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FluxMPS™ MCDB 151 Medium w/o Sodium Bicarbonate: 1X Liquid

Product#: DCP-M151H-B1X
$71.49
DCP-M151H-B1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ MCDB 151 Medium w/o Sodium Bicarbonate: 1X Liquid

Contains L-Glutamine Contains Phenol Red Contains HEPES (28 mM) Contains Calcium Contains Magnesium Contains Glucose (1.081 g/L) Contains Sodium Pyruvate Without Sodium Bicarbonate

FluxMPS™ DCP-M151H-B1X is a Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) MCDB 151 −Bicarbonate +HEPES formulation engineered for serum-free or low-protein culture of Human Epidermal Keratinocytes and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. The train reaches a 0.04 µm final cut-off — five times finer than the 0.22 µm membranes used for conventional filtration — so trace element salts are delivered fully dissolved rather than as particulate aggregates. Formulation: [+] L-Glutamine, [+] Phenol Red, [+] HEPES (28 mM), [+] Calcium, [+] Magnesium, [+] Glucose (1.081 g/L), [+] Sodium Pyruvate | [-] Sodium Bicarbonate.

  • Trace element formulation — zinc, copper and iron for metalloenzyme support and antioxidant activity in serum-free culture
  • Optimized for Human Epidermal Keratinocytes and CHO cells in CO2-independent culture systems
  • 28 mM HEPES (pKa 7.3 at 37°C) — pH-stable without CO2 for open-top chips and atmospheric platforms
  • Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off — 5.5× finer than 0.22 µm conventional filtration by pore size
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
  • Formulated without sodium bicarbonate; buffering provided entirely by HEPES
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Custom pH, glucose, trace element and growth-factor co-formulations available on request
CAT. NO.
DCP-M151H-B1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
MCDB 151 Medium w/o Sodium Bicarbonate: 1X Liquid
  • Cell typesHuman Epidermal Keratinocytes
  • Glucose1081 mg/L (1.081 g/L)
  • HEPES28 mM, pKa 7.3 at 37°C
  • Sodium Pyruvate55 mg/L
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)290–330 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

MCDB media formulated with trace element salts can form subvisible particulate aggregates when filtered at 0.22 µm under typical manufacturing conditions. These aggregates clog microfluidic channels, disrupt trace metal bioavailability, and add particulate background to imaging and biosensor readouts. FluxMPS™ addresses these failure modes with four-stage sub-0.04 µm filtration and a < 0.05 EU/mL endotoxin release specification.

filter_alt

Microchannel-safe purity

0.04 µm final filtration keeps trace metal salts fully dissolved — no aggregate deposition in chip channels or culture vessels, and compliance with USP <788> particulate limits.

target

Total metabolic control

A defined glucose, sodium pyruvate and HEPES composition — without added sodium bicarbonate — supports controlled, CO2-independent culture conditions for keratinocyte and CHO cell studies.

water_drop

Ultrapure-grade water

Ultrapure Type 1 water (18.2 MΩ·cm) reduces trace-metal and organic-carbon background that would otherwise compete with zinc, copper and iron uptake by low-serum primary cells.

visibility

Low background for imaging

Ultra-low particulate filtration reduces debris background for confocal microscopy and biosensor-based readouts in chip-based assays. This formulation contains phenol red for visual pH monitoring.

science

Rich, stable nutrient profile

20 amino acids, 10 vitamins, and a defined trace element matrix (zinc, copper, iron) — released per micro-batch with full Certificate of Analysis traceability.

tune

Customization on demand

pH, glucose, trace element concentrations, HEPES, and growth factor co-formulations available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages — a repeated pre-filter + final-filter pair run twice — reach a final 0.04 µm polish, delivering particle-free trace element delivery unavailable from conventional 0.22 µm filtered MCDB media.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates including trace metal salt precipitates; protects the first 0.04 µm cartridge from fouling.

  2. 2

    0.04 µm Final Filtration I — Mycoplasma Barrier

    Retains mycoplasma-sized organisms (0.2–0.3 µm) and residual fine particulates absent from standard 0.22 µm filtration.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated prefilter protecting the second 0.04 µm cartridge — redundant protection, not a re-filtration of the first pass.

  4. 4

    0.04 µm Final Filtration II — Polish

    Ultimate polishing filter prior to aseptic fill & finish.

Filtration architecture vs. conventional media

A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration of MCDB 151 media.

5.5×
Finer than 0.22 µm conventional filtration, by pore size
4
Filtration passes to a 0.04 µm final pore size
Sterility: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved through 0.1 µm mycoplasma-retentive filtration; this is a filtration statement, not a per-lot USP <63> test result.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-M151H-B1X MCDB 151 Medium w/o Sodium Bicarbonate: 1X Liquid ? Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final Filtration I, 0.1 micron Prefiltration II, 0.04 micron Final Filtration II Polish ? Microfluidics Suitable MCDB 151 minus Bicarbonate plus HEPES for organ-on-a-chip and microphysiological system applications | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) ensuring particle-free trace element delivery.
© Diagnocine® — DCP-M151H-B1X
Applications

Specialized cell models & OoC applications

FluxMPS™ DCP-M151H-B1X was formulated for Human Epidermal Keratinocytes and CHO cells in low-protein or serum-free, CO2-independent conditions. The 0.04 µm filtered trace element matrix supports safe use in microfluidic chip architectures where conventional MCDB media can cause trace metal particulate fouling.

