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FluxMPS™ Ham's F-12 Nutrient Mixture w/o L-Glutamine: 2X Liquid

Product#: DCP-H12-Q2X
$96.25
DCP-H12-Q2X
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FluxMPS™ Ham's F-12 Nutrient Mixture w/o L-Glutamine: 2X Liquid

Double-strength (2X) Ham's F-12 — 3.6 g/L glucose in the concentrate, 1.8 g/L at 1X — for CHO and clonal culture, epithelial cells, chondrocytes, serum-reduced medium development and semisolid culture, ultra-filtered through a quadruple-stage 0.1 µm / 0.04 µm filtration system. With a sodium bicarbonate buffer, it is approximately 5× cleaner than conventional 0.22 µm-filtered media by particulate count.

  • 2X concentrate: mix with an equal volume of a compatible solution or matrix for a final 1X medium
  • Ham's F-12 nutrients including zinc, putrescine, hypoxanthine, thymidine, sodium pyruvate, linoleic acid and lipoic acid
  • Quadruple-stage filtration: 0.1 µm membrane filtered twice, 0.04 µm membrane filtered twice
  • 0.04 µm nano-filtration final polish acts as a mycoplasma barrier
  • Endotoxin < 0.05 EU/mL (USP <85> BET)
  • Formulation: [+] 3.6 g/L Glucose, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] Phenol Red; [−] L-Glutamine, [−] HEPES
  • Ultrapure Type 1 water (18.2 MΩ·cm); ISO Class 5 (Class 100) aseptic fill in Totowa, NJ
  • Custom formulations available — pH, glucose, HEPES, salts, and nutrient composition on request
DCP-H12-Q2X|UNSPSC 41116155Cell culture media
Catalog No. DCP-H12-Q2X · Size: 500 mL
Ham's F-12 Nutrient Mixture w/o L-Glutamine: 2X Liquid
  • D-Glucose3,603 mg/L
  • L-GlutamineNot included
  • Sodium Pyruvate220 mg/L
  • BufferNaHCO₃ 2,352 mg/L
  • Phenol Red2.48 mg/L
  • AppearanceRed
  • pH7.4 (± 0.04)
  • Endotoxin< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Format2X liquid, 500 mL
ISO 13485:2016USP <85> <785> <788>RUO
Why FluxMPS™

Engineered where standard media fails

Conventional Ham's F-12 is sterile-filtered once at 0.22 µm. That pore size can pass mycoplasma and leaves subvisible particulates that settle in microchannels, foul membranes and hydrogels, and add noise to optical readouts.[11,12] FluxMPS™ Ham's F-12 keeps Ham's formulation and removes that failure mode.

filter_alt

Microchannel-safe purity

A 0.04 µm final filter and USP <788> particulate control keep subvisible debris out of chips, tubing, membranes and matrices.

target

Total metabolic control

Defined 3,603 mg/L D-glucose, no L-glutamine and 220 mg/L sodium pyruvate in the 2X (half that at 1X).

water_drop

Ultrapure-grade water

Made with Ultrapure Type 1 water (18.2 MΩ·cm) and released at < 0.05 EU/mL endotoxin by USP <85>.

visibility

Low background for imaging

Particle-depleted medium reduces scatter and debris artifacts in colony counting, confocal imaging and on-chip sensing.

science

Matrix-ready 2X strength

46 components at double strength, so agar, hydrogels, supplements or treatment solutions can be added 1:1 without diluting the medium below 1X.

tune

Customization on demand

pH, glucose, HEPES, salts, and nutrient composition on request — contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Every lot of DCP-H12-Q2X is membrane filtered at 0.1 µm twice and 0.04 µm twice — a ready-to-dilute 2X Ham's F-12 polished to a 0.04 µm final pore size, a purity level single-pass 0.22 µm media cannot reach.

  1. 01

    0.1 µmPre-filtration I

    Removes large particulates and aggregates and extends the life of the downstream filters.

  2. 02

    0.04 µmPre-filtration II

    Retains fine particulates, bacteria and mycoplasma.

  3. 03

    0.1 µmSterile-filtration I

    Second-pass redundancy that catches anything shed or bypassed upstream.

