FluxMPS™ MCDB 131 Medium w/o Sodium Bicarbonate: 1X Liquid
FluxMPS™ MCDB 131 Medium w/o Sodium Bicarbonate: 1X Liquid
FluxMPS™ DCP-M131-B1X is a Microfluidics Suitable, ultra-filtered MCDB 131 medium formulated without sodium bicarbonate, engineered for serum-free or low-protein culture of Human Microvascular Endothelial Cells (HMVEC) and related vascular cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off, it delivers a release specification of < 0.05 EU/mL endotoxin and a trace-element package designed to reach the culture dissolved rather than as particulate aggregate. Formulation: [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] Trace Elements | [-] Sodium Bicarbonate.
- Formulated for Human Microvascular Endothelial Cells (HMVEC), HUVEC and HAEC in CO2-independent, low-protein or serum-free culture systems
- Trace-element package — zinc, copper, selenium, manganese, molybdenum, nickel and vanadium salts — supporting metalloenzyme and antioxidant function without serum
- Quadruple-stage filtration train: 0.1 µm Prefiltration I → 0.04 µm Final filtration I → 0.1 µm Prefiltration II → 0.04 µm Final filtration II, reaching a 0.04 µm final cut-off
- Endotoxin release specification < 0.05 EU/mL by LAL assay (USP <85>), controlled per manufacturing batch
- Sodium bicarbonate omitted for CO2-independent buffering; HEPES (15–25 mM) recommended for atmospheric incubation
- Low glucose (1000 mg/L) formulation with L-glutamine (1461 mg/L) and sodium pyruvate (110 mg/L)
- Manufactured under an ISO 13485:2016 quality management system with a per-lot Certificate of Analysis
- Custom pH, glucose, trace-element concentration and growth-factor co-formulations available on request
- Cell typesHuman Microvascular Endothelial Cells (HMVEC)
- Glucose1000 mg/L (1.0 g/L, Low Glucose)
- Trace ElementsPresent — Zn, Cu, Se, Mn, Mo, Ni, V
- AppearanceRed-pink colored, clear solution (phenol red present)
- pH (USP <791>)7.4
- Osmolality (USP <785>)235 – 275 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1 µm ×2 + 0.04 µm ×2 (Quadruple-stage)
- Storage2–8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
Available pack sizes: 500 mL, 1000 mL.
Engineered where standard media fails
MCDB media formulated with trace-element salts can develop subvisible particulate aggregates when filtered at 0.22 µm under typical manufacturing conditions. These aggregates clog microfluidic channels, disrupt trace-metal bioavailability, and introduce variable endotoxin loads into sensitive primary cell models. FluxMPS™ addresses these failure modes with four-stage sub-0.04 µm filtration and a < 0.05 EU/mL endotoxin release specification.
Particle-free trace-element delivery
0.04 µm final filtration is intended to keep trace-metal salts in solution — reducing the risk of aggregate deposition in chip channels or culture vessels where precipitation would alter bioavailability.
Serum-free primary cell support
The trace-element composition supports metalloenzyme activity (e.g. superoxide dismutase, glutathione peroxidase, carbonic anhydrase) relevant to primary cell survival and function without serum supplementation.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm) is used to minimize trace-metal and organic-carbon contaminants that could compete with intended trace-element uptake in low-serum primary cell culture.
Low endotoxin release specification
< 0.05 EU/mL by LAL assay (USP <85>), tested per manufacturing batch, to minimize the risk of endotoxin-driven inflammatory signaling (e.g. via TLR4) confounding primary-cell phenotype in serum-free conditions.
Rich nutrient profile
The MCDB 131 trace-element and amino-acid profile is designed to support clonal growth and serum-free survival of endothelial and related primary cell types.
Customization on demand
pH, glucose, trace-element concentrations, HEPES and growth-factor co-formulations available. Contact support@diagnocine.com.
Quadruple-stage filtration system
Four serial filtration stages reaching a final 0.04 µm polish — a repeated prefilter-plus-final-filter pair, run twice — delivering ultra-low particulate levels and particle-free trace-element delivery beyond what conventional 0.22 µm filtered MCDB media provide.
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1
0.1 µm Prefiltration I
Removes large aggregates, including trace-metal salt precipitates, and provides the first mycoplasma-retentive pass (mycoplasma organisms are typically 0.2–0.3 µm); protects the first 0.04 µm cartridge and downstream chip geometries from fouling.
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2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and trace-metal micro-aggregates that pass a standard 0.22 µm filter.
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3
0.1 µm Prefiltration II
A second, dedicated prefilter protecting the second 0.04 µm cartridge; second-pass mycoplasma-retentive filtration redundancy.
