
-
Do not freeze.
-
Shelf life: 12 months.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration and 0.04-micron filtration twice. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