  4. 04

    0.04 µmSterile-filtration II — Final Polish

    Final nano-polish immediately before ISO Class 5 aseptic fill.

Performance vs. conventional media

By particulate count, FluxMPS™ Ham's F-12 is approximately 5× cleaner than conventional 0.22 µm-filtered media, lowering clog risk in narrow channels and long perfusion runs.

5×
cleaner by particulate count vs. 0.22 µm media
0.04
µm final filtration pore size
Sterility & mycoplasma assurance: no bacterial or fungal growth is observed after 14 days of incubation, as per USP specification (USP <71>), and the 0.04 µm stages provide USP <63>-equivalent mycoplasma assurance.[11]
FluxMPS™ Ham's F-12 Nutrient Mixture w/o L-Glutamine: 2X Liquid (DCP-H12-Q2X) Quadruple-stage filtration system diagram: 0.1 μm pre-filtration, 0.04 μm pre-filtration, 0.1 μm sterile filtration and 0.04 μm final polish for MPS-grade Ham's F-12 cell culture media used in CHO, clonal, organ-on-a-chip and microfluidic applications, Diagnocine
Figure 1. Quadruple-stage filtration: 0.1 µm pre-filtration, 0.04 µm pre-filtration, 0.1 µm sterile filtration and 0.04 µm final polish before ISO Class 5 fill.
© Diagnocine® — DCP-H12-Q2X
Applications

Where FluxMPS™ Ham's F-12 fits

Ham’s F-12 Nutrient Mixture is a nutrient-rich basal medium containing amino acids, vitamins, inorganic salts, glucose, trace elements, and other components for in vitro mammalian-cell research. Its formulation includes zinc, putrescine, hypoxanthine, thymidine, sodium pyruvate, and lipid-related constituents. Originally developed for serum-free single-cell plating and clonal growth of Chinese hamster ovary (CHO) cells, it is also used with selected epithelial cells, chondrocytes, and other laboratory cell systems when appropriately supplemented.[1,3]

The medium provides a basal nutritional foundation for cell-growth, colony-formation, nutrient-response, and research-medium development studies. It contains no added proteins or growth factors, so serum or defined supplements should be selected according to the cell type and validated protocol; its historical serum-free CHO application does not establish universal serum-free suitability.[5]

Ham’s F-12 Nutrient Mixture, 2X, is a double-strength version of the corresponding 1X basal formulation, intended for research preparations that require substantial dilution. When mixed with an equal volume of a compatible solution that contributes none of the corresponding basal ingredients, it is designed to provide those ingredients at their intended final 1X concentrations. The concentrate may be evaluated for preparation of semisolid or hydrogel-containing research cultures where an aqueous matrix component would otherwise dilute the basal medium.[4] The purpose is dilution compensation, not routine culture at twice the usual nutrient and salt concentrations.

It is useful for:

  • CHO growth and clonal-culture research
  • Epithelial-cell research
  • Chondrocyte research
  • Selected primary-cell research
  • In vitro clonal-toxicity assays
  • Serum-reduced or serum-free medium development
  • Semisolid and matrix-culture development

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated bioreactors, liquid-handling robots and closed perfusion loops with fine valves and in-line sensors, an optional 10 nm (0.01 µm) ultra-filtered grade of this medium is available.

  • Total Particulate Exclusion: 0.01 µm filtration removes virtually all particulates down to the nanometer scale.
  • Valve & Sensor Protection: keeps micro-valves, pumps and optical or electrochemical sensors free of deposits.
  • Extended Perfusion Stability: supports long, uninterrupted perfusion runs with fewer line changes.

Inquiry Required: the 0.01 µm grade is made on request — email support@diagnocine.com with your catalog number and volume.

Cell Lines

CHO Growth & Clonal Culture

Developed for serum-free single-cell plating and clonal growth of Chinese hamster ovary cells; a reference basal medium for CHO research.[1,3]

CHOSingle-cell platingClonal growth
Cell Biology

Epithelial Cells & Chondrocytes

Used with selected epithelial cells, chondrocytes and other primary cells when appropriately supplemented after dilution to 1X.[1]

EpithelialChondrocytesPrimary cells
Toxicology

Clonal-Toxicity Assays

Colony-formation readouts in a clonal-growth medium support in vitro clonal-toxicity and nutrient-response studies.[1]

Colony formationClonal toxicityNutrient response
Media Development

Serum-Reduced & Semisolid Media

A double-strength base for serum-reduced or serum-free medium development and for semisolid or hydrogel cultures.[4,5]

Serum-freeHydrogelsSoft agar
Microfluidics

Organ-on-a-Chip & MPS

Microchannel-safe medium for perfused organ-, tissue- and body-on-a-chip platforms.[8,9,10]

OoCToCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Fewer particles means cleaner fields for colony counting, confocal imaging and on-chip biosensors.

ConfocalColony countingBiosensors
Technical Specifications

Specifications & quality control

Release specifications for DCP-H12-Q2X. Lot-specific values are reported on the Certificate of Analysis.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] 3.6 g/L Glucose, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [+] Phenol Red; [−] L-Glutamine, [−] HEPES
Concentration 2X (dilute 1:1 to 1X)
Appearance Red
pH 7.4 (± 0.04) USP <791>
Osmolality Measured per lot; reported on CoA USP <785>
D-Glucose 3,603 mg/L (3.6 g/L; 1.8 g/L at 1X)
L-Glutamine Not included
Sodium Pyruvate 220 mg/L
Sodium Bicarbonate 2,352 mg/L
Phenol Red 2.48 mg/L
HEPES Not included
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin < 0.05 EU/mL USP <85> BET
Sterility No bacterial or fungal growth after 14 days of incubation USP <71>
Mycoplasma 0.04 µm barrier filtration USP <63> equiv.
Particulate ≥10 µm Meets limits USP <788> M2
Particulate ≥25 µm Meets limits USP <788> M2
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016 ISO
Fill environment ISO Class 5 (Class 100) ISO
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8 °C, protected from light
Freeze-thaw Do not freeze
Shelf life 12 months; use before the expiry date on the product label
Shipping condition Ice Pack
CO₂ requirement CO₂-controlled incubator after dilution to 1X; sodium bicarbonate 2,352 mg/L in the 2X, 1,176 mg/L at 1X (classically equilibrated with 10% CO₂; many protocols use 5%)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade High-purity, cell-culture-tested components
Traceability Lot-traceable; CoA per lot
Manufacturing QMS ISO 13485:2016 ISO
Regulatory alignment 21 CFR Part 820 (cGMP) aligned
Production method Micro-batch 2X liquid, quadruple-stage filtered; aseptic fill and finish in Totowa, NJ
Intended use For Research Use Only (RUO)
Note: pH and sodium bicarbonate concentration of the prepared medium are critical factors affecting cell growth. This is also influenced by amount of medium and volume of culture vessel used (surface to volume ratio). For example, in large bottles, such as Roux bottles, pH tends to rise perceptibly as a significant volume of carbon dioxide is released. Therefore, optimal conditions of pH, sodium bicarbonate concentration and surface to volume ratio must be determined for each cell type. We recommend stringent monitoring of pH. If needed, pH can be adjusted by using sterile 1N HCl or 1N NaOH or by bubbling in carbon dioxide. If required, supplements can be added to the medium prior to or after filter sterilization observing sterility precautions.
Formulation

Full composition (mg/L)