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4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill and finish in an ISO Class 5 (Class 100) laminar-flow workstation.
Performance vs. conventional MCDB media
FluxMPS™ DCP-M131-B1X is processed to a 0.04 µm final cut-off across four filtration passes, compared to a single 0.22 µm pass typical of conventional MCDB 131 formulations.
© Diagnocine® — DCP-M131-B1X
Specialized cell models & OoC applications
FluxMPS™ DCP-M131-B1X was formulated for Human Microvascular Endothelial Cells (HMVEC), HUVEC and HAEC in CO2-independent, low-protein or serum-free conditions. The 0.04 µm filtered trace-element matrix is intended for use in microfluidic chip architectures where conventional MCDB media may cause trace-metal particulate fouling.
Automated Bioreactors & Robotics
This product is Microfluidics Suitable at a 0.04 µm final cut-off. A separate, optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available for automated bioreactor perfusion where sub-0.04 µm trace-metal colloids may contribute to valve fouling.
- Extended nanoparticulate reduction: the 0.01 µm MPS Grade variant is filtered beyond this product's 0.04 µm cut-off
- Valve & sensor protection: intended to reduce micro-fouling from trace-element aggregates in automated systems
- Extended perfusion stability: supports consistent trace-element delivery over multi-week primary cell culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Organ-on-a-Chip & MPS
0.04 µm final filtration is intended to reduce the risk of microchannel clogging, with trace elements delivered particle-free.
Vascular Biology
FluxMPS™ MCDB 131 (w/o sodium bicarbonate) is filtered to reduce sub-mycoplasma-range particulates and deliver trace elements for vascular biology models cultured in serum-free or low-protein conditions.
Endothelium-on-Chip
Formulated to support endothelium-on-chip models requiring controlled trace-element delivery and low particulate load in serum-free or low-protein conditions.
CO2-Independent Vascular Culture
Bicarbonate-free formulation intended for CO2-independent culture; add HEPES for atmospheric incubation across chip compartments with variable gas control.
Open-Top Vascular Chips
Suited to open-top vascular chip formats and point-of-care endothelial assays where CO2 control cannot be uniformly maintained.
Antioxidant & Redox Metabolism
Trace-element package (Zn, Cu, Se, Mn) supports metalloenzyme-dependent antioxidant systems (e.g. SOD, glutathione peroxidase) relevant to oxidative-stress research.
Analytical release specifications
Every lot is released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] Low Glucose (1000 mg/L), [+] L-Glutamine, [+] Sodium Pyruvate, [+] Phenol Red, [+] Calcium, [+] Magnesium, [+] Trace Elements | [-] Sodium Bicarbonate (None added) |
| Appearance | Red-pink colored, clear solution (phenol red present) |
| Glucose | 1000 mg/L (1.0 g/L, Low Glucose) |
| Trace Elements | Present — Zn, Cu, Se, Mn, Mo, Ni, V salts |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 235 – 275 mOsm/kg H2O |
| Total ingredients | 52 components across 4 categories |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL (batch release specification; see § Manufacturing & Compliance) |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) laminar flow |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, protect from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO2 requirement | CO2-independent formulation (no sodium bicarbonate buffer); HEPES (15–25 mM) recommended for atmospheric incubation |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Grade | Microfluidics Suitable (0.04 µm final cut-off) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
MCDB 131 (without sodium bicarbonate): 52 ingredients verified per lot with CAS numbers for full raw-material traceability, including a trace-element package for metalloenzyme support and antioxidant activity.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Ammonium metavanadate | 7803-55-6 | 0.0006 |
| Ammonium molybdate tetrahydrate | 12054-85-2 | 0.0037 |
| Calcium chloride dihydrate | 10035-04-8 | 235.200 |
| Cupric sulfate pentahydrate | 7758-99-8 | 0.0012 |
| Disodium hydrogen phosphate anhydrous | 7558-79-4 | 71.000 |
| Ferrous sulfate heptahydrate | 7782-63-0 | 0.278 |
| Magnesium sulfate anhydrous | 7487-88-9 | 1204.000 |
| Manganese sulfate | 7785-87-7 | 0.00015 |
| Molybdic acid tetrahydrate (ammonium) | 12054-85-2 | 0.0037 |
| Nickel chloride hexahydrate | 7791-20-0 | 0.000071 |
| Potassium chloride | 7447-40-7 | 298.200 |
