iPGell

Product#: PG20-1
$500.00
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iPGell

Cat. No. PG20-1
Size 1 Kit
Storage -20 oC

Description

Making a section of floating cells or 3D cultured cells (such as embryoid body, spheroid, etc) is burdensome work for researchers.
Conventional methods are cytospin, smear slide, or agarose gel but these method gives damages to your cell sample as the cells are dried or heated during sample preparaiton.
Moreover, for cytospin or smear slide method, you need to start again from sample preparation.

iPGell will greatly help you to achieve this just by using two components.
Cells can be rapidly jellified with unheated condition. Any toxic components affect to cells are not contained.
Jellified sample can be soaked into fixation solution and then you can make a sample block (frozen / paraffin) like the way to make tissue block.

Features

  • Rapid and Easy procedure
    - Just mixing solution A and B, ~2 minutes protocol.

  • Lower damage to Cells (RNA and Protein)
    - as sample is not heated.

  • Various analysis can be done from same sample
    - frozen/paraffin blocks enable you to make a section when you need.
Procedure

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Samples
 
  • Primary cultured cell
  • Embryoid body
  • Spheroid
  • iPS cells / ES cells
  • Specific cell population sorted by cell sorter

Experiment Examples
 
  • Morphorogy of mouse derived embryoid body by HE staining

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Embryoid body A (x100)

Embryoid body A (x400)

Embryoid body B (x100)

Embryoid body C (x100)

 
  • Expression analysis of 3D cultured (spheroid culture) cell
    PG20-1_6.png 
    IHC (Fixed frozen section, x 1000)
    Antibody D (Brown) / Counter Stain (Blue)

  • Expression analysis of cultured cell by in situ hybridization

    Gene (Blue) / Counter Stain (Pink)

    Gene (Blue) / Counter Stain (Pink)

    Gene (Blue) / Counter Stain (Pink)

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    Gene E (x400)

    Gene F (x400)

    Negative Control (x400)


For more information please see the video :


References
 
 

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