0
Precision Quality to Your Research verified ISO 13485 Certified | SBA WOSB | NJ MWBE · NYC MWBE · Illinois BEP arrow_forward View Details

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Product#: DCP-DMEMH-GQPB1X
$44.00
DCP-DMEMH-GQPB1X
Availability:
Ships in 1-2 Weeks

warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid

Contains Phenol Red Contains HEPES (25 mM) Contains Calcium Contains Magnesium Without L-Glutamine Without Sodium Bicarbonate Without Glucose Without Sodium Pyruvate

FluxMPS™ DCP-DMEMH-GQPB1X is a Microfluidics Suitable, ultra-filtered glucose-free DMEM with 25 mM HEPES, engineered for metabolic flux analysis, carbon-13 isotope tracing, Warburg-effect research, and organ-on-a-chip applications where carbon source identity must be precisely controlled. A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration. Formulation: [+] Phenol Red, HEPES (25 mM), Calcium, Magnesium | [-] Glucose, L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate.

  • Glucose-free — add D-glucose, ¹³C-glucose, galactose, fructose, or any carbon source at your defined concentration
  • 25 mM HEPES (pKa 7.3 at 37°C) — CO₂-independent pH stability for open-top chips and metabolic sampling outside incubators
  • 0.04 µm final nano-filtration — retains particulates in the mycoplasma size range (0.2–0.3 µm); endotoxin release specification < 0.05 EU/mL
  • Quadruple-stage filtration: 0.1 µm prefilter I → 0.04 µm final filter I → 0.1 µm prefilter II → 0.04 µm final filter II (polish)
  • Contains phenol red (15.9 mg/L) as a pH indicator — not compatible with Agilent Seahorse XF assays, which require phenol-red-free medium; ask about our phenol-red-free variant
  • No glucose, L-glutamine, sodium pyruvate, or sodium bicarbonate added — every carbon and nitrogen input is researcher-defined
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Custom glucose concentrations, carbon sources, and nutrient modifications available on request
Incomplete basal medium. This formulation contains no glucose and no sodium pyruvate. A carbon source, serum, or defined supplements must be added before use. See the FAQ for supplementation guidance.
CAT. NO.
DCP-DMEMH-GQPB1X | Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate: 1X Liquid
  • GlucoseNot added — researcher-defined
  • HEPES25 mM (5958 mg/L), pKa 7.3 at 37°C
  • Formulation[+] Phenol Red, HEPES, Calcium, Magnesium | [-] Glucose, L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate
  • AppearanceOrange-colored, clear solution
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)310–350 mOsm/kg H₂O
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Storage2–8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

When carbon source control is the experiment

Standard DMEM contains a fixed glucose concentration that cannot be changed without switching media entirely. FluxMPS™ glucose-free DMEM + HEPES gives you a clean base formulation where every carbon source is added fresh at the concentration and identity you choose, while ultra-low particulate delivery supports chip integrity and a < 0.05 EU/mL endotoxin release specification.

science

Complete carbon source control

Add D-glucose, ¹³C-glucose, U-¹³C?-galactose, fructose, or no sugar. Combine with palmitate or other fatty acids. Define carbon source identity and concentration precisely for every experiment.

analytics

Metabolic flux & isotope tracing ready

Glucose-free base with no pyruvate background supports ¹³C-glucose tracing, Warburg-vs-OXPHOS switching studies, and NMR/LC-MS metabolomics without dilution from residual carbon sources. Contains phenol red; not suitable for Agilent Seahorse XF assays, which require a phenol-red-free base — a phenol-red-free variant is available on request.

water_drop

HEPES: CO₂-stable pH

25 mM HEPES (pKa 7.3 at 37°C) maintains pH outside CO₂ incubators — useful for metabolic sampling and open-top chip platforms.

filter_alt

Microchannel-safe purity

0.04 µm final filtration and a < 0.05 EU/mL endotoxin release specification are intended to reduce particulate and endotoxin-driven confounds in metabolic baseline measurements.

visibility

Low background for imaging

This formulation contains phenol red (15.9 mg/L) as a pH indicator, which contributes assay background fluorescence; for autofluorescence-sensitive confocal or biosensor imaging, request the phenol-red-free variant. The 0.04 µm final filtration nonetheless provides an ultra-low particulate baseline in this formulation.

tune

Customization on demand

Custom glucose concentrations, carbon source additions, pH, and nutrient modifications available. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Four serial filtration stages reaching a final 0.04 µm polish under ISO Class 5 aseptic conditions — two paired prefilter + final-filter passes run in series, each 0.04 µm final filter protected by its own dedicated 0.1 µm prefilter.

