FluxMPS™ Leibovitz's L-15 Medium: 1X Liquid
FluxMPS™ Leibovitz's L-15 Medium: 1X Liquid
Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid Leibovitz's L-15 medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). This CO2-independent, bicarbonate-free formulation uses galactose in place of glucose and is manufactured under an ISO 13485:2016 quality management system. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.
- Quadruple-stage filtration (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size for microchannel-safe delivery
- Galactose-based carbohydrate source (0.9 g/L) replaces glucose, avoiding lactic acid buildup during CO2-independent culture
- Bicarbonate-free, CO2-independent formulation — no CO2 incubator required
- L-Glutamine (300 mg/L) and Sodium Pyruvate (550 mg/L) included as ready energy and nitrogen substrates
- Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85> BET), tested per manufacturing batch
- Manufactured under an ISO 13485:2016 quality management system; final QC and packaging at Diagnocine, Totowa, NJ
- Phenol red (11 mg/L) included as a visual pH indicator
- Custom pH, HEPES, salts & nutrient composition available on request — support@diagnocine.com
- Galactose900 mg/L ([+])
- L-Glutamine300 mg/L ([+])
- Sodium Pyruvate550 mg/L ([+])
- pH (USP <791>)7.4
- Osmolality (USP <785>)300 - 340 mOsm/kg
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1µm ×2 + 0.04µm ×2
- Storage2-8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
- ShippingCold pack (2–8°C)
Engineered where standard media fails
Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels and complicating long-duration perfusion. FluxMPS™ closes that gap.
Microchannel-safe purity
0.04 µm final filter and USP <788> Method 1 (light obscuration) particulate control support unobstructed flow in narrow microfluidic channels.
Total metabolic control
A galactose-based carbon source with defined amino acid levels supports controlled metabolic studies, including Warburg-effect and glycolysis-inhibition research.
Ultrapure-grade water
Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) for low trace-metal and organic-carbon background throughout manufacturing.
Low background for imaging
Ultra-low particulate baseline supports confocal live-cell imaging, fluorescent biosensors, and automated high-content analysis on chip.
Rich, stable nutrient profile
4× BME amino acid and vitamin concentrations with micro-batch precision for tight lot-to-lot consistency during long-duration MPS perfusion.
Customization on demand
pH, glucose, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
A validated four-stage sequential filtration train reaching a final pore size of 0.04 µm — capturing particulates and organisms that pass through conventional 0.22 µm filtration.
-
1
0.1 µm Prefiltration I
Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm final filter cartridge.
-
2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter, including organisms in the 0.2–0.3 µm mycoplasma size range.
-
3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill & finish.
Performance vs. conventional media
By USP <788> Method 1 (light obscuration), FluxMPS™ media are formulated for approximately 5× fewer particles ≥10 µm compared with standard 0.22 µm-filtered media — supporting long-duration perfusion without channel occlusion.
© Diagnocine® — DCP-LL151X
Optimized for next-generation cell biology platforms
FluxMPS™ Leibovitz's L-15 Medium: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and CO2-independent culture are critical.
Automated Bioreactors & Robotics
For fully automated bioreactor and robotic handling systems, Diagnocine offers an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation — a separate line from the 0.04 µm Microfluidics Suitable product on this page.
- Total particulate exclusion for closed-loop automated feed systems
- Valve & sensor protection in long-duration robotic perfusion
- Extended perfusion stability for unattended culture runs
Inquiry Required: The 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com.
Micro Physiological System (MPS) & Chip
Ultra-low particulate media for perfusion in organ chips, tissue chips (ToC), and body-on-a-chip (BoC) devices.
Warburg Effect & Metabolic Research
Galactose-based, glucose-free formulation supports Warburg-effect studies and aerobic glycolysis research.
iPSC-Derived Models
Ultra-clean, low-endotoxin baseline minimizes non-specific signals in iPSC differentiation and functional organoid readouts.
Endothelial & Primary Cells
Particle-free, CO2-independent perfusion media for TEER measurement, endothelial monolayer integrity, and primary cell culture.
Metabolic Flux Analysis
Chemically defined formulation for ¹³C metabolic tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Ultra-low particulate baseline for confocal microscopy, fluorescent biosensors, and automated imaging on chip.
Full technical specification
Every lot of FluxMPS™ Leibovitz's L-15 Medium: 1X Liquid is released against comprehensive multi-parameter QC specifications.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, Phenol Red, Calcium, Magnesium, Sodium Pyruvate (galactose-based, glucose-free) |
| Appearance | Clear, pink-red solution (phenol red pH indicator present) |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 300 - 340 mOsm/kg H₂O |
| Glucose | Not added / None (galactose serves as the carbon source) |
| L-Glutamine | 300 mg/L ([+]) |
| Sodium Pyruvate | 550 mg/L ([+]) |
| Phenol Red | 11 mg/L |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> | < 0.05 EU/mL (batch release — see Manufacturing & Compliance) |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm <788> M1 | Compliant (light obscuration) |
| Particulate ≥25 µm <788> M1 | Compliant |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing | ISO 13485:2016 ISO |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage | 2-8°C, protect from light |
| Freeze–thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping | Cold pack (2–8°C) |
| CO₂ requirement | Not required (CO₂-independent, bicarbonate-free formulation) |
| Parameter | Specification |
|---|---|
| Raw material grade | Pharmaceutical/research grade CoA |
| Manufacturing QMS | ISO 13485:2016 ISO |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch; Totowa, NJ, USA |
| Intended use | RUO only |
Available pack sizes: 500 mL, 1000 mL.
