Flamma® 749 Dichlorotriazine
Size of product (mg)
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Trial
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1 mg
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5 mg
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25 mg
Flamma® 749 Dichlorotriazine
Cat. No. List below
Description
Flamma® Fluors 749 Dichlorotriazine is an advanced hydroxyl-reactive near-infrared (NIR) fluorescent dye derived from cyanine structure, specifically designed for generating stable fluorescence signals in bioimaging applications. This reactive form of the dye features a dichlorotriazine group that enables unique labeling possibilities, particularly for polysaccharides and alcohols.
The dye exhibits excitation and emission maxima at 749 nm and 774 nm, respectively, positioning it firmly in the NIR region of the spectrum. These spectral characteristics are comparable to other popular NIR dyes such as Alexa 750, Cy7, IRDye 750, and DyLight 755. Flamma 749 can be efficiently excited using a 750 nm laser line or dye-pumped laser, with emission occurring in the NIR region. This spectral range is particularly advantageous for biological tissue imaging due to improved penetration and reduced autofluorescence.
The dichlorotriazine group of Flamma® Fluors 749 Dichlorotriazine reacts with hydroxyl groups through a nucleophilic substitution mechanism. Hydroxyls irreversibly displace one of the chlorines on the triazine ring, resulting in the formation of an aryl ether linkage. This reaction is particularly effective for labeling polysaccharides and other alcohols in aqueous solutions, provided that the pH is above 9 and other nucleophiles are not present.
Flamma® Fluors 749 Dichlorotriazine is particularly useful for bioimaging (generates stable fluorescence signals in various biological imaging applications), polysaccharide labeling (enables direct labeling of polysaccharides in aqueous solutions), alcohol labeling (can label alcohols on biomolecules for cellular labeling and detection), and glycoprotein analysis (useful for studying glycosylation patterns on proteins).
For optimal labeling, the following conditions should be considered:
- pH > 9 for efficient reaction with hydroxyl groups
- Aqueous solution preferred
- Absence of competing nucleophiles
1. Reduced photobleaching compared to visible light fluorophores
2. Lower background fluorescence in biological samples
3. Potential for multiplexing with other fluorophores in different spectral regions
4. Enhanced tissue penetration for in vivo imaging applications
The dichlorotriazine functionality of this dye also opens up possibilities for selective labeling of hydroxyl-containing biomolecules, studies on carbohydrate metabolism & trafficking, and development of glycan-specific probes. These properties make Flamma® Fluors 749 Dichlorotriazine a valuable tool for researchers in fields such as glycobiology, carbohydrate chemistry, and biomedical imaging, where specific labeling of hydroxyl-containing molecules and high-sensitivity detection are crucial. Its unique reactivity expands the toolkit available for bioconjugation and probe development in the NIR spectral range, particularly for studying polysaccharides and glycoproteins in complex biological systems.
- Fluorophore: Flamma® Fluors 749
- Reactive group: Dichlorotriazine
- Excitation/Emission Max.(nm): 749/774
- Spectrally similar dyes: Alexa750, DyLight755, Cy7, IRDye750
- Extinction coefficient: ≥ 200,000 cm-1M-1
- CF280: 0.035
- Appearance: Green Solid
- Molecular Weight: 900.93 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | Series | EXmax (nm) | EMmax (nm) | Spectrally similar dyes |
| PWR2112 | Flamma® 552 Dichlorotriazine | 550 | 564 | Alexa555, DyLight550, Cy3, ATTO550 |
| KWR2415 | Flamma® 581 Dichlorotriazine | 578 | 593 | Alexa594, DyLight594 |
| PWR2215 | Flamma® 648 Dichlorotriazine | 648 | 667 | Alexa647, DyLight650, Cy5 |
| PWR2515 | Flamma® 675 Dichlorotriazine | 675 | 691 | Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680 |
| PWR2301 | Flamma® 749 Dichlorotriazine | 749 | 774 | Alexa750, DyLight755, Cy7.5, IRDye750 |
| PWR2603 | Flamma® 774 Dichlorotriazine | 774 | 800 | DyLight800, Cy7.5, IRDye800, CF770 |
Background
Flamma® Fluors
BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.
- Covering the full spectral range from UV to NIR
- Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
- High quantum yields and photostability
- High purity and compatible with most of biomolecules
Flamma® Fluors Dichlorotrazine
Flamma® Fluors dichlorotriazine is for labeling hydroxyl groups. Hydroxyls irreversibly displace one of chlorine at triazine ring via SNAr reaction pathway to yield an aryl ether linkage. Dichlorotriazines are among the few reactive groups that are reported to react directly with polysaccharides and other alcohols in aqueous solution, provided that the pH is >9 and other nucleophiles are not present.
Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma® Fluors
Figure 2. Immunofluorescence imaging and in situ hybridization imaging
Figure 3. Fluorescence images of Flamma® 749 (upper) and Flamma® 774 (bottom) carboxylic acid injected mouse model
Citation & Reference
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