HBsAg L-protein(gtD)(Recombinant)

Cat. No.: FNK-BCL-AGD-01

Size: 100μg

Storage: -20℃ (3 years)


Introduction:

HBsAg (also called Bionanocapsule) is the surface antigen of Hepatitis B virus (HBV). HBsAg is known to be present in blood if HBV infected patients, and used as a important diagnostic maker of HBV infection. HBsAg forms a small lipid particle（diameter 50 to 60nm）in which many antigen protein are present. Each antigen protein has three domains, called S, Pre-S1 and Pre-S2. L-type antigen contains all the three domains, M-type antigen contains S- and Pre-S2, and S-type antigen consisted of only S-domains. During the process of HBV infection, Pre-S1 domain plays a key role for host (hepatic) cell recognition, and Pre-S2 domain is essential for cell penetration. In infected patients, hepatic cells produces a lot of non-infectious S-type HBsAg (HBV mimetics without genome). The detection of serum Pre-S2 and Pre-S1 antigen is, therefore, very important to reveal the mechanism of HBV infection. Beacle has over a decade of R&D history on HBsAg and related materials, and developed the production process of various HBsAgs.




Hepatitis B virus (HBV) expresses three types of surface antigens, i.e. S-, M-, and L-protein. L-protein is composed of S-, Pre-S2, and Pre-S1 region. The deletion of Pre-S1 region forms M-protein and further deletion of Pre-S2 region results in S-protein. Most of commercially available HBsAg is composed of either S-protein alone or a mixture of Sand M-proteins. The Pre-S1 region is known to be the hepatic cell recognition site and to be important in the HBV infection. Pre-S1 region is known to have variation of amino acid sequence depending genotype.

Specifications:

Source : Saccharomyces cerevisiae

Appearance : Lyophilized white powder

Structure: Particle (L-protein inserted in lipid bilayer) with mean particle size of about 70nm by dynamic light scattering method (about 20 nm by electronic microscope)

Dissolve : For 100 µg vial, added 500 µL of water to the vial that makes a antigen solution at 0.2 mg/mL in PBS (137 mM NaCl, 8.1 mM Na₂HPO4 * 12H2O, 2.68 mM KCl, 1.47 mM KH₂PO₄, pH 7.2 - 7.4) containing 1% sucrose. 

Purity : over 95%  

Usage : HBV mimetics, standard antigen for ELISA, western, and others. Tagged Pre-S1 peptides are better for ELISA plate immobilization.

*Caution :L protein may be adsorbed on plastic tubes especially low concentration (below 100ng/mL). Please use protein low bind tubes at lower concentration.


SDS-PAGE


HBsAg, L-protein of genotype A to D were subjected for SDS-PAGE (Silver staining) at 100ng


Amino acid sequence:
KVRQGMGQNLSTSNPLGFFPDHQLDPAFRANTANPDWDFNPNKDTWPDANKVGAGAFGLGFTPPHGGLLGWSPQAQGILQTLPANPPPASTNRQTGRQPTPLSPPLRNTHPQAMQWNSTTFHQTLQDPRVRGLYFPAGGSSSGAVNPVPTTASPLSGDPALNMENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGGTTVCLGQNSQSPTSNHSPTSCPPTCPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQGMLPVCPLIPGSSTTSTGPCRTCTTPAQGTSMYPSCCCTKPSDGNCTCIPIPSSWAFGKFLWEWASARFSWLSLLVPFVQWFVGLSPTVWLSVIWMMWYWGPSLYSILSPFLPLLPIFFCLWVYI