Automated Bioreactors & Robotics

Next-Generation System Uptime

An optional 0.01 µm (10 nm) ultra-filtered variant of this MCDB 151 formulation — the separate MPS Grade line described above — is available for automated bioreactor perfusion where trace metal salt nanoparticulates can cause valve fouling.

  • Trace Metal Nanoparticulate Removal: 10 nm filtration targets sub-0.04 µm metal salt colloids not addressed by standard QC
  • Valve & Sensor Protection: Reduces micro-fouling risk from trace element aggregates in automated systems
  • Extended Perfusion Stability: Supports consistent trace element delivery over weeks-long primary cell culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Microfluidics

Organ-on-a-Chip & MPS

Ultra-clean 0.04 µm filtration reduces the risk of microchannel clogging. Trace elements are delivered particle-free — no aggregate deposition in chip channels.

OoCToCMPS
Dermal Biology

Skin-on-Chip

FluxMPS™ MCDB 151 −Bicarbonate +HEPES supports sub-mycoplasma-filtration purity and trace element fidelity for skin-on-chip models cultured under low-protein or serum-free conditions.

Primary keratinocytesSkin barrier models
Epithelial Biology

Keratinocyte OoC

HEPES-buffered, bicarbonate-free formulation supports stable pH during static and perfused keratinocyte chip culture without CO2 supplementation.

Human Epidermal Keratinocytes
Bioprocessing

CO2-Independent CHO Culture

Bicarbonate-free HEPES buffering enables CHO cell culture in open or CO2-limited systems, including certain robotic and automated culture platforms.

CHO cells
Assay Development

Open-Top Epidermal Chips

Stable HEPES buffering supports extended, gas-exchange-limited incubation typical of open-top epidermal and transwell barrier chip designs.

Transwell barrier assaysTEER
Live-Cell Imaging

Imaging & Biosensors

Low particulate background supports confocal microscopy and biosensor-based readouts in chip-based assays. Phenol red is present in this formulation for visual pH monitoring.

ConfocalBiosensorsTEER
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. Available sizes: 500 mL, 1000 mL. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] L-Glutamine, [+] Phenol Red, [+] HEPES (28 mM), [+] Calcium, [+] Magnesium, [+] Glucose (1.081 g/L), [+] Sodium Pyruvate | [-] Sodium Bicarbonate
Appearance Red-colored, clear solution (phenol red present)
Glucose 1081 mg/L (1.081 g/L)
HEPES 28 mM (pKa 7.3 at 37°C)
Sodium Pyruvate 55 mg/L
pH USP <791> 7.4
Osmolality USP <785> 290–330 mOsm/kg H2O
Total ingredients 47 components across 4 categories
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification; see Manufacturing & Compliance)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent — 28 mM HEPES maintains pH without gas supplementation
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

MCDB 151 −Bicarbonate +HEPES: 47 ingredients verified per lot with CAS numbers for raw-material traceability. Contains trace elements (zinc, copper, iron) for metalloenzyme support and antioxidant activity. HEPES (28 mM, 6600 mg/L) is listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 4.411
Cupric sulfate pentahydrate 7758-99-8 0.0025
Disodium hydrogen phosphate anhydrous 7558-79-4 284.080
Ferrous sulfate heptahydrate 7782-63-0 0.417
Magnesium chloride hexahydrate 7791-18-6 122.000
Potassium chloride 7447-40-7 111.830
Sodium acetate anhydrous 127-09-3 301.530
Sodium chloride 7647-14-5 7599.000
Zinc sulfate heptahydrate 7446-20-0 0.863
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 7.510
L-Alanine 56-41-7 8.910
L-Arginine hydrochloride 1119-34-2 210.700
L-Asparagine monohydrate 5794-13-8 15.000
L-Aspartic acid 56-84-8 3.990
L-Cysteine hydrochloride monohydrate 7048-04-6 42.040
L-Glutamic acid 56-86-0 14.710
L-Glutamine 56-85-9 877.200
L-Histidine hydrochloride monohydrate 5934-29-2 16.770
L-Isoleucine 73-32-5 1.968
L-Leucine 61-90-5 65.600
L-Lysine hydrochloride 657-27-2 18.270
L-Methionine 63-68-3 4.476
L-Phenylalanine 63-91-2 4.956
L-Proline 147-85-3 34.530
L-Serine 56-45-1 63.060
L-Threonine 72-19-5 11.910
L-Tryptophan 73-22-3 3.060
L-Tyrosine disodium salt dihydrate 69847-15-0 3.920
L-Valine 72-18-4 35.130
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 13.960
D-Biotin 58-85-5 0.015
D-Ca-Pantothenate 137-08-6 0.238
Folic acid 59-30-3 0.790
Niacinamide 98-92-0 0.037
Pyridoxine hydrochloride 58-56-0 0.061
Riboflavin 83-88-5 0.038
Thiamine hydrochloride 67-03-8 0.337
Vitamin B12 68-19-9 0.407
myo-Inositol 87-89-8 18.020
OTHERS
Adenine hydrochloride 2922-28-1 30.880
D-Glucose 50-99-7 1081.000
HEPES 7365-45-9 6600.000
Phenol red sodium salt 34487-61-1 1.242
Putrescine dihydrochloride 333-93-7 0.161
Sodium pyruvate 113-24-6 55.000
Thioctic acid 1077-28-7 0.206
Thymidine 50-89-5 0.727
Custom formulation: Growth factor co-formulations, adjusted trace element concentrations, custom glucose, and pH modifications available. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system, with particular attention to trace element dissolution and particulate removal during MCDB formulation.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016–certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm — reduces ionic and organic-carbon background in trace-element MCDB formulations where contaminants can compete with zinc, copper, and iron uptake.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch production, per-lot traceability, Certificate of Analysis for every batch — trace element concentrations verified against specification.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.

