FluxMPS™ RPMI 1640 Medium w/o Phenol Red: 1X Liquid

verified ISO 13485 Certified Manufacturing

FluxMPS™ RPMI 1640

FluxMPS™ DCP-RPMI-R1X is an MPS-grade, ultra-filtered RPMI 1640 formulation engineered for Hematopoietic cells and related cell models on organ-on-a-chip (OoC) and microphysiological system (MPS) platforms. Processed through a Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2), it delivers approximately 5× lower particulate counts than conventional 0.22 µm–filtered media. Formulation: [+] 2 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate | [-] Phenol Red.

Glucose source: 2000 mg/L (2.0 g/L)
Glutathione (reduced) included — antioxidant support for redox-sensitive cell types
0.04 µm final nano-filtration — sub-mycoplasma purity; < 0.05 EU/mL endotoxin
Quadruple-stage filtration: 0.1 µm pre-I → 0.04 µm pre-II → 0.1 µm sterile-I → 0.04 µm final polish
Ultrapure Type 1 water (18.2 MΩ·cm), ISO 13485:2016 QMS, ISO Class 5 fill

CAT. NO.

DCP-RPMI-R1X | UNSPSC 41122100 Cell Culture Media

RPMI 1640 — 1X Liquid

Media familyRPMI 1640
Glucose2000 mg/L (2.0 g/L)
Formulation[+] 2 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate, [-] Phenol Red
AppearanceOrange-colored, clear solution
pH (USP <791>)7.4
Osmolality (USP <785>)280.00–320.00 mOsm/kg H₂O
Endotoxin (USP <85>)< 0.05 EU/mL
Filtration0.1 µm ×2 + 0.04 µm ×2
Shelf Life12 months / 2–8°C

ISO 13485:2016 USP <85> <785> <788> RUO

Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered RPMI passes mycoplasma, subvisible particulates, and endotoxin fragments that activate TLR4 and confound immune cell assays. FluxMPS™ eliminates these failure modes.

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Microchannel-safe purity

0.04 µm final filtration; USP <788> particulate compliance. Particle-free media for suspension immune cell OoC and flow cytometry.

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Immune cell–optimized formulation

RPMI 1640 contains glutathione (antioxidant) and a balanced profile of vitamins and amino acids optimized for lymphocyte and hematopoietic cell culture.

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Ultrapure-grade water

18.2 MΩ·cm — eliminates ionic contaminants that alter lymphocyte signaling thresholds.

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Below TLR4 endotoxin threshold

< 0.05 EU/mL — prevents LPS-driven T cell and NK cell activation artefacts in immune assays.

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Rich nutrient profile

RPMI contains specialized nutrients for demanding cell types — delivered particle-free for OoC use.

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Customization on demand

pH, nutrient concentrations, HEPES, and component modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions.

1

0.1 µm Pre-filtration I — Large Particulate Removal

Removes large aggregates; protects downstream 0.04 µm membranes.
2

0.04 µm Pre-filtration II — Mycoplasma Barrier

Retains mycoplasma (0.1–0.3 µm) — absent from standard 0.22 µm filtration.
3

0.1 µm Sterile-filtration I — Second-pass Redundancy

Second-pass sterility redundancy; no breakthrough from Stage 1.
4

0.04 µm Sterile-filtration II — Final Polish

Ultimate sub-mycoplasma polish; ISO Class 5 aseptic fill & finish.

Performance vs. conventional media

FluxMPS™ DCP-RPMI-R1X delivers approximately 5× lower particulate counts than standard 0.22 µm filtered RPMI, with confirmed mycoplasma barrier at every production stage.

5×

Cleaner than 0.22 µm media by particulate count

0.04

µm Final pore size — sub-mycoplasma polishing

Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma-negative by 0.04 µm barrier every lot.

FluxMPS-Quadruple-stage-filtration-system-Cell-Culture-Media-Diagnocine-V2.jpg — **Figure 1.** FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-RPMI-R1X

Applications

Immune cell OoC and hematopoietic applications

FluxMPS™ DCP-RPMI-R1X — RPMI 1640 — delivers 0.04 µm filtered purity for Hematopoietic cells and related OoC applications.

Automated Bioreactors & Robotics

Next-Generation System Uptime

Optional 0.01 µm (10 nm) ultra-filtered variant available on request.

Total Particulate Exclusion: 10 nm filtration removes nanoparticulate aggregates
Valve & Sensor Protection: Eliminates micro-fouling in automated perfusion systems
Extended Perfusion Stability: Consistent nutrient delivery over long-duration culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm variant.

Immunology

T Cell & Lymphocyte Culture

RPMI 1640 is the standard for primary T cells, B cells, NK cells, and monocytes. FluxMPS 0.04 µm filtration prevents particulate TLR4 activation that confounds immune assays.