  1. 1

    0.1 µm Prefiltration I

    Large particulate, cell debris & protein aggregate removal; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter, including the size range associated with mycoplasma (0.2–0.3 µm).

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish under ISO Class 5 (Class 100) conditions.

Why filtration purity matters for metabolic assays

Subvisible particulates and endotoxin above assay-relevant thresholds can independently activate TLR4/NF-κB signalling in some cell systems, confounding glucose uptake, lactate production, and OCR measurements. FluxMPS™ DCP-DMEMH-GQPB1X is filtered to a 0.04 µm final pore size across four passes and released to an endotoxin specification of < 0.05 EU/mL.

5×
Cleaner than 0.22 µm media by particulate count
0.04
µm Final pore size across 4 filtration passes
Sterility & Mycoplasma: No growth after 14-day incubation (USP <71>). Mycoplasma control is achieved by 0.1 µm and 0.04 µm membrane filtration, retentive against organisms in the 0.2–0.3 µm size range; this is a filtration statement, not a per-lot mycoplasma assay (USP <63>).
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS DCP-DMEMH-GQPB1X Dulbecco's Modified Eagle Medium DMEM 25mM HEPES glucose-free L-glutamine-free sodium-pyruvate-free sodium-bicarbonate-free 1X liquid, Quadruple-stage filtration system 0.1 micron prefiltration I, 0.04 micron final filtration I, 0.1 micron prefiltration II, 0.04 micron final filtration II polish, Microfluidics Suitable cell culture medium for organ-on-a-chip and microfluidic applications, Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration system (0.1 µm ×2 + 0.04 µm ×2).
© Diagnocine® — DCP-DMEMH-GQPB1X
Applications

Metabolic research and OoC applications

FluxMPS™ DCP-DMEMH-GQPB1X is built for experiments where carbon source identity must be controlled — from ¹³C metabolic flux analysis to Warburg-vs-OXPHOS switching and glucose-dose response studies on organ-on-a-chip platforms.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated, closed-loop bioreactor and robotic liquid-handling platforms where microvalve and sensor fouling drive unplanned downtime, Diagnocine offers an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation — a distinct product tier from the 0.04 µm Microfluidics Suitable grade described on this page (see the Grade note in the filtration section above).

  • Total particulate exclusion: 0.01 µm cut-off removes essentially all particulate matter above nanometer scale
  • Valve & sensor protection: reduces fouling of microvalves, flow sensors, and optical windows in automated systems
  • Extended perfusion stability: supports longer unattended perfusion runs between maintenance cycles

Inquiry Required: The 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com to request this formulation.

Metabolomics

¹³C Metabolic Flux Analysis

Add U-¹³C?-glucose at any specific activity without background dilution. HEPES maintains pH during NMR sample preparation outside incubators.

¹³C tracingNMR metabolomicsLC-MS/MS
Cancer Biology

Warburg Effect & OXPHOS Switching

Add glucose (Warburg), galactose (force OXPHOS), or no sugar (starvation) to study metabolic flexibility in cancer lines without media changes.

MCF-7MDA-MB-231HeLaA549
Microfluidics

Glucose Dose-Response on Chip

Perfuse defined glucose gradients through OoC channels to study dose-dependent metabolic responses in endothelial, beta-cell, or hepatocyte chips.

OoCToCDiabetes-on-chipLiver-on-chip
Stem Cell Biology

iPSC Metabolic Maturation

Glucose-free base enables galactose-forced OXPHOS maturation of iPSC-derived cardiomyocytes and hepatocytes, improving metabolic phenotype fidelity.

iPSC-CMiPSC-HepiPSC-β cells
Nutrient Biology

Nutrient Deprivation Studies

Starvation experiments, glucose withdrawal, and nutrient re-feeding protocols with defined timing and concentration control.

