Methyl Green Solution
Cat No.: DCP-MG0.1X
Size: 500 mL
Sterile, Ultrapure, Filtered 0.1-micron membrane TWICE and 0.04-micron membrane ONCE.
Description:
Methyl Green 0.1% w/v aqueous solution is a cationic triphenylmethane dye widely employed in biological staining and molecular biology applications. This ready-to-use formulation is optimized for nuclear counterstaining, DNA visualization, and compatibility with advanced detection systems. With a clear blue-green appearance and pH-dependent color transition (yellow at pH 0.1 to blue at pH 2.3), it serves as a critical tool for researchers requiring precise nucleic acid differentiation and cellular imaging.
Key Features:
- Ready-to-use nuclear counterstain optimized for histology, cytology, and molecular biology applications.
- Pre-purified via chloroform extraction to remove crystal violet contaminants, ensuring consistent staining quality.
- Exhibits far-red fluorescence when bound to DNA (excitation: 633 nm; emission: 677 nm), enabling high-resolution confocal imaging.
- Non-intercalating DNA binding via electrostatic interactions with AT-rich regions, preserving nucleic acid integrity.
- Compatible with aqueous and non-aqueous mounting media, including resin-based formulations.
Applications:
1. Nuclear Staining in Histology/Cytology:
- Provides crisp light-green nuclear contrast in formalin-fixed, paraffin-embedded (FFPE) tissues and frozen sections.
- Pairs with chromogenic substrates (e.g., DAB) in immunohistochemistry (IHC) and in situ hybridization (ISH).
2. DNA/RNA Differentiation:
- Used in Methyl Green-Pyronin staining to distinguish DNA (green) from RNA (red) in cellular samples.
3. Fluorescent Imaging:
- Serves as a photostable, far-red fluorescent marker for live-cell nuclei imaging and thick tissue sections.
4. Electrophoresis:
- Functions as a tracking dye in acidic buffer systems for DNA gel electrophoresis.
5. Educational & Diagnostic Use:
- Ideal for teaching laboratories and clinical settings requiring reliable nuclear visualization.
Composition:
| Ingredients |
Concentration |
| Methyl Green dye |
0.1% |
Usage Protocol:
Staining Procedure:
- Apply directly to slides for 1–5 minutes at 60°C (heated Coplin jar) or room temperature.
- Rinse with deionized water and dehydrate through ethanol/xylene series.
Fluorescent Labeling:
Quality Control:
- Appearance: Blue-green liquid
- Sterility: Filtered 0.1 micron Twice and 0.04 micron once.
- DNase activity: None was detected after incubation of plasmid DNA, and this product was used for 18 hours at room temperature.
- RNase activity: No RNase activity was detected after incubation of ribosomal RNA and this product for 18 hr at room temperature.
Storage and Shelf Life:
- Store at room temperature away from bright light.
- Shelf life is 24 months unopened.
- Use before expiry date given on the product label.
CUSTOMIZATION: Please INQUIRE if other concentrations, additions of chemicals/compounds/proteins/supplements, different pH, and modifications are needed.
All DiagnoCine Precision Sterile buffers are filtered-sterilized with a 0.1-micron filtration two times and 0.04-micron filtration one time. Thus, mycoplasma contamination is prevented. The smallest size mycoplasma type can be about 0.2 microns.
* This product is manufactured under ISO 13485-certified and CE-approved facilities (Suppliers of DiagnoCine Precision). All final packaging, quality assurance, and testing are done at the DiagnoCine R&D and Quality Testing Center. All specific customization requests and assembly were accomplished at DiagnoCine Precision in Totowa, New Jersey, USA.