T cellsB cellsNK cellsPBMC

Cancer Biology

Leukemia & Lymphoma Lines

RPMI supports NCI-60 cancer lines, Jurkat, Raji, K562, HL-60, and all hematopoietic cancer lines where DMEM would alter proliferation and signaling.

JurkatRajiK562HL-60

Microfluidics

Immune Cell OoC

0.04 µm filtered RPMI for tumor-immune interaction chips, vascular-immune OoC, and lymph node-on-chip where particle-free media prevents TLR4 activation.

Tumor-immune chipLymph node OoCMPS

Immunotherapy

CAR-T & TIL Expansion

Ultra-low endotoxin (<0.05 EU/mL) prevents LPS-driven T cell activation artefacts in CAR-T manufacturing and TIL expansion protocols.

CAR-TTILTCR-T

Metabolomics

Immune Cell Metabolic Flux

Defined RPMI base for Seahorse XF T cell metabolic assays, glycolysis stress tests, and ¹³C isotope tracing of lymphocyte activation states.

Seahorse XF¹³C tracingGlycolysis

Live-Cell Imaging

Flow Cytometry & Confocal

Ultra-low particulate; phenol red–free variants eliminate autofluorescence for PE-channel flow cytometry and immune cell confocal imaging.

Flow cytometryConfocalELISA

Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters

Parameter	Specification
Formulation	[+] 2 g/L Glucose, [+] L-Glutamine, [+] Sodium Pyruvate, [+] Sodium Bicarbonate \| [-] Phenol Red
Appearance	Orange-colored, clear solution
Glucose	2000 mg/L (2.0 g/L)
pH USP <791>	7.4
Osmolality USP <785>	280.00–320.00 mOsm/kg H₂O
Total ingredients	40

Sterility, Purity & Safety

Parameter	Specification
Endotoxin USP <85> BET	< 0.05 EU/mL
Sterility USP <71>	No growth / 14 days
Mycoplasma	Negative (0.04 µm barrier)
Particulate ≥10 µm USP <788>	NMT 25/mL
Particulate ≥25 µm USP <788>	NMT 3/mL
Water purity	Type 1, 18.2 MΩ·cm
Manufacturing std.	ISO 13485:2016
Fill environment	ISO Class 5 (Class 100)

Storage, Handling & Logistics

Parameter	Specification
Storage temperature	2–8°C, away from light
Freeze-thaw	Do not freeze
Shelf life	12 months from manufacture
Shipping condition	cold pack
CO₂ requirement	5% CO₂ required (sodium bicarbonate buffering)

Raw Materials & Regulatory

Parameter	Specification
Raw material grade	Reagent / cell culture grade
Traceability	Full lot traceability per ISO 13485
Manufacturing QMS ISO	ISO 13485:2016 certified
Regulatory alignment	21 CFR Part 820 (cGMP) aligned
Production method	Micro-batch, per-lot QC release
Intended use	Research Use Only (RUO)

Formulation

Full composition (mg/L)

RPMI 1640: 40 ingredients verified per lot with CAS numbers for full raw-material traceability. RPMI 1640 contains glutathione (antioxidant) and a balanced profile of vitamins and amino acids optimized for lymphocyte and hematopoietic cell culture.

Component	CAS Number	mg/L
INORGANIC SALTS
Calcium nitrate tetrahydrate	13477-34-4	100.000
Magnesium sulphate anhydrous	7487-88-9	48.840
Potassium chloride	7447-40-7	400.000
Sodium bicarbonate	144-55-8	2000.000
Sodium chloride	7647-14-5	6000.00
Sodium phosphate dibasic anhydrous	7558-79-4	800.000

Component	CAS Number	mg/L
AMINO ACIDS
Glycine	56-40-6	10.000
L-Arginine hydrochloride	1119-34-2	241.000
L-Asparagine	70-47-3	50.000
L-Aspartic acid	56-84-8	20.000
L-Cystine dihydrochloride	30925-07-6	65.200
L-Glutamic acid	56-86-0	20.000
L-Glutamine	56-85-9	300.000
L-Histidine hydrochloride monohydrate	5934-29-2	20.960
L-Hydroxyproline	51-35-4	20.000
L-Isoleucine	73-32-5	50.000
L-Leucine	61-90-5	50.000
L-Lysine hydrochloride	657-27-2	40.000
L-Methionine	63-68-3	15.000
L-Phenylalanine	63-91-2	15.000
L-Proline	147-85-3	20.000
L-Serine	56-45-1	30.000
L-Threonine	72-19-5	20.000
L-Tryptophan	73-22-3	5.000
L-Tyrosine Disodium Salt	69847-45-6	28.830
L-Valine	72-18-4	20.000