AutophagymTOR signalingAMPK activation
Live-Cell Imaging

Metabolic Imaging & Biosensors

Request the phenol-red-free variant of this formulation for FLIM, NAD(P)H imaging, glucose FRET sensors, and TEER measurements where phenol red autofluorescence must be minimized.

FLIMGlucose sensorsTEERConfocal
Technical Specifications

Analytical release specifications

Every lot released against the full specification matrix. CoA: support@diagnocine.com.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Phenol Red, HEPES (25 mM), Calcium, Magnesium | [-] Glucose, L-Glutamine, Sodium Pyruvate, Sodium Bicarbonate
Appearance Orange-colored, clear solution
Glucose Not added — add at desired concentration
HEPES 25 mM (5958 mg/L), pKa 7.3 at 37°C
pH USP <791> 7.4
Osmolality USP <785> 310–350 mOsm/kg H₂O
Total ingredients 30
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL
Sterility USP <71> No growth / 14 days
Mycoplasma 0.1 & 0.04 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm USP <788> Method 1 NMT 25/mL
Particulate ≥25 µm USP <788> Method 1 NMT 3/mL
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8°C, protect from light
Freeze-thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping condition Cold pack
CO₂ requirement CO₂-independent — HEPES (25 mM) alone maintains pH without gas supplementation
Pack sizes available 500 mL, 1000 mL
Raw Materials & Regulatory
Parameter Specification
Raw material grade Reagent / cell culture grade
Traceability Full lot traceability per ISO 13485
Manufacturing QMS ISO ISO 13485:2016 certified
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch, per-lot QC release
Intended use Research Use Only (RUO)
Formulation

Full composition (mg/L)

Glucose-free DMEM + HEPES scaffold: 30 ingredients verified per lot with CAS numbers. Glucose is intentionally absent — add your carbon source of choice. HEPES (25 mM = 5958 mg/L, CAS 7365-45-9) and phenol red are listed under OTHERS.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-15-0 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
OTHERS
i-Inositol 87-89-8 7.200
Phenol red sodium salt 34487-61-1 15.900
HEPES 7365-45-9 5958.000
Carbon source supplementation: Glucose is not included. For standard culture add D-glucose (0.1 µm sterile-filtered stock, protein-free) at 1000 mg/L (low) or 4500 mg/L (high). For ¹³C tracing add U-¹³C?-glucose. For OXPHOS forcing add D-galactose (2000 mg/L). Serum or protein-containing additions should be filtered through a 0.2 µm low-protein-binding PES or PVDF membrane, not 0.04 µm. Contact support@diagnocine.com for custom carbon source pre-formulations.
Quality Assurance

Manufacturing & compliance

Every FluxMPS™ product is manufactured and released under a rigorous multi-layer quality system.

verified

ISO 13485:2016 Quality Management

Manufactured under an ISO 13485:2016-certified quality management system. Final QC at Diagnocine, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm feed water with low trace-metal and total organic carbon content, used throughout formulation.

biotech

ISO Class 5 Fill & Finish

Aseptic fill in validated ISO Class 5 (Class 100) laminar-flow workstations.

assignment

Micro-Batch Precision

Small-batch, per-lot tested — no blending between batches; Certificate of Analysis available for every lot.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration: NMT 25/mL (≥10 µm), NMT 3/mL (≥25 µm).

Osmolality — USP <785>

Target: 310–350 mOsm/kg H₂O.

Documentation & CoA

Full CoA with raw-material traceability available for every lot on request.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-DMEMH-GQPB1X compares

FluxMPS™ DCP-DMEMH-GQPB1X vs. conventional glucose-free DMEM and standard DMEM for metabolic assays.

Parameter DCP-DMEMH-GQPB1X (FluxMPS™) Conventional GF-DMEM
(0.22 µm filtered)		 Standard DMEM
(fixed glucose, 0.22 µm)
Grade Microfluidics Suitable Not specified Not specified
Formulation excludes glucose, L-glutamine, sodium pyruvate & sodium bicarbonate — all metabolic inputs researcher-defined check_circle Yes cancel No cancel No
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration passes 4 1 1
Mycoplasma-retentive filtration check_circle Yes (0.1 & 0.04 µm) cancel No cancel No
Endotoxin (release specification) < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate tested check_circle Yes cancel No cancel No
Seahorse XF compatible cancel No (contains phenol red; request phenol-red-free variant) check_circle Yes (no HEPES) cancel No (fixed carbon source)
Manufacturing QMS ISO 13485:2016 ISO 9001 or none ISO 9001 or none
Custom formulation check_circle Available cancel Fixed cancel Fixed

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ DCP-DMEMH-GQPB1X glucose-free DMEM + 25 mM HEPES.

