Hektoen Enteric Agar Plates/W Ampicillin Sodium Salt
Catalog.No: DCP-HEAPAS
Features:
Uses: Isolation and differentiation of Salmonella and Shigella from food, water, and environmental samples in food safety and public health labs, and fecal specimens in clinical diagnostics
pH: 7.5 ± 0.2
Size: 100 mm
Concentration: 100 µg/mL
Storage: Keep away from light at 4°C
Shipping Condition: Blue ice
Apeearance: green with a yellowish cast; clear to slightly opalescent gel.
Sterile
Shelf Life: 2 Weeks
*All agar plates are manufactured with ultrapure water, deionized and demineralized to minimize ionic and organic contaminants. The premium ISO certified agar solution is sterilized by a full cycle of autoclaving. Each 100mm plate has 20mL of agar medium in premium plastic dish.
Description:
Hektoen Enteric (HE) Agar with ampicillin sodium salt is a modified, moderately selective and differential plate medium that combines the standard HE formulation with β-lactam selection to suppress ampicillin-susceptible bacteria while recovering and differentiating ampicillin-resistant enteric targets such as Salmonella or Shigella. HE contains bile salts that are able to inhibit most Gram-positive organisms and many non-pathogenic Gram-negative enterics. Carbohydrates support acid production by non-target fermenters which form yellow to orange/salmon colonies. Peptone utilization by Salmonella and Shigella yields alkaline reactions keeping colonies and surrounding medium green to-blue green, and sodium thiosulfate as well as ferric ammonium citrate detect H2S production. H2S-positive colonies develop black centers or become black.
Ampicillin is a β-lactam that inhibits cell wall synthesis in susceptible bacteria, particularly many Enterobacterales and Gram-positive organisms lacking effective β-lactamases. HE and ampicillin when combined, suppresses ampicillin-susceptible background flora, enriching for ampicillin-resistant Salmonella, Shigella, or other engineered/clinical enteric strains. Functionally, this reduces background growth and potential swarming by susceptible organisms, improving isolation and visualization of resistant target colonies on the HE background.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
