FAM NHS ester
FAM NHS ester
Cat. No. List below
Description
Key features:
1. Bright green fluorescence
2. Excitation/Emission maxima: 494/518 nm
3. Extinction coefficient: ≥ 64,000 cm?¹M?¹
4. CF280: 0.3
5. Molecular Weight: 473 g/mol
6. Appearance: Orange solid
7. Solubility: DMF
8. Storage: -20°C, protected from light
Applications:
1. Labeling of antibodies, peptides, proteins, and nucleotides
2. Bioimaging and fluorescence microscopy
3. Flow cytometry
4. Fluorescence in situ hybridization (FISH)
5. Real-time PCR and other nucleic acid detection methods
Advantages:
1. Excellent optical properties with 488 nm laser line excitation
2. Forms stable amide bonds with biomolecules
3. High reactivity towards amine groups
4. Good water solubility of labeled conjugates
5. Compatible with a wide range of fluorescent instrumentation
The NHS ester reactive group of FAM readily reacts with amine-modified oligonucleotides or amino groups of proteins, such as the ε-amino groups of lysine or the amine terminus of nucleotides. This reaction forms a chemically stable amide bond between the dye and the target biomolecule, ensuring long-lasting labeling for various experimental applications.
FAM NHS ester offers researchers a reliable and efficient method for fluorescent labeling in life science research, combining bright fluorescence with versatile conjugation chemistry for a wide range of biological studies.
- Fluorophore: FAM
- Reactive group: NHS ester
- Excitation/Emission Max.(nm): 494/518
- Spectrally similar dyes: Alexa488, Cy2
- Extinction coefficient: ≥ 64,000 cm-1M-1
- CF280: 0.3
- Appearance: Orange Solid
- Molecular Weight: 473 g/mol
- Solubility: DMF
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | Series | Quick link (Cat.#) | Series |
| CWS1025 | FAM NHS ester | CWSN1025 | FAM Sulfo-NHS ester |
| CWA1020 | FAM Vinylsulfone | CWM1003 | FAM Maleimide |
| CWZ1003 | FAM Azide | CWH1003 | FAM Hydrazide |
| CWK1003 | FAM Alkyne | PWK1701 | FAM PEG4-Alkyne |
| DWF1001 | FAM ADIBO | CWE1003 | FAM Amine |
| CWT1003 | FAM Thiol | CWR2003 | FAM Dichlorotriazine |
Background
Other Labeling Dyes
BioActs provides other traditionally used dyes such as 5(6)-Carboxyfluorescein (FAM) and 5(6)-Carboxytetramethylrhodamine (TAMRA) dyes for labeling of biomolecules. FAM is one of popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. In addition to relatively high absorptivity, good fluorescence quantum yield and good water solubility, FAM has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser. TAMRA fluorophore has been a widely used for preparing bioconjugates, especially fluorescent antibody and avidin derivatives. TAMRA dye is also widely utilized for oligonucleotide labeling and automated DNA sequencing applications. TAMRA is often used as FRET acceptor for FAM fluorophore.
- Other fluorescent dyes such as Cyanine, ICG, TAMRA, FAM, etc. are also available.
- All dyes are equipped with various reactive and functional groups.
- High quality and excellent performance
Table 1. FAM dye applications
Figure 1. Structure of FAM and TAMRA dyes
Citation & Reference
1. PYARE L. KHANNAA. 4',5'-Dimethoxy-6-carboxyfluorescein: a novel dipole-dipole coupled fluorescence energy transfer acceptor useful for fluorescence immunoassays. Anal Biochem 108.1 (1980): 156-61.
2. Torimura M. Fluorescence-quenching phenomenon by photoinduced electron transfer between a fluorescent dye and a nucleotide base. Anal Sci 17.1 (2001): 155-60.
3. Sylvie Soulie-Begu. In-vivo pharmacokinetic study of two fluorescein derivatives by fluorescence spectroscopy. Optical Biopsies 2627 (1995).
4. Danny van Lierop. Positively charged silver nanoparticles and their effect on Surfaceenhanced Raman scattering of dye-labelled oligonucleotides. Chem. Commun 48 (2012): 8192-8194.
5. Pete Theisen. Fluorescent Dye Phosphoramidite Labelling of Oligonucleotides. Tetrahedron Letters 33.35 (1992): 5033-5036.
