FluxMPS™ Ham's F-12 Nutrient Mixture w/o Sodium Bicarbonate, Phenol Red, Folic Acid: 1X Liquid

FluxMPS™ Ham's F-12 Nutrient Mixture w/o Sodium Bicarbonate, Phenol Red, Folic Acid: 1X Liquid

FluxMPS™ DCP-H12-BRF1X is a Microfluidics Suitable, ultra-filtered Ham's F-12 formulation engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic tissue models. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulated without sodium bicarbonate, phenol red, or folic acid for CO₂-independent, imaging-clean, DHFR-selection culture protocols.

• Formulated without sodium bicarbonate, phenol red, or folic acid — enabling CO₂-independent culture, autofluorescence-free imaging, and DHFR-mediated selection pressure.
• Quadruple-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) reaching a 0.04 µm final cut-off for microfluidic channel compatibility.
• Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch.
• 1801.60 mg/L D-glucose, 146 mg/L L-glutamine, and 110.1 mg/L sodium pyruvate provide a defined carbon and nitrogen source.
• Manufactured under an ISO 13485:2016 quality management system with aseptic ISO Class 5 fill and finish; final QA at Diagnocine, Totowa, NJ.
• 44 ingredients verified per lot with CAS traceability, including zinc, putrescine, hypoxanthine, and thymidine specific to the Ham's F-12 formulation.
• Custom pH, salt, and nutrient adjustments available on request — contact support@diagnocine.com.

Engineered where standard media fails

Conventional 0.22 µm–filtered media passes mycoplasma-sized particulates, subvisible aggregates, and endotoxin fragments that clog microfluidic channels and interfere with sensor readouts. FluxMPS™ addresses these failure modes with a validated four-stage sub-0.04 µm filtration train.

Quadruple-stage filtration system

Four serial filtration stages, arranged as two dedicated prefilter-plus-final-filter pairs, reach a 0.04 µm final polish under aseptic fill conditions — supporting sub-mycoplasma-scale purity for microfluidic applications.

1. 1

   0.1 µm Prefiltration I

   Removes large particulates and protein aggregates; protects the first 0.04 µm cartridge.

2. 2

   0.04 µm Final filtration I

   First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.

3. 3

   0.1 µm Prefiltration II

   Second dedicated prefilter, protecting the second 0.04 µm cartridge.

4. 4

   0.04 µm Final filtration II — Polish

   Ultimate polishing filter; aseptic fill and finish.

Designed for next-generation cell models

FluxMPS™ DCP-H12-BRF1X supports platforms from single-channel microfluidic chips to multi-organ body-on-a-chip systems, as well as classical DHFR-selection and monolayer culture.

Automated Bioreactors & Robotics

An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation is available on request for automated bioreactor perfusion and robotic liquid handlers.

• Total Particulate Exclusion: 0.01 µm filtration removes nanoparticulate aggregates
• Valve & Sensor Protection: Reduces micro-fouling risk on solenoid valves and inline optical sensors
• Extended Perfusion Stability: Supports consistent nutrient delivery over weeks-long culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Full composition (mg/L)

44 ingredients verified per lot with CAS numbers for full raw-material traceability. Ham's F-12 contains zinc, putrescine, hypoxanthine, and thymidine in addition to standard amino acids and vitamins.

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

How DCP-H12-BRF1X compares

FluxMPS™ DCP-H12-BRF1X vs. conventional 0.22 µm–filtered Ham's F-12 formulations.

Comparison figures from published supplier specifications, accessed September 2, 2026. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

Frequently asked questions

Common questions about FluxMPS™ DCP-H12-BRF1X.

Supporting literature

Key peer-reviewed publications supporting ultra-filtered, Microfluidics Suitable media in organ-on-a-chip and microfluidic research, and the original Ham's F-12 formulation.

1. Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53:288–293. doi:10.1073/pnas.53.2.288
2. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
3. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
4. Kaufman RJ. Selection and coamplification of heterologous genes in mammalian cells. Methods Enzymol. 1990;185:537–566. doi:10.1016/0076-6879(90)85044-o
5. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
8. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j