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Flamma® 648 Dichlorotriazine

Product#: PWR2215

$7,395.20

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5 mg
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Flamma® 648 Dichlorotriazine

Cat. No. List below

Description

Flamma® Fluors 648 Dichlorotriazine is a state-of-the-art hydroxyl-reactive far-red fluorescent dye engineered for exceptional performance in cellular labeling and detection. Derived from a cyanine structure, this innovative dye offers unparalleled stability and specificity for a wide range of biological applications, particularly in polysaccharide and alcohol labeling.

Key features:
1. Excitation/Emission maxima: 648/667 nm
2. High extinction coefficient: ≥ 250,000 cm?¹M?¹
3. Low CF280: 0.02
4. Dichlorotriazine reactive group for specific hydroxyl labeling
5. Blue solid appearance
6. Molecular weight: 874.90 g/mol
7. Soluble in DMF and DMSO

Applications:
1. Polysaccharide labeling
2. Alcohol-containing biomolecule tagging
3. Advanced cellular labeling and detection
4. Glycoprotein research
5. Carbohydrate imaging studies
6. In vitro and in vivo imaging

Advantages:
1. Unique hydroxyl reactivity in aqueous solutions (pH >9)
2. Forms stable aryl ether linkages through irreversible chlorine displacement
3. Excellent optical properties when excited using 593 or 633 nm laser lines
4. High pH (>9) labeling allows for selective targeting of hydroxyl groups
5. Minimal reactivity with other nucleophiles under proper conditions
6. Bright and stable fluorescence signals for extended imaging sessions
7. Compatibility with common fluorescence instrumentation

Specifications

Fluorophore: Flamma® Fluors 648
Reactive group: Dichlorotriazine
Excitation/Emission Max.(nm): 648/667
Spectrally similar dyes: Alexa647, DyLight650, Cy5
Extinction coefficient: ≥ 250,000 cm-1M-1
CF280: 0.02
Appearance: Blue Solid
Molecular Weight: 874.90 g/mol
Solubility: DMF, DMSO
Storage conditions: -20 ℃, protect from light

Quick link (Cat.#)	Series	EXmax (nm)	EMmax (nm)	Spectrally similar dyes
PWR2112	Flamma® 552 Dichlorotriazine	550	564	Alexa555, DyLight550, Cy3, ATTO550
KWR2415	Flamma® 581 Dichlorotriazine	578	593	Alexa594, DyLight594
PWR2215	Flamma® 648 Dichlorotriazine	648	667	Alexa647, DyLight650, Cy5
PWR2515	Flamma® 675 Dichlorotriazine	675	691	Alexa680, DyLight680, Cy5.5, IRDye680LT, CF680
PWR2301	Flamma® 749 Dichlorotriazine	749	774	Alexa750, DyLight755, Cy7.5, IRDye750
PWR2603	Flamma® 774 Dichlorotriazine	774	800	DyLight800, Cy7.5, IRDye800, CF770

Background

Flamma® Fluors

BioActs offers a broad range of Flamma® Fluors dyes equipped with variety of reactive and functional groups, which can cover the full spectral range from UV to NIR with their excellent fluorescence performance. Characteristic features of these superior dyes are strong absorption, high fluorescence quantum yield and high photostability. Flamma® dyes maintain good fluorescence activity and stability after conjugation to biomolecules and allow the detection of low-abundance biological structures with great sensitivity. The dyes are compatible with optical conditions of most of fluorescent equipment and are ideal for any applications in biological studies.

Covering the full spectral range from UV to NIR
Equipped with a variety of reactive groups: NHS and Sulfo-NHS ester, Vinylsulfone, Maleimide, Click chemistry, isothiocyanate, hydrazide and hydrophobic substances.
High quantum yields and photostability
High purity and compatible with most of biomolecules

Advantages:

Versatile Excitation: Can be efficiently excited using 593 or 633 nm laser lines, compatible with common confocal microscopy setups.
Excellent Optical Properties: Delivers bright, clear signals for high-quality imaging results.
Specific Labeling: Ideal for targeted labeling of polysaccharides and alcohol-containing biomolecules.
Stability: Forms stable aryl ether linkages for reliable, long-term imaging experiments.

Flamma^® Fluors Dichlorotrazine

Flamma^® Fluors dichlorotriazine is for labeling hydroxyl groups. Hydroxyls irreversibly displace one of chlorine at triazine ring via S_NAr reaction pathway to yield an aryl ether linkage. Dichlorotriazines are among the few reactive groups that are reported to react directly with polysaccharides and other alcohols in aqueous solution, provided that the pH is >9 and other nucleophiles are not present.

Figure 1. Absorption (upper) and emission (bottom) spectra overlap of Flamma^® Fluors

Figure 2. Immunofluorescence imaging and in situ hybridization imaging

Figure 3. Fluorescence images of Flamma^® 749 (upper) and Flamma^®774 (bottom) carboxylic acid injected mouse model

Citation & Reference

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