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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid

Product#: DCP-DMEMH-QB1X
$44.00
DCP-DMEMH-QB1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid

Contains Phenol Red Contains 25mM HEPES Contains Calcium Contains Magnesium Contains High Glucose (4500 mg/L) Contains Sodium Pyruvate Without L-Glutamine Without Sodium Bicarbonate

FluxMPS™ DCP-DMEMH-QB1X is a Microfluidics Suitable, ultra-filtered DMEM High Glucose (4.5 g/L) formulation with 25 mM HEPES buffer, engineered for organ-on-a-chip (OoC), microphysiological systems (MPS), and CO₂-independent or open-top microfluidic platforms. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. HEPES (25 mM, pKa 7.3 at 37°C) provides robust CO2-independent pH buffering. Formulation: [+] Phenol Red, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] High Glucose (4500 mg/L), [+] Sodium Pyruvate | [-] L-Glutamine, [-] Sodium Bicarbonate.

  • High Glucose (4.5 g/L) — supports neurons, cardiomyocytes, cancer lines, and aerobically active cells
  • 25 mM HEPES (pKa 7.3 at 37°C) — robust pH buffering independent of CO2 tension
  • 0.04 µm final filtration — sub-mycoplasma-size pore rating for microfluidic channels below 100 µm
  • Quadruple-stage filtration: Prefiltration I (0.1 µm) → Final filtration I (0.04 µm) → Prefiltration II (0.1 µm) → Final filtration II — Polish (0.04 µm)
  • Endotoxin release specification < 0.05 EU/mL (LAL, USP <85>), controlled per manufacturing batch
  • Contains phenol red (15.9 mg/L) and is formulated without L-glutamine or sodium bicarbonate for HEPES-only, CO2-independent buffering
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Custom pH, salts, glucose, HEPES concentration, and nutrient adjustments available on request
CAT. NO.
DCP-DMEMH-QB1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco's Modified Eagle Medium (DMEM), High Glucose & 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid
  • Glucose4500 mg/L (4.5 g/L, High Glucose)
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • Formulation[+] Phenol Red, [+] HEPES, [+] Calcium, [+] Magnesium, [+] High Glucose, [+] Sodium Pyruvate, [-] L-Glutamine, [-] Sodium Bicarbonate
  • AppearanceRed to orange-red, clear solution (phenol red indicator present)
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)310–350 mOsm/kg H2O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, away from light
  • Shelf Life12 months from date of manufacture, unopened
Available sizes: 500 mL, 1000 mL
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm–filtered DMEM passes mycoplasma-sized organisms, subvisible particulates, and protein aggregates that clog microfluidic channels. Standard DMEM also lacks HEPES buffering, causing pH instability whenever chips are handled outside incubators. FluxMPS™ addresses both failure modes simultaneously.

filter_alt

Microchannel-safe purity

0.04 µm final filtration and USP <788> particulate compliance support safe perfusion across a wide range of chip geometries, including sub-100 µm channels.

bolt

High-energy cell support

4.5 g/L glucose sustains energy-demanding cell types — primary neurons, iPSC-derived cardiomyocytes, Warburg-active cancer lines — in long-duration perfusion.

water_drop

HEPES: CO2-stable pH

25 mM HEPES (pKa 7.3 at 37°C) maintains pH 7.2–7.4 regardless of CO2 fluctuation — useful for open-top chips, point-of-care devices, and atmospheric incubation.

visibility

Low background for imaging

Ultra-low particulate baseline supports confocal microscopy and biosensor platforms on chip; particulate control is independent of phenol red content, which is present in this formulation.

science

Rich, stable nutrient profile

32 ingredients verified per lot; 4× BME amino acid/vitamin concentrations; micro-batch production with full traceability.

tune

Customization on demand

pH, glucose, HEPES concentration, salts, and nutrients adjustable. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions — delivering sub-mycoplasma-size pore rating unavailable from conventional 0.22 µm media.

  1. 1

    0.1 µm Prefiltration I

    Removes large aggregates, cell debris, and protein aggregates; protects the first 0.04 µm final filter cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and mycoplasma-sized organisms (0.2–0.3 µm) that pass a standard 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated pre-filter, protecting the second 0.04 µm final filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; ISO Class 5 aseptic fill & finish.