DCP-H12-Q2X is 2X Ham's F-12 with 3.6 g/L glucose, sodium pyruvate, sodium bicarbonate and Phenol Red. It does not contain L-glutamine nor HEPES. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines. All 46 components are listed below at 2X strength; values are per-lot release basis and are halved after 1:1 dilution.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 88.200
Copper sulphate pentahydrate 7758-99-8 0.0050
Ferrous sulphate heptahydrate 7782-63-0 1.668
Magnesium chloride anhydrous 7786-30-3 115.300
Potassium chloride 7447-40-7 447.200
Sodium bicarbonate 144-55-8 2352.000
Sodium chloride 7647-14-5 15198.000
Sodium phosphate dibasic anhydrous 7558-79-4 284.080
Zinc sulphate heptahydrate 7446-20-0 1.726
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 15.000
L-Alanine 56-41-7 17.820
L-Arginine hydrochloride 1119-34-2 421.400
L-Asparagine monohydrate 5794-13-8 30.020
L-Aspartic acid 56-84-8 26.600
L-Cysteine hydrochloride monohydrate 7048-04-6 70.240
L-Glutamic acid 56-86-0 29.400
L-Histidine hydrochloride monohydrate 5934-29-2 41.920
L-Isoleucine 73-32-5 7.880
L-Leucine 61-90-5 26.200
L-Lysine hydrochloride 657-27-2 73.000
L-Methionine 63-68-3 8.960
L-Phenylalanine 63-91-2 9.920
L-Proline 147-85-3 69.000
L-Serine 56-45-1 21.000
L-Threonine 72-19-5 23.800
L-Tryptophan 73-22-3 4.080
L-Tyrosine disodium salt dihydrate 122666-87-4 15.620
L-Valine 72-18-4 23.400
Component CAS Number mg/L
VITAMINS
Biotin 58-85-5 0.0146
Choline chloride 67-48-1 27.920
D-Ca-Pantothenate 137-08-6 0.960
Folic acid 59-30-3 2.640
Nicotinamide 98-92-0 0.074
Pyridoxine hydrochloride 58-56-0 0.124
Riboflavin 83-88-5 0.076
Thiamine hydrochloride 67-03-8 0.680
Vitamin B12 68-19-9 2.720
i-Inositol 87-89-8 36.000
OTHERS
D-Glucose 50-99-7 3603.200
Hypoxanthine sodium salt 45738-97-4 9.540
Linoleic acid 60-33-3 0.168
Lipoic acid 1077-28-7 0.420
Phenol Red 34487-61-1 2.480
Putrescine dihydrochloride 333-93-7 0.322
Sodium pyruvate 113-24-6 220.200
Thymidine 50-89-5 1.460
Customization: Custom formulations available — pH, glucose, HEPES, salts, and nutrient composition on request. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Manufactured under an ISO 13485:2016 quality management system; aseptic fill and finish in Totowa, NJ, with every lot released against compendial test methods.

verified

ISO 13485:2016 QMS

Documented, audited quality management system aligned with 21 CFR Part 820 (cGMP).

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm water as the formulation base for low ionic and endotoxin background.

biotech

ISO Class 5 Fill & Finish

Aseptic filling in an ISO Class 5 (Class 100) environment in Totowa, NJ, right after the final 0.04 µm polish.

assignment

Micro-Batch Precision

Small, tightly controlled batches for consistent composition and pH from lot to lot.

Endotoxin — USP <85> BET

Released at < 0.05 EU/mL.

Particulate — USP <788> Method 2

Subvisible particles ≥10 µm and ≥25 µm within compendial limits.

Osmolality — USP <785>

Measured per lot and reported on the CoA.

Documentation / CoA

Appearance, pH 7.4 (± 0.04), 14-day sterility, endotoxin and osmolality per lot.

A lot-specific Certificate of Analysis is available for every lot — request it at support@diagnocine.com.
Product Comparison

How DCP-H12-Q2X compares

Same Ham's F-12 chemistry, different purity class. Conventional columns describe typical single-pass 0.22 µm-filtered catalog media.

Parameter DCP-H12-Q2X (FluxMPS™) Conventional Ham's F-12, 1X (0.22 µm filtered) Conventional 2X Ham's F-12 (0.22 µm filtered)
Formulation 2X Ham's F-12; free of L-Glutamine 1X F-12, ready to use Double-strength, for semisolid culture
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 1 1
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin specification < 0.05 EU/mL Often not specified at this level Often not specified at this level
USP <788> particulate compliance check_circle cancel cancel
Ultrapure Type 1 water (18.2 MΩ·cm) check_circle Varies Varies
ISO 13485:2016 manufacturing check_circle Varies Varies
Microfluidic channel compatibility check_circle cancel cancel
Custom formulation check_circle cancel cancel
FAQ

Frequently asked questions

Common questions about using DCP-H12-Q2X for semisolid, 3D, microfluidic and conventional culture.

