Hektoen Enteric Agar Plates/W Penicillin G
Catalog.No: DCP-HEAPPG
Features:
Uses: Isolation and differentiation of Salmonella and Shigella from food, water, and environmental samples in food safety and public health labs, and fecal specimens in clinical diagnostics
pH: 7.5 ± 0.2
Size: 100 mm
Concentration: 25 µg/mL
Storage: Keep away from light at 4°C
Shipping Condition: Blue ice
Apeearance: green with a yellowish cast; clear to slightly opalescent gel.
Sterile
Shelf Life: 2 weeks
*All agar plates are manufactured with ultrapure water, deionized and demineralized to minimize ionic and organic contaminants. The premium ISO certified agar solution is sterilized by a full cycle of autoclaving. Each 100mm plate has 20mL of agar medium in premium plastic dish.
Description:
Hektoen Enteric (HE) agar with Penicillin G is a modified, selective and differential plate medium in which the standard HE formulation is combined with a β-lactam antibiotic to suppress penicillin-susceptible Gram-positive flora (and some Gram-negatives) while recovering and differentiating enteric bacteria, especially Salmonella and Shigella. HE contains bile salts that inhibit most Gram-positive organisms and suppress many non-pathogenic Gram-negative enterics. Carbohydrates support acid production by fermenting non-target flora, which form yellow to orange/salmon colonies with associated color changes in the surrounding medium. Salmonella and Shigella behave as non-fermenters of these plate carbohydrates under HE conditions, relying mainly on peptone; they produce alkaline reactions that keep colonies and surrounding medium green to blue-green. Sodium thiosulfate and ferric ammonium citrate detect H2S production and many colonies that are H2S positive develop black centers or become black due to ferrous sulfide deposition.
Penicillin G is a β-lactam antibiotic that inhibits cell wall synthesis by binding penicillin-binding proteins, particularly in many Gram-positive organisms and some Gram-negative rods that lack effective β-lactamases. The combination of HE and Penicillin G results in further suppression of penicillin-susceptible Gram-positive flora and some Gram-negative rods, enhancing the selectivity of HE by limiting growth of organisms that might otherwise survive bile salts and interfere with isolation or visualization of resistant target enteric bacteria.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