Performance vs. conventional media

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size, four filtration passes
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>); mycoplasma risk is mitigated by 0.1 µm mycoplasma-retentive filtration at every production stage (not tested per lot).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ DCP-DMEMH-QB1X Dulbecco's Modified Eagle Medium (DMEM), High Glucose and 25mM HEPES w/o L-Glutamine, Sodium Bicarbonate: 1X Liquid ? Quadruple-stage filtration system: 0.1 micron Prefiltration I, 0.04 micron Final filtration I (mycoplasma barrier), 0.1 micron Prefiltration II, 0.04 micron Final filtration II Polish ? Microfluidics Suitable DMEM High Glucose HEPES for organ-on-a-chip | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2) for sub-mycoplasma-size pore rating.
© Diagnocine® — DCP-DMEMH-QB1X
Applications

Designed for next-generation cell models

FluxMPS™ DCP-DMEMH-QB1X combines high-glucose energy support with HEPES pH stability — supporting demanding platforms from open-top microfluidic chips and CO2-free bioreactors to multi-organ body-on-a-chip systems.

Automated Bioreactors & Robotics

Next-Generation System Uptime

HEPES buffering reduces CO2 dependency in automated bioreactor perfusion. An optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant is available on request for robotic liquid handlers where even trace particulates cause valve failure.

  • Low Particulate Delivery: Quadruple-stage 0.04 µm filtration reduces particulate load in bioreactor media lines
  • pH-Stable Automated Delivery: HEPES maintains pH during robotic media exchanges without CO2 re-equilibration delays
  • Extended Perfusion Stability: Consistent high-glucose delivery with stable pH over weeks-long culture

Inquiry Required: Contact support@diagnocine.com for the 0.01 µm MPS Grade variant.

Microfluidics

Open-Top & CO2-Free Chips

HEPES buffering enables pH-stable culture in open-top microfluidic devices, atmospheric incubators, and multi-compartment chips with heterogeneous CO2 environments.

OoCToCBoCLoCMPS
Neuroscience

Primary Neurons & Brain-on-Chip

High glucose (4.5 g/L) plus HEPES stability supports primary neurons and iPSC-neuronal networks in long-duration microfluidic perfusion without pH drift.

iPSC-NeuronsPrimary neuronsBrain-on-chip
Cardiac Biology

Cardiomyocyte & Heart-on-Chip

High-glucose DMEM plus HEPES is a common base for iPSC-CM maturation and heart-on-chip functional assays requiring stable pH and high energy substrate.

iPSC-CMHeart-on-chipTEER
Cancer Biology

Warburg Effect & Cancer Models

High glucose supports aerobic glycolysis in cancer lines; HEPES stabilizes pH during rapid glucose consumption spikes in Warburg-active tumour models.

MCF-7MDA-MB-231HeLaA549
Metabolomics

Metabolic Flux Analysis

Defined high-glucose formulation supports 13C isotope tracing and NMR metabolomics; HEPES allows sampling outside incubators without pH artefacts. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium — this formulation contains phenol red.

¹³C tracingNMR metabolomics
Live-Cell Imaging

Microscopy & Optical Sensing

HEPES-stabilized pH during imaging sessions supports confocal microscopy and biosensor platforms on chip.

ConfocalBiosensorsTEER
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Phenol Red, [+] HEPES (25 mM), [+] Calcium, [+] Magnesium, [+] High Glucose (4500 mg/L), [+] Sodium Pyruvate | [-] L-Glutamine, [-] Sodium Bicarbonate
Appearance Red to orange-red, clear solution (phenol red indicator present)
Glucose 4500 mg/L (4.5 g/L, High Glucose)
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
pH USP <791> 7.4
Osmolality USP <785> 310–350 mOsm/kg H2O
Total ingredients 32 components across 4 categories (Inorganic Salts, Amino Acids, Vitamins, Others)
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release specification; see §Quality Assurance)
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> NMT 25/mL
Particulate ≥25 µm USP <788> NMT 3/mL
Water purity Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, away from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO2 requirement CO2-independent — HEPES (25 mM) alone maintains pH 7.2–7.4 at 37°C without gas supplementation
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

DMEM High Glucose + HEPES is a modification of Basal Medium Eagle (BME) with 4× BME amino acid and vitamin concentrations. 32 ingredients verified per lot with CAS numbers for full raw-material traceability. HEPES (25 mM = 5958 mg/L) is listed in the Others group.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
OTHERS
i-Inositol 87-89-8 7.200
D-Glucose 50-99-7 4500.000
Phenol red sodium salt 34487-61-1 15.900
Sodium pyruvate 113-24-6 110.000
HEPES 7365-45-9 5958.000
Custom formulation: Contact support@diagnocine.com for DCP-DMEMH-QB1X custom specifications — pH, glucose, HEPES concentration, salts, or nutrient modifications.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system spanning raw materials, in-process controls, and final-product testing.

