Fungal gDNA Kit (4 Preps)
Cat. No.: BMG-GD2416-00
Size: 4 Preps
Description:
A Fungal gDNA Kit is a specialized tool designed for the extraction and purification of genomic DNA from fungal samples.
Applications:
- These kits are essential for isolating genomic DNA to study fungal genetics, gene function, and molecular mechanisms.
- Extracted DNA can be used to detect and identify fungal pathogens in clinical or environmental samples using PCR or sequencing methods.
- The kits enable studies on fungal genetics and gene function.
- They allow detection of fungi in environmental or food samples.
- Some rapid extraction methods are suitable for PCR screening of fungal mutants.
- Certain kits can extract fungal DNA from infected plant material for studying plant-pathogen interactions.
- Some methods are optimized for extracting DNA from fungal spores collected in traps.
- The kits enable extraction of fungal DNA from complex environmental or clinical samples for microbiome sequencing.
- Extracted DNA can be analyzed to detect antifungal resistance genes.
- These kits allow detection of fastidious or unculturable fungi directly from clinical specimens.
- Lysis buffers to break open fungal cell walls.
- Enzymes for protein digestion.
- Binding buffers to facilitate DNA adsorption.
- Wash buffers to remove impurities.
- Elution buffer to recover purified DNA.
- Spin columns or magnetic beads for DNA purification.
- Specialized lysis techniques (enzymatic digestion, bead-beating).
- Spin column-based purification with silica membrane technology.
- Rapid extraction (typically <1 hour) from tough-to-lyse fungi.
- Suitable for fresh and dried samples, including polysaccharide-rich tissues.
- Processing capacity of 20-200 mg wet tissue or 30-50 mg dry tissue.
- Effective removal of PCR inhibitors.
- Typical yield of 5-30 μg purified DNA.
- Elution volumes of 50-100 μL.
- Suitable for various downstream applications (PCR, sequencing).
- Options for manual or automated processing.
- Available in different formats for various throughput needs.
- All-inclusive kits with necessary reagents and buffers.
Kumar M, Mugunthan M. Evaluation of three DNA extraction methods from fungal cultures. Med J Armed Forces India. 2018 Oct;74(4):333-336. doi: 10.1016/j.mjafi.2017.07.009. Epub 2017 Oct 5. PMID: 30449918; PMCID: PMC6224647.
Gudisa R, Harchand R, Rudramurthy SM. Nucleic-Acid-Based Molecular Fungal Diagnostics: A Way to a Better Future. Diagnostics (Basel). 2024 Feb 29;14(5):520. doi: 10.3390/diagnostics14050520. PMID: 38472992; PMCID: PMC10931343.
Gudisa R, Harchand R, Rudramurthy SM. Nucleic-Acid-Based Molecular Fungal Diagnostics: A Way to a Better Future. Diagnostics (Basel). 2024 Feb 29;14(5):520. doi: 10.3390/diagnostics14050520. PMID: 38472992; PMCID: PMC10931343.
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