Endotoxin — USP <85> BET

LAL assay; release specification < 0.05 EU/mL, tested per manufacturing batch.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 290–330 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every batch on request.

Certificate of Analysis: Request for any DCP-M151H-B1X batch at support@diagnocine.com.
Product Comparison

How DCP-M151H-B1X compares

FluxMPS™ DCP-M151H-B1X vs. conventional 0.22 µm–filtered MCDB 151 −Bicarbonate +HEPES formulations.

Parameter DCP-M151H-B1X (FluxMPS™) Conventional MCDB 151 −Bicarbonate +HEPES
(0.22 µm filtered)		 Standard Alt. MCDB
(0.22 µm filtered)
Grade Microfluidics Suitable Not specified Not specified
MCDB 151 without sodium bicarbonate + HEPES — CO2-independent keratinocyte/CHO culture check_circle Yes cancel No cancel No
Trace element delivery Particle-free (0.04 µm) May contain aggregates May contain aggregates
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 µm) cancel No cancel No
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes (Method 1) cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Not specified Not specified
Manufacturing QMS ISO 13485:2016 Not specified Not specified
Microfluidic channel compatibility check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-M151H-B1X MCDB 151 −Bicarbonate +HEPES.

Yes. DCP-M151H-B1X is processed through a quadruple-stage filtration system reaching a 0.04 µm final pore size, delivering low particulate levels for MPS, OoC, ToC, and LoC platforms. The trace element profile is delivered particle-free, reducing the risk of trace metal aggregate deposition in chip channels, and the endotoxin release specification is < 0.05 EU/mL.
FluxMPS™ uses four sequential filtration passes — 0.1 µm prefiltration I, 0.04 µm final filtration I, 0.1 µm prefiltration II, and 0.04 µm final filtration II — reaching a 0.04 µm final pore size, five times finer than the 0.22 µm membranes used for conventional filtration. For MCDB media containing trace elements, this reduces the risk of trace metal salt micro-aggregates that pass through 0.22 µm filters and could otherwise deposit in microfluidic channels.
Sodium bicarbonate is removed so that 28 mM HEPES alone provides all pH buffering without CO2. HEPES (pKa 7.3 at 37°C) maintains stable pH near 7.4 during open-top chip incubation, without the pH drift associated with bicarbonate/CO2 systems. This formulation still contains glucose, L-glutamine, and sodium pyruvate as carbon and nitrogen sources; no additional carbon source substitution is required before use. Contact support@diagnocine.com for cell-type-specific customization.
No. This formulation is CO2-independent — 28 mM HEPES maintains pH without gas supplementation, making it suitable for open-top chip and atmospheric culture platforms.
Yes. MCDB media are designed for low-protein or serum-free conditions but can be supplemented with growth factors (EGF, bFGF, VEGF, etc.), dialyzed FBS protein (FBSP, 0.5–2%), or antibiotics. Any serum or protein-containing supplement should be filtered at 0.2 µm through a low-protein-binding PES or PVDF membrane before addition; do not use a 0.04 µm membrane for supplement filtration, as it will strip serum proteins and lipoproteins. Contact support@diagnocine.com for custom co-formulations.
DCP-M151H-B1X is produced to meet a release specification of < 0.05 EU/mL by LAL assay (USP <85>). Endotoxin is controlled per manufacturing batch: every batch is tested before release and must meet this specification before it ships. A Certificate of Analysis reporting the batch result is available on request.
Yes. A full CoA per batch covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), and particulate count (USP <788> Method 1), along with lot number, expiry, and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting MCDB 151 −Bicarbonate +HEPES for Human Epidermal Keratinocytes culture and organ-on-a-chip applications.

  1. Ham RG, McKeehan WL. Media and growth requirements. Methods Enzymol. 1979;58:44–93. doi:10.1016/S0076-6879(79)58127-6
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Boyce ST, Ham RG. Calcium-regulated differentiation of normal human epidermal keratinocytes in chemically defined clonal culture and serum-free serial culture. J Invest Dermatol. 1983;81(1 Suppl):33s–40s. doi:10.1111/1523-1747.ep12540422
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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