Tris Acetate EDTA Buffer [20X]
Cat No.: DCP-TAE20X
Size: 500 mL & 1 L
Non-Sterile; Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Tris Acetate EDTA (TAE) Buffer is commonly used as a running buffer for preparing agarose gels in DNA electrophoresis. Alkaline pH keeps the DNA molecules deprotonated and negatively charged, allowing their migration toward the positive electrode during electrophoresis. Specifically, it ensures that the phosphate groups on the DNA backbone are deprotonated, giving the DNA molecules an overall negative charge. The negatively charged DNA molecules migrate through the agarose gel matrix. Smaller DNA fragments can move more easily through the gel's pores than larger fragments, resulting in size-based separation. The acetate ions help maintain the buffering capacity and conductivity of the buffer system during electrophoresis. It helps prevent the degradation of DNA by nucleases.
Advantages:
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It provides better resolution and separation of larger DNA fragments.
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Faster double-stranded DNA migration is achieved through agarose gels.
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It does not interfere with the activity of enzymes used in cloning procedures.
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DNA extraction: TAE works better for performing DNA extraction from agarose gels after electrophoresis.
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Long-duration electrophoresis runs: While TAE has a lower buffering capacity than TBE, it can be used for extended electrophoresis by periodically replacing or circulating the buffer.
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Polyacrylamide gel electrophoresis: Although less common, TAE can also be used for polyacrylamide gel electrophoresis of nucleic acids
Applications:
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Agarose gel electrophoresis of DNA and RNA.
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Denaturing gradient gel electrophoresis for mutation analysis.
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Studying DNA mobility in solution with or without added salts like NaCl.
Composition:
| Ingredients |
Concentration |
| Tris base |
800 mM |
| EDTA |
20 mM |
| Glacial acetic acid |
400 mM |
Quality Control:
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Appearance: Clear, Colorless Liquid.
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pH: 8.0
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Sterility: Filtered 0.1 micron Twice and 0.04 micron once in a non-sterile environment.
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DNase activity: None was detected after incubation of plasmid DNA and this product for 18 hr at room temperature.
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RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
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Storage: 4 ℃
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Shelf life: 2 years
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA
