FAM Hydrazide
Size of product (mg)
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5 mg
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25 mg
FAM Hydrazide
Cat. No. List below
Description
FAM Hydrazide is a reactive form of bright green dye used to generate a stable fluorescence signal in bioimaging. The maxima of Ex/Em values are at 492/519 nm, similar to that of Alexa 488 and Cy2. FAM might be excited using 488 nm laser line and displays good optical property. Hydrazides can label aldehyde and ketone through reductive amination reaction to form an imine linkage. The main labeling target for hydrazides are free reducing sugars on biomolecules, and prior to conjugation, primary and secondary alcohols on polysaccharide and glycoprotein are usually oxidized to aldehyde and ketone. We offer FAM hydrazide for labeling of polysaccharide, glycoprotein and other biomolecules bearing aldehyde or ketone.
- Fluorophore: FAM
- Reactive group: Hydrazide
- Excitation/Emission Max.(nm): 492/519
- Spectrally similar dyes: Alexa488, Cy2
- Extinction coefficient: ≥ 59,000 cm-1M-1
- Appearance: Yellow Solid
- Molecular Weight: 392.36 g/mol
- Solubility: DMF, DMSO
- Storage conditions: -20 ℃, protect from light
| Quick link (Cat.#) | Series | Quick link (Cat.#) | Series |
| CWS1025 | FAM NHS ester | CWSN1025 | FAM Sulfo-NHS ester |
| CWA1020 | FAM Vinylsulfone | CWM1003 | FAM Maleimide |
| CWZ1003 | FAM Azide | CWH1003 | FAM Hydrazide |
| CWK1003 | FAM Alkyne | PWK1701 | FAM PEG4-Alkyne |
| DWF1001 | FAM ADIBO | CWE1003 | FAM Amine |
| CWT1003 | FAM Thiol | CWR2003 | FAM Dichlorotriazine |
Background
Other Labeling Dyes
BioActs provides other traditionally used dyes such as 5(6)-Carboxyfluorescein (FAM) and 5(6)-Carboxytetramethylrhodamine (TAMRA) dyes for labeling of biomolecules. FAM is one of popular green fluorescent reagents used for labeling peptides, proteins and nucleotides. In addition to relatively high absorptivity, good fluorescence quantum yield and good water solubility, FAM has an excitation maximum that closely matches the 488 nm spectral line of the argon-ion laser. TAMRA fluorophore has been a widely used for preparing bioconjugates, especially fluorescent antibody and avidin derivatives. TAMRA dye is also widely utilized for oligonucleotide labeling and automated DNA sequencing applications. TAMRA is often used as FRET acceptor for FAM fluorophore.
- Other fluorescent dyes such as Cyanine, ICG, TAMRA, FAM, etc. are also available.
- All dyes are equipped with various reactive and functional groups.
- High quality and excellent performance
Table 1. FAM dye applications
Figure 1. Structure of FAM and TAMRA dyes
Citation & Reference
1. PYARE L. KHANNAA. 4',5'-Dimethoxy-6-carboxyfluorescein: a novel dipole-dipole coupled fluorescence energy transfer acceptor useful for fluorescence immunoassays. Anal Biochem 108.1 (1980): 156-61.
2. Torimura M. Fluorescence-quenching phenomenon by photoinduced electron transfer between a fluorescent dye and a nucleotide base. Anal Sci 17.1 (2001): 155-60.
3. Sylvie Soulie-Begu. In-vivo pharmacokinetic study of two fluorescein derivatives by fluorescence spectroscopy. Optical Biopsies 2627 (1995).
4. Danny van Lierop. Positively charged silver nanoparticles and their effect on Surfaceenhanced Raman scattering of dye-labelled oligonucleotides. Chem. Commun 48 (2012): 8192-8194.
5. Pete Theisen. Fluorescent Dye Phosphoramidite Labelling of Oligonucleotides. Tetrahedron Letters 33.35 (1992): 5033-5036.