Full composition (mg/L)
Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 35 components across 4 category groups (Inorganic Salts, Amino Acids, Vitamins, Others), organized into 3 tabs below. All values are per-lot verified and reported on the Certificate of Analysis (CoA).
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 185.000 |
| Magnesium chloride hexahydrate | 7791-18-6 | 200.000 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Potassium phosphate monobasic | 7778-77-0 | 60.000 |
| Sodium chloride | 7647-14-5 | 8000.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 190.120 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| L-Alanine | 56-41-7 | 225.000 |
| Glycine | 56-40-6 | 200.000 |
| L-Arginine (free base) | 74-79-3 | 500.000 |
| L-Asparagine | 70-47-3 | 250.000 |
| L-Cysteine (free base) | 52-90-4 | 120.000 |
| L-Glutamine | 56-85-9 | 300.000 |
| L-Histidine (free base) | 71-00-1 | 250.000 |
| L-Isoleucine | 73-32-5 | 250.000 |
| L-Leucine | 61-90-5 | 125.000 |
| L-Lysine hydrochloride | 657-27-2 | 94.000 |
| L-Methionine | 63-68-3 | 75.000 |
| L-Phenylalanine | 63-91-2 | 125.000 |
| L-Serine | 56-45-1 | 200.000 |
| L-Threonine | 72-19-5 | 300.000 |
| L-Tryptophan | 73-22-3 | 20.000 |
| L-Tyrosine disodium salt | 276.160 | |
| L-Valine | 72-18-4 | 100.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 1.000 |
| D-Ca-Pantothenate | 137-08-6 | 1.000 |
| Folic acid | 59-30-3 | 1.000 |
| Nicotinamide | 98-92-0 | 1.000 |
| Pyridoxine hydrochloride | 58-56-0 | 1.000 |
| Riboflavin-5-phosphate sodium salt | 130-40-5 | 0.100 |
| Thiamine hydrochloride | 67-03-8 | 1.000 |
| i-Inositol | 87-89-8 | 2.000 |
| OTHERS | ||
| D-Galactose | 59-23-4 | 900.000 |
| Phenol red sodium salt | 34487-61-1 | 11.000 |
| Sodium pyruvate | 113-24-6 | 550.000 |
Manufacturing & compliance
Every batch is subjected to multi-parameter lot-release testing before distribution.
ISO 13485:2016 QMS
Manufactured by ISO 13485-certified suppliers. Final packaging, QA and testing at Diagnocine R&D Center; customization at Diagnocine Precision, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm feed water with low trace-metal and organic-carbon content, used throughout manufacturing.
ISO Class 5 Fill & Finish
Validated ISO Class 5 laminar-flow workstation with real-time particle monitoring, preserving filtration gains in the final container.
Micro-Batch Precision
Small-batch production for tight lot-to-lot consistency, supporting reproducible long-duration MPS experiments.
Endotoxin — USP <85> BET
LAL assay (Bacterial Endotoxins Test); assay sensitivity 0.005 EU/mL. Release specification: < 0.05 EU/mL, tested per manufacturing batch.
Particulate — USP <788> Method 1
Light-obscuration particle count; formulated for approximately 5× fewer particles ≥10 µm vs. 0.22 µm-filtered media.
Osmolality — USP <785>
Freezing-point depression osmometry. Release range: 300 - 340 mOsm/kg H₂O.
Certificate of Analysis (CoA)
Full CoA per lot. Request at support@diagnocine.com with lot number.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-LL151X compares
Critical differences in grade, filtration, mycoplasma barrier, water quality, and QC depth versus conventional 0.22 µm filtered L-15 media.
| Parameter | DCP-LL151X (FluxMPS™) | Standard L-15 (0.22 µm) | Competitor L-15 (0.22 µm) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not specified | Not specified |
| Formulation definition | [+] L-Glutamine, Phenol Red, Calcium, Magnesium, Sodium Pyruvate (galactose-based, glucose-free) | Standard | Standard |
| Final filtration pore size | 0.04 µm (40 nm) | 0.22 µm | 0.22 µm |
| Number of filtration stages | 4 (Quadruple) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle | cancel | cancel |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> particulate compliance | check_circle ~5× lower | cancel | cancel |
| Water quality | Ultrapure Type 1, 18.2 MΩ·cm | Unspecified | Unspecified |
| Manufacturing QMS | ISO 13485:2016 | Varies | Varies |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation | check_circle On request | cancel | cancel |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ Leibovitz's L-15 Medium: 1X Liquid (DCP-LL151X).
Supporting literature
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
- Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
- van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
- Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
- Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
- Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x