FluxMPS™ Ham's F-12 Nutrient Mixture w/o Sodium Bicarbonate, Folic Acid: 1X Liquid
FluxMPS™ Ham's F-12 Nutrient Mixture w/o Sodium Bicarbonate, Folic Acid: 1X Liquid
FluxMPS™ DCP-H12-BF1X is a Microfluidics Suitable, ultra-filtered Ham's F-12 Nutrient Mixture engineered for microphysiological systems (MPS), organ-on-a-chip (OoC), and microfluidic tissue models. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] 1801.60 mg/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Phenol Red | [-] Sodium Bicarbonate, [-] Folic Acid.
- Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final pore size for microfluidic channel-safe purity below 100 µm
- Formulated without sodium bicarbonate — CO₂-independent; HEPES or another organic buffer required for pH control
- Formulated without folic acid — supports DHFR-based selection protocols
- Endotoxin specification: < 0.05 EU/mL (LAL, USP <85>), released per manufacturing batch
- Contains 1801.60 mg/L D-glucose, 146.00 mg/L L-glutamine, and 110.10 mg/L sodium pyruvate as primary carbon/energy sources
- Contains phenol red (1.24 mg/L) as a pH indicator — a phenol-red-free variant is available for fluorescence-sensitive assays
- Manufactured under an ISO 13485:2016 quality management system; final QC performed at Diagnocine, Totowa, NJ
- Custom pH, salts, and nutrient adjustments available on request
- Formulation[+] 1801.60 mg/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Phenol Red | [-] Sodium Bicarbonate, [-] Folic Acid
- AppearanceOrange-colored, clear solution
- pH (USP <791>)7.4
- Osmolality (USP <785>)260–300 mOsm/kg H2O
- Endotoxin (USP <85>)< 0.05 EU/mL
- Sterility (USP <71>)No growth / 14 days
- Filtration0.1 µm ×2 + 0.04 µm ×2 (4 stages)
- Total ingredients45
- Storage2–8°C, away from light
- Shelf Life12 months from date of manufacture, unopened
Engineered where standard media fails
Conventional 0.22 µm–filtered media passes mycoplasma-sized organisms, subvisible particulates, and cellular debris that accumulate in microfluidic channels and corrupt sensor signals. FluxMPS™ addresses these failure modes with a four-stage 0.04 µm filtration train.
Microchannel-safe purity
0.04 µm final filtration and USP <788> Method 1 particulate compliance support safe perfusion across a wide range of microfluidic chip geometries.
Total metabolic control
Selective exclusion of sodium bicarbonate and folic acid supports CO₂-independent culture and DHFR-based selection, while glucose, glutamine, and pyruvate remain available as carbon and energy sources.
Ultrapure-grade water
Ultrapure Type 1 water (18.2 MΩ·cm) controls trace-metal and organic-carbon background during manufacture.
Low background for imaging
Ultra-low particulate baseline supports confocal microscopy and biosensor platforms. This formulation contains phenol red; request our phenol-red-free variant for assays sensitive to background fluorescence.
Rich, stable nutrient profile
45 ingredients verified per lot; micro-batch production with full traceability.
Customization on demand
pH, glucose, salts, HEPES, and nutrients adjustable per your protocol. Contact support@diagnocine.com.
Quadruple-stage filtration system
-
1
0.1 µm Prefiltration I
Removes large particulate, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.
-
2
0.04 µm Final filtration I
First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass through a 0.22 µm filter.
-
3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm cartridge.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; ISO Class 5 aseptic fill & finish.
Performance vs. conventional media
© Diagnocine® — DCP-H12-BF1X
Designed for next-generation cell models
FluxMPS™ DCP-H12-BF1X supports demanding platforms from single-channel microfluidic chips to multi-organ body-on-a-chip systems.
Automated Bioreactors & Robotics
An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available on request for automated bioreactor perfusion and robotic liquid handlers.
- Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
- Valve & Sensor Protection: Reduces micro-fouling of solenoid valves and inline optical sensors
- Extended Perfusion Stability: Supports consistent nutrient delivery over weeks-long culture
Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.
