Fast Green FCF Staining Solution
Cat No.: DCP-FGFCF1X
Size: 500 mL
Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Fast Green FCF Staining Solution is a high-purity histological and biochemical staining reagent optimized for visualizing collagen, cytoplasmic structures, and proteins in electrophoresis and tissue sections. Known for its brilliant and stable coloration, it is widely used in histological investigations, electrophoresis techniques, and quantitative staining of proteins. This Fast Green FCF Staining Solution is ideal for researchers seeking reliable staining results across histological and biochemical applications while minimizing preparation time.
Applications:
1. Histological Staining
- Collagen Visualization: Binds selectively to collagen fibers, producing intense green coloration in Masson-Goldner Trichrome and Paraldehyde Fuchsin protocols.
- Cytoplasmic & Muscle Staining: Highlights cytoplasmic details and muscle fibers in formalin-fixed, paraffin-embedded tissues.
- Counterstain: Compatible with hematoxylin (Weigert’s iron) and fuchsin acid in multicolor staining workflows.
2. Protein Analysis
- Electrophoresis: Linear quantitative staining of histones (alkaline pH) and total proteins in IEF and SDS-PAGE (0.1–20 µg/band).
- Fluorescence Detection: Near-infrared emission (λmax 624 nm) enables sensitive imaging without organic solvent interference.
Advantages:
- Brilliant Coloration: Provides intense green staining that is resistant to fading.
- Wide Application Range: Suitable for histology, electrophoresis, and quantitative protein analysis.
- High Stability: The solution remains stable for up to 6 months when stored properly.
Composition:
| Ingredients |
Concentration |
| Fast Green FCF powder |
0.1% |
| Acetic Acid |
1% |
Quality Control:
- Appearance: Deep Green solution
- Sterility: Filtered 0.1 micron Twice and 0.04 micron once.
- DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
- RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
- Store at room temperature away from bright light.
- Shelf life is 6 months.
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