| Sodium chloride | 7647-14-5 | 6428.400 |
| Sodium metasilicate nonahydrate | 13517-24-3 | 2.842 |
| Sodium selenite | 10102-18-8 | 0.0052 |
| Zinc sulfate heptahydrate | 7446-20-0 | 0.0003 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 2.250 |
| L-Alanine | 56-41-7 | 2.670 |
| L-Arginine hydrochloride | 1119-34-2 | 63.210 |
| L-Asparagine monohydrate | 5794-13-8 | 15.010 |
| L-Aspartic acid | 56-84-8 | 13.310 |
| L-Cysteine hydrochloride monohydrate | 7048-04-6 | 35.120 |
| L-Glutamic acid | 56-86-0 | 44.130 |
| L-Glutamine | 56-85-9 | 1461.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 41.920 |
| L-Isoleucine | 73-32-5 | 65.600 |
| L-Leucine | 61-90-5 | 131.200 |
| L-Lysine hydrochloride | 657-27-2 | 182.600 |
| L-Methionine | 63-68-3 | 14.920 |
| L-Phenylalanine | 63-91-2 | 33.040 |
| L-Proline | 147-85-3 | 11.510 |
| L-Serine | 56-45-1 | 31.530 |
| L-Threonine | 72-19-5 | 11.910 |
| L-Tryptophan | 73-22-3 | 4.080 |
| L-Tyrosine disodium salt dihydrate | 69847-15-0 | 22.520 |
| L-Valine | 72-18-4 | 117.100 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 13.960 |
| D-Biotin | 58-85-5 | 0.0073 |
| D-Ca-Pantothenate | 137-08-6 | 11.915 |
| Folinic acid (Calcium) | 1492-18-8 | 0.5115 |
| Niacinamide | 98-92-0 | 6.105 |
| Pyridoxine hydrochloride | 58-56-0 | 2.056 |
| Riboflavin | 83-88-5 | 0.0038 |
| Thiamine hydrochloride | 67-03-8 | 3.373 |
| Vitamin B12 | 68-19-9 | 0.0136 |
| myo-Inositol | 87-89-8 | 7.208 |
| OTHERS | ||
| Adenine hydrochloride | 2922-28-3 | 1.720 |
| D-Glucose | 50-99-7 | 1000.000 |
| Phenol red sodium salt | 34487-61-1 | 12.421 |
| Putrescine dihydrochloride | 333-93-7 | 0.0002 |
| Sodium pyruvate | 113-24-6 | 110.000 |
| Thioctic acid | 1077-28-7 | 0.0021 |
| Thymidine | 50-89-5 | 0.0242 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a multi-layer quality system, with particular attention to trace-element dissolution and particulate removal during MCDB formulation.
ISO 13485:2016 Quality Management
Manufactured under an ISO 13485:2016-certified quality system. Final QC at the Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm feed water, used for MCDB trace-element formulations where ionic contaminants could compete with zinc, copper and selenium uptake.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch production with per-lot traceability and a Certificate of Analysis for every lot; trace-element concentrations verified against specification.
Endotoxin — USP <85> BET
LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL.
Particulate — USP <788> Method 1
Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Target: 235 – 275 mOsm/kg H2O.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-M131-B1X compares
FluxMPS™ DCP-M131-B1X vs. conventional 0.22 µm-filtered MCDB 131 (without sodium bicarbonate) formulations.
| Parameter | DCP-M131-B1X (FluxMPS™) | Conventional MCDB 131 (0.22 µm filtered) | Standard Alt. MCDB (0.22 µm filtered) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not specified | Not specified |
| MCDB 131 without sodium bicarbonate — CO2-independent endothelial culture with trace elements | check_circle Yes | cancel No | cancel No |
| Trace element delivery | Particle-free intent (0.04 µm) | May contain aggregates | May contain aggregates |
| Final filtration pore size | 0.04 µm | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle Yes (0.1 µm) | cancel No | cancel No |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> Method 1 particulate tested | check_circle Yes | cancel No | cancel No |
| Water quality | Type 1, 18.2 MΩ·cm | Purified water | Purified water |
| Manufacturing QMS | ISO 13485:2016 | ISO 9001 or none | ISO 9001 or none |
| Microfluidic channel compatibility | check_circle Microfluidics Suitable | cancel Risk of clogging | cancel Risk of clogging |
| Custom formulation | check_circle Available | cancel Fixed | cancel Fixed |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-M131-B1X MCDB 131 (without sodium bicarbonate).
Supporting literature
Key publications supporting MCDB 131 (without sodium bicarbonate) for Human Microvascular Endothelial Cell (HMVEC) culture and organ-on-a-chip applications.
- Ham RG, McKeehan WL. Media and growth requirements. Methods Enzymol. 1979;58:44–93. doi:10.1016/S0076-6879(79)58127-6
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Campisi M, et al. 3D self-organized microvascular model of the human blood-brain barrier. Biomaterials. 2018;180:117–129. doi:10.1016/j.biomaterials.2018.07.014
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