Component	CAS Number	mg/L
VITAMINS
Choline chloride	67-48-1	3.000
D-Biotin	58-85-5	0.200
D-Ca-Pantothenate	137-08-6	0.250
Folic acid	59-30-3	1.000
Niacinamide	98-92-0	1.000
Pyridoxine hydrochloride	58-56-0	1.000
Riboflavin	83-88-5	0.200
Thiamine hydrochloride	67-03-8	1.000
Vitamin B12	68-19-9	0.005
i-Inositol	87-89-8	35.000
p-Amino benzoic acid (PABA)	150-13-0	1.000
OTHERS
D-Glucose	50-99-7	2000.000
Glutathione reduced	70-18-8	1.000
Sodium Pyruvate	113-24-6	110.000

Custom formulation: Contact support@diagnocine.com for DCP-RPMI-R1X modifications.

Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016–certified and CE-approved facilities. Final QA at DiagnoCine R&D Center, Totowa, NJ, USA.

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Ultrapure Type 1 Water

18.2 MΩ·cm — eliminates ionic contaminants.

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ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

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Micro-Batch Precision

Small-batch, per-lot tested — no blending, Certificate of Analysis for every lot.

Endotoxin — USP <85> BET

LAL assay < 0.05 EU/mL per lot.

Particulate — USP <788> Method 2

≤25/mL (≥10 µm), ≤3/mL (≥25 µm).

Osmolality — USP <785>

Target: 280.00–320.00 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available on request.

Certificate of Analysis: Request for any DCP-RPMI-R1X lot at support@diagnocine.com.

Product Comparison

How DCP-RPMI-R1X compares

FluxMPS™ DCP-RPMI-R1X vs. conventional 0.22 µm–filtered RPMI formulations.

Parameter	DCP-RPMI-R1X (FluxMPS™)	Conventional RPMI 1640 (0.22 µm filtered)	Standard DMEM/RPMI (0.22 µm filtered)
RPMI 1640 without Phenol Red — autofluorescence-free for flow cytometry, confocal, and hormone-sensitive assays	check_circle Yes	cancel No	cancel No
Final filtration pore size	0.04 µm	0.22 µm	0.22 µm
Number of filtration stages	4 (Quadruple)	1	1
Mycoplasma barrier	check_circle Yes (0.04 µm)	cancel No	cancel No
Endotoxin specification	< 0.05 EU/mL	NMT 1 EU/mL	NMT 1 EU/mL
USP <788> particulate tested	check_circle Yes	cancel No	cancel No
Water quality	Type 1, 18.2 MΩ·cm	Purified water	Purified water
Manufacturing QMS	ISO 13485:2016	ISO 9001 or none	ISO 9001 or none
Microfluidic channel compatible	check_circle MPS-grade	cancel Risk of clogging	cancel Risk of clogging

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-RPMI-R1X — RPMI 1640.

DCP-RPMI-R1X is processed through a Quadruple-stage filtration system reaching 0.04 µm final pore size. RPMI 1640 with sub-mycoplasma filtration purity is suited to immune cell OoC, tumor-immune interaction chips, and lymphocyte perfusion models where standard RPMI would cause particulate TLR4 activation.

FluxMPS™ uses four sequential filters — 0.1 µm pre-filtration I, 0.04 µm pre-filtration II (mycoplasma barrier), 0.1 µm sterile-filtration I, and 0.04 µm final polish — resulting in approximately 5× fewer particles and confirmed mycoplasma retention at every production stage.

Phenol red is removed to eliminate autofluorescence at 520–560 nm and its weak estrogenic activity. Essential for lymphocyte flow cytometry (PE channel interference), immune cell confocal imaging, hormone-sensitive cancer cell assays (ER+ breast cancer, endocrine tumors), and any ELISA-based assay where phenol red absorbs at relevant wavelengths.

5% CO₂ required (sodium bicarbonate buffering).

Yes. Add FBS (5–10%), serum-free supplements, growth factors, or antibiotics as required. Contact support@diagnocine.com for custom co-formulation.

FluxMPS™ DCP-RPMI-R1X is produced to meet < 0.05 EU/mL by LAL assay (USP <85>). For immune cells, endotoxin above 0.1 EU/mL activates TLR4/NF-κB, inducing cytokines and altering lymphocyte activation state independently of experimental conditions.

Yes. Full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma, particulate count (USP <788> Method 2), and raw-material traceability. Request at support@diagnocine.com.

Scientific References

Supporting literature

Key publications supporting RPMI 1640 in immune cell culture and OoC applications.

Moore GE, et al. Culture of normal human leukocytes. JAMA. 1967;199:519–524. doi:10.1083/jcb.1.3.273
Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
Jang KJ, et al. Human kidney proximal tubule-on-a-chip. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
Sung JH, et al. Microfabricated mammalian organ systems. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

FluxMPS™ RPMI 1640 Medium w/o Phenol Red: 1X Liquid