Yes. The 0.04 µm final filtration and HEPES-buffered, CO₂-independent formulation are well suited to microfluidic channels and open-top OoC/microphysiological system platforms. Note that this formulation contains phenol red (15.9 mg/L); for optical biosensor or TEER chips sensitive to autofluorescence, request our phenol-red-free variant.
This medium is processed through four filtration passes — 0.1 µm prefilter I, 0.04 µm final filter I, 0.1 µm prefilter II, and 0.04 µm final filter II — reaching a final 0.04 µm pore size, well below the 0.22 µm sterilizing-grade cut-off used by many conventional media. It is released to an endotoxin specification of < 0.05 EU/mL and to USP <788> Method 1 particulate limits.
Removing these four components lets you define every major metabolic input independently. Add D-glucose (0.1 µm sterile-filtered stock) at 1000 mg/L (low) or 4500 mg/L (high); for ¹³C tracing add U-¹³C?-glucose at the same concentration; for OXPHOS forcing replace glucose with D-galactose (2000 mg/L); for fatty acid oxidation studies add a palmitate-BSA conjugate (filter through 0.2 µm low-protein-binding PES/PVDF, not 0.04 µm) with no exogenous sugar. Contact support@diagnocine.com for supplementation guidance.
No. This formulation contains no sodium bicarbonate and is buffered with 25 mM HEPES, so it is CO₂-independent and maintains pH without gas supplementation — useful for open-top chips and metabolic sampling outside incubators.
Yes. Serum and other protein-containing supplements should be sterile-filtered through a 0.2 µm low-protein-binding PES or PVDF membrane before addition — never through a 0.04 µm membrane, which retains IgM, lipoproteins, and much of the functional serum fraction. Defined, protein-free additions (glucose, galactose, salts) may be filtered at 0.1 µm.
Each manufacturing batch is released to a specification of < 0.05 EU/mL by LAL assay (USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL). Endotoxin is controlled per batch, not per individual unit; a Certificate of Analysis is available for each lot.
Yes. A full CoA per lot covers: appearance, pH (USP <791>), osmolality (USP <785>), sterility (USP <71>), endotoxin (USP <85>), mycoplasma-retentive filtration status, particulate count (USP <788> Method 1), and raw-material traceability. Request at support@diagnocine.com.
Scientific References

Supporting literature

Key publications supporting glucose-free DMEM in metabolic flux analysis, isotope tracing, and organ-on-a-chip metabolic studies.

  1. Warburg O. On the origin of cancer cells. Science. 1956;123:309–314. doi:10.1126/science.123.3191.309
  2. Jain M, et al. Metabolite profiling identifies a key role for glycine in rapid cancer cell proliferation. Science. 2012;336:1040–1044. doi:10.1126/science.1218595
  3. Birsoy K, et al. An essential role of the mitochondrial electron transport chain in cell proliferation is to enable aspartate synthesis. Cell. 2015;162:540–551. doi:10.1016/j.cell.2015.07.016
  4. Sullivan LB, et al. Supporting aspartate biosynthesis is an essential function of respiration in proliferating cells. Cell. 2015;162:552–563. doi:10.1016/j.cell.2015.07.017
  5. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  6. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  7. Schell JC, et al. A role for the mitochondrial pyruvate carrier as a repressor of the Warburg effect and colon cancer cell growth. Mol Cell. 2014;56:400–413. doi:10.1016/j.molcel.2014.09.026
  8. Faubert B, et al. Lactate metabolism in human lung tumors. Cell. 2017;171:358–371. doi:10.1016/j.cell.2017.09.019
  9. Novak R, et al. Robotic fluidic coupling and interrogation of multiple vascularized organ chips. Nat Biomed Eng. 2020;4:407–420. doi:10.1038/s41551-019-0497-x

Related Products

Recently Viewed

Satisfaction
Quality Rating
Value Rating
Style Rating

X