Hektoen Enteric Agar Plates/W Carbenicillin
Catalog.No: DCP-HEAPC
Features:
Uses: Isolation and differentiation of Salmonella and Shigella from food, water, and environmental samples in food safety and public health labs, and fecal specimens in clinical diagnostics
pH: 7.5 ± 0.2
Size: 100 mm
Concentration: 50 µg/mL
Storage: Keep away from light at 4°C
Shipping Condition: Blue ice
Apeearance: green with a yellowish cast; clear to slightly opalescent gel.
Sterile
Shelf Life: 4 weeks
*All agar plates are manufactured with ultrapure water, deionized and demineralized to minimize ionic and organic contaminants. The premium ISO certified agar solution is sterilized by a full cycle of autoclaving. Each 100mm plate has 20mL of agar medium in premium plastic dish.
Description:
Hektoen Enteric (HE) agar with carbenicillin is a modified moderately selective and differential medium in which HE agar is supplemented with a β-lactam antibiotic to suppress carbenicillin-susceptible bacteria while preserving HE’s ability to differentiate Salmonella and Shigella from other enteric flora. The composition of HE includes bile salts which inhibits most Gram-positive bacteria or fully inhibit many non-pathogenic coliforms and other commensal Gram-negative rods. The carbohydrates and pH indicators distinguish fermenters from non-fermenters. Fermenting non-target enterics produce acid changing the colonies and surrounding medium yellow to orange/salmon. Salmonella and shigella act as non-fermenters and rely on peptone mainly, producing alkaline end products that maintain colonies and the surrounding colony medium green to blue-green. Sodium thiosulfate and ferric ammonium citrate detect H2S by forming black ferrous sulfide and as a result, many Salmonella produce green/blue colonies with black centers.
Carbenicillin is a broad-spectrum β-lactam that inhibits cell wall synthesis, particularly in many Gram-negative rods and some Gram-positives that lack β-lactamases. The combination of HE and carbenicillin suppresses carbenicillin-susceptible background flora (Gram-negative and Gram-positive) while allowing carbenicillin resistant enteric targets to grow. This enhances selectivity beyond bile salts alone, reducing background growth and making resistant Salmonella/Shigella or engineered enterics easier to isolate and read on the HE differential background.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
