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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Pyruvate: 1X Liquid

Product#: DCP-DMEMH-GP1X
$44.00
DCP-DMEMH-GP1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Pyruvate: 1X Liquid

Contains Sodium Bicarbonate Contains Phenol Red Contains HEPES (25mM) Contains Calcium Contains Magnesium Without Glucose Without Sodium Pyruvate

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid DMEM base engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Formulated with 25 mM HEPES and sodium bicarbonate, without glucose or sodium pyruvate, for precise, user-defined metabolic control. Manufactured under an ISO 13485:2016 quality management system with per-lot release testing.

  • Quadruple-stage filtration train (0.1 µm ×2 + 0.04 µm ×2) reaching a 0.04 µm final cut-off — Microfluidics Suitable
  • Endotoxin release specification: < 0.05 EU/mL (USP <85> BET, per batch)
  • Formulated with 25 mM HEPES and sodium bicarbonate; glucose-free and sodium-pyruvate-free for user-defined metabolic control
  • Contains added calcium and magnesium salts, plus a phenol red pH indicator
  • 4× BME vitamin concentrations (see Vitamins tab) — amino acids are not included in this base formulation
  • Manufactured under an ISO 13485:2016 quality management system; final QC at Diagnocine, Totowa, NJ
  • Custom pH, glucose, HEPES, salts & nutrients available on request — support@diagnocine.com
Incomplete basal medium. This formulation contains no glucose and no sodium pyruvate. A carbon source, serum or defined supplements must be added before use. See the FAQ for supplementation guidance.
DCP-DMEMH-GP1X · Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Pyruvate: 1X Liquid
  • GlucoseNot added
  • L-GlutamineNot added
  • Sodium PyruvateNot added
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)285 - 325 mOsm/kg
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1µm ×2 + 0.04µm ×2 (Quadruple-stage)
  • Storage2-8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack (2–8°C)
Available sizes: 500 mL, 1000 mL
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and invalidating metabolic assays. FluxMPS™ closes