verified

ISO 13485:2016 Quality Management

Manufactured under ISO 13485:2016-certified facilities. Final QA and testing at Diagnocine R&D Center, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm resistivity Type 1 water with trace-metal and organic-carbon (TOC) control for consistent raw-material quality.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations, supporting container-closure integrity.

assignment

Micro-Batch Precision

Small-batch production, full per-lot traceability, Certificate of Analysis for every lot — no blending, no averaged QC results.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL per batch.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Freezing-point osmometry. Target: 310–350 mOsm/kg H2O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-DMEMH-QB1X compares

FluxMPS™ DCP-DMEMH-QB1X vs. conventional 0.22 µm–filtered DMEM High Glucose formulations.

Parameter DCP-DMEMH-QB1X (FluxMPS™) Conventional DMEM HG
(0.22 µm filtered)		 Standard DMEM HG + HEPES
(0.22 µm filtered)
Grade Microfluidics Suitable Not specified Not specified
Distinctive formulation: HEPES-only, CO2-independent High Glucose DMEM (fresh glutamine added at use) check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1/0.04 µm) cancel No cancel No
HEPES buffer (25 mM) check_circle Yes cancel No check_circle Yes
Endotoxin specification < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance tested check_circle Yes cancel No cancel No
Water quality Type 1, 18.2 MΩ·cm Purified water Purified water
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Microfluidic channel compatible check_circle Microfluidics Suitable cancel Risk of clogging cancel Risk of clogging
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEMH-QB1X DMEM High Glucose + 25mM HEPES.

Yes. DCP-DMEMH-QB1X combines Quadruple-stage 0.04 µm filtration for a low-particulate baseline in microfluidic channels with 25 mM HEPES buffering for CO2-stable pH. High glucose (4.5 g/L) supports energy-demanding cell types — neurons, cardiomyocytes, and cancer lines — in long-duration chip perfusion. The HEPES component is particularly valuable for open-top chips where CO2 concentration fluctuates.
 
This formulation is CO2-independent (HEPES alone buffers pH without bicarbonate) and is intended for fresh L-glutamine addition at the time of use (typically 2 mM), since glutamine degrades in liquid storage over time. It is ideal for atmospheric-incubation, open-top chip platforms requiring precise nitrogen control and no CO2 dependency — a common requirement for multi-compartment body-on-a-chip devices.
No. This medium is CO2-independent — HEPES (25 mM) alone maintains pH 7.2–7.4 at 37°C without gas supplementation. HEPES (pKa 7.3 at 37°C) maintains stable pH across a broad CO2 range, making this medium suitable for open-top chips, point-of-care platforms, and atmospheric incubation where CO2 control is impractical.
Yes. FBS (typically 5–10%), serum-free supplements, growth factors, antibiotics, or custom nutrients may be added as required. Filter serum-containing or protein-containing additions through a 0.2 µm low-protein-binding PES or PVDF membrane before use; defined, protein-free additions may use a 0.1 µm membrane. Never use a 0.04 µm membrane for supplement filtration — it will strip serum proteins and clog rapidly. Contact support@diagnocine.com for custom co-formulation.
Endotoxin is controlled per manufacturing batch, not per individual unit. Every batch is tested by LAL assay (USP <85>, Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL) before release and must meet the release specification of < 0.05 EU/mL. A Certificate of Analysis is available on request.
Yes. A full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key peer-reviewed publications supporting Microfluidics Suitable, HEPES-buffered, ultra-filtered DMEM High Glucose in organ-on-a-chip and metabolic research.

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
  4. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x
  5. Katt ME, et al. In vitro tumor models: advantages, disadvantages, variables, and selecting the right platform. Front Bioeng Biotechnol. 2016;4:12. doi:10.3389/fbioe.2016.00012
  6. Jang KJ, et al. Human kidney proximal tubule-on-a-chip for drug transport and nephrotoxicity assessment. Integr Biol. 2013;5:1119–1129. doi:10.1039/c3ib40049b
  7. Schimek K, et al. Integrating biological vasculature into a multi-organ-chip microsystem. Lab Chip. 2013;13:3588–3598. doi:10.1039/c3lc50217a
  8. Luni C, et al. High-efficiency cellular reprogramming with microfluidics. Nat Methods. 2016;13:446–452. doi:10.1038/nmeth.3832
  9. Sung JH, et al. Microfabricated mammalian organ systems and their integration into models of whole animals and humans. Lab Chip. 2013;13:1201–1212. doi:10.1039/c3lc41017j

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