Yes. DCP-H12-Q2X is quadruple-stage filtered to a 0.04 µm final pore size, so it carries far fewer subvisible particles than 0.22 µm media — approximately 5× cleaner by particulate count. That lowers the risk of clogging microchannels, valves and membranes in organ-on-a-chip, hydrogel-based 3D and microfluidic perfusion systems.[8,9,10]
The medium is filtered four times: 0.1 µm twice and 0.04 µm twice. A single 0.22 µm pass can let mycoplasma and fine particulates through; the 0.04 µm stages act as a mycoplasma barrier and polish out sub-micron particles before ISO Class 5 aseptic fill.[11]
L-glutamine breaks down in liquid medium over time, releasing ammonia that can stress sensitive cultures; a glutamine-free base lets you add fresh L-glutamine or a stable dipeptide just before use. Our complete 2X formulation carries 292 mg/L L-glutamine (146 mg/L at 1X). Users are advised to review the literature for supplementation and physiological growth requirements specific to each cell type.[1,2]
Yes, once diluted to 1X. The medium is bicarbonate-buffered (1,176 mg/L at 1X), designed for a CO₂ atmosphere (classically 10%, commonly 5%); keep time in room air short.
Mix 2X Ham's F-12 with an equal volume of a compatible solution that contributes none of the basal ingredients — an aqueous agar, agarose or hydrogel component, supplements or treatment solutions — so glucose (1.8 g/L), amino acids, vitamins and salts end up at their 1X concentration.[4] The purpose is dilution compensation, not routine culture at twice the usual nutrient and salt concentrations. Serum, antibiotics and other supplements can be added before or after filter sterilization, observing sterility precautions.
The endotoxin specification is < 0.05 EU/mL, tested by the USP <85> bacterial endotoxins test (BET) and reported on the lot Certificate of Analysis. The medium is made with Ultrapure Type 1 water (18.2 MΩ·cm).
Yes. Each lot ships with a CoA covering appearance (red), pH 7.4 (± 0.04), sterility (no bacterial or fungal growth after 14 days of incubation per USP), endotoxin and osmolality results. Request copies at support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed background on Ham's F-12 and CHO culture, serum-free and soft-agar culture, Phenol Red, organ-on-a-chip culture and mycoplasma control.

  1. Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53(2):288–293. doi:10.1073/pnas.53.2.288
  2. Eagle H. Nutrition needs of mammalian cells in tissue culture. Science. 1955;122(3168):501–504. doi:10.1126/science.122.3168.501
  3. Tjio JH, Puck TT. Genetics of somatic mammalian cells. II. Chromosomal constitution of cells in tissue culture. J Exp Med. 1958;108(2):259–268. doi:10.1084/jem.108.2.259
  4. Borowicz S, Van Scoyk M, Avasarala S, et al. The soft agar colony formation assay. J Vis Exp. 2014;(92):e51998. doi:10.3791/51998
  5. Barnes D, Sato G. Methods for growth of cultured cells in serum-free medium. Anal Biochem. 1980;102(2):255–270. doi:10.1016/0003-2697(80)90151-7
  6. Morton HJ. A survey of commercially available tissue culture media. In Vitro. 1970;6(2):89–108. doi:10.1007/BF02616111
  7. Berthois Y, Katzenellenbogen JA, Katzenellenbogen BS. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc Natl Acad Sci USA. 1986;83(8):2496–2500. doi:10.1073/pnas.83.8.2496
  8. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760–772. doi:10.1038/nbt.2989
  9. Low LA, Mummery C, Berridge BR, Austin CP, Tagle DA. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20(5):345–361. doi:10.1038/s41573-020-0079-3
  10. Leung CM, de Haan P, Ronaldson-Bouchard K, et al. A guide to the organ-on-a-chip. Nat Rev Methods Primers. 2022;2:33. doi:10.1038/s43586-022-00118-6
  11. Drexler HG, Uphoff CC. Mycoplasma contamination of cell cultures: incidence, sources, effects, detection, elimination, prevention. Cytotechnology. 2002;39(2):75–90. doi:10.1023/A:1022913015916
  12. Halldorsson S, Lucumi E, Gómez-Sjöberg R, Fleming RMT. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens Bioelectron. 2015;63:218–231. doi:10.1016/j.bios.2014.07.029

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