Micro Physiological System (MPS) & Chip
0.04 µm–filtered media reduces the risk of microchannel clogging in complex multi-organ chip architectures.
DHFR-Selection & Mammalian Cell Culture
Folic-acid-free, CO₂-independent formulation supports DHFR-based selection systems and HEPES-buffered mammalian cell culture.
iPSC-Derived Models
Ultra-low endotoxin (< 0.05 EU/mL release specification) supports sensitive iPSC protocols.
Endothelial & Primary Cells
Low-particulate, endotoxin-controlled media for HUVEC monolayer integrity monitoring.
Metabolic Flux Analysis
Defined, bicarbonate-free formulation provides a clean background for ¹³C isotope tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol-red-free medium; this formulation contains phenol red.
Microscopy & Optical Sensing
Ultra-low particulate baseline for confocal and biosensor platforms; phenol-red-free variant available on request for autofluorescence-sensitive assays.
Analytical release specifications
Every lot released against the full specification matrix. CoA: support@diagnocine.com.
| Parameter | Specification |
|---|---|
| Formulation | [+] 1801.60 mg/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Phenol Red | [-] Sodium Bicarbonate, [-] Folic Acid |
| Appearance | Orange-colored, clear solution |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 260–300 mOsm/kg H2O |
| Glucose | 1801.60 mg/L |
| L-Glutamine | 146.00 mg/L |
| Sodium Pyruvate | 110.10 mg/L |
| Phenol Red | 1.240 mg/L |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> BET | < 0.05 EU/mL |
| Sterility USP <71> | No growth / 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate ≥10 µm USP <788> Method 1 | NMT 25/mL |
| Particulate ≥25 µm USP <788> Method 1 | NMT 3/mL |
| Water purity | Type 1, 18.2 MΩ·cm |
| Manufacturing std. | ISO 13485:2016 |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2–8°C, away from light |
| Freeze-thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack |
| CO₂ requirement | CO₂-independent; use HEPES or organic buffer for pH control (bicarbonate-free formulation) |
| Parameter | Specification |
|---|---|
| Raw material grade | Reagent / cell culture grade |
| Traceability | Full lot traceability per ISO 13485 |
| Manufacturing QMS ISO | ISO 13485:2016 certified |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch, per-lot QC release |
| Intended use | Research Use Only (RUO) |
Full composition (mg/L)
45 ingredients verified per lot with CAS numbers for full raw-material traceability. Ham's F-12 contains zinc, putrescine, hypoxanthine, and thymidine in addition to standard amino acids and vitamins.
| Component | CAS Number | mg/L |
|---|---|---|
| INORGANIC SALTS | ||
| Calcium chloride dihydrate | 10035-04-8 | 44.100 |
| Copper sulfate pentahydrate | 7758-99-8 | 0.0025 |
| Ferrous sulfate heptahydrate | 7782-63-0 | 0.834 |
| Magnesium chloride anhydrous | 7786-30-3 | 57.650 |
| Potassium chloride | 7447-40-7 | 223.600 |
| Sodium chloride | 7647-14-5 | 7599.000 |
| Sodium phosphate dibasic anhydrous | 7558-79-4 | 142.040 |
| Zinc sulfate heptahydrate | 7446-20-0 | 0.863 |
| Component | CAS Number | mg/L |
|---|---|---|
| AMINO ACIDS | ||
| Glycine | 56-40-6 | 7.500 |
| L-Alanine | 56-41-7 | 8.910 |
| L-Arginine hydrochloride | 1119-34-2 | 210.700 |
| L-Asparagine monohydrate | 5794-13-8 | 15.010 |
| L-Aspartic acid | 56-84-8 | 13.300 |
| L-Cystine dihydrochloride | 30925-07-6 | 35.120 |
| L-Glutamic acid | 56-86-0 | 14.700 |
| L-Glutamine | 56-85-9 | 146.00 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 20.960 |
| L-Isoleucine | 73-32-5 | 3.940 |
| L-Leucine | 61-90-5 | 13.100 |
| L-Lysine hydrochloride | 657-27-2 | 36.500 |
| L-Methionine | 63-68-3 | 4.480 |
| L-Phenylalanine | 63-91-2 | 4.960 |
| L-Proline | 147-85-3 | 34.500 |
| L-Serine | 56-45-1 | 10.500 |
| L-Threonine | 72-19-5 | 11.900 |
| L-Tryptophan | 73-22-3 | 2.040 |
| L-Tyrosine disodium salt dihydrate | 69847-15-0 | 7.810 |
| L-Valine | 72-18-4 | 11.700 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Biotin | 58-85-5 | 0.0073 |
| Choline chloride | 67-48-1 | 13.960 |
| D-Ca-Pantothenate | 137-08-6 | 0.480 |
| Nicotinamide | 98-92-0 | 0.037 |
| Pyridoxal hydrochloride | 65-22-5 | 0.062 |
| Riboflavin | 83-88-5 | 0.038 |
| Thiamine hydrochloride | 67-03-8 | 0.340 |
| Vitamin B12 | 68-19-9 | 1.360 |
| OTHERS | ||
| i-Inositol | 87-89-8 | 18.000 |
| D-Glucose | 50-99-7 | 1801.600 |
| Hypoxanthine Sodium Salt | 45738-97-4 | 4.770 |
| Linoleic acid | 60-33-3 | 0.084 |
| Lipoic acid | 1077-28-7 | 0.210 |
| Phenol red Sodium Salt | 34487-61-1 | 1.240 |
| Putrescine dihydrochloride | 333-93-7 | 0.161 |
| Sodium pyruvate | 113-24-6 | 110.100 |
| Thymidine | 50-89-5 | 0.730 |
Manufacturing & compliance
Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system spanning raw materials, in-process controls, and final-product testing.
ISO 13485:2016 Quality Management
Manufactured under ISO 13485:2016-certified facilities. Final QA at Diagnocine R&D Center, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm resistivity — controls trace-metal and organic-carbon (TOC) background during manufacture.
ISO Class 5 Fill & Finish
Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.
Micro-Batch Precision
Small-batch production, full per-lot traceability, Certificate of Analysis for every lot.
Endotoxin — USP <85> BET
LAL assay, assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL per batch.
Particulate — USP <788> Method 1
Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).
Osmolality — USP <785>
Freezing-point osmometry. Target: 260–300 mOsm/kg H2O.
Documentation & CoA
Full CoA with raw-material traceability available for every lot on request.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-H12-BF1X compares
FluxMPS™ DCP-H12-BF1X vs. published specifications from other cell culture media suppliers.
| Parameter | DCP-H12-BF1X (FluxMPS™) | Comparison (published supplier specifications) |
|---|---|---|
| Grade | Microfluidics Suitable | Not specified |
| Bicarbonate-free, folic-acid-free, CO₂-independent DHFR selection base | check_circle Yes | Not specified |
| Final filtration pore size | 0.04 µm | Not specified |
| Number of filtration stages | 4 (Quadruple-stage) | Not specified |
| Mycoplasma barrier filtration | check_circle 0.1 µm mycoplasma-retentive | Not specified |
| Endotoxin (release specification) | FluxMPS™ — < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
| USP <788> Method 1 particulate tested | check_circle Yes | Not specified |
| Water quality | Type 1, 18.2 MΩ·cm | Not specified |
| Manufacturing QMS | ISO 13485:2016 | Not specified |
| Microfluidic channel compatibility | check_circle Yes | Not specified |
| Custom formulation | check_circle Available | Not specified |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ DCP-H12-BF1X.
Supporting literature
Key peer-reviewed publications supporting Microfluidics Suitable, ultra-filtered media in organ-on-a-chip and microfluidic research.
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Ham RG. Clonal growth of mammalian cells in a chemically defined, synthetic medium. Proc Natl Acad Sci USA. 1965;53:288–293. doi:10.1073/pnas.53.2.288
- Urlaub G, Chasin LA. Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity. Proc Natl Acad Sci USA. 1980;77:4216–4220. doi:10.1073/pnas.77.7.4216
- Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
- Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
- Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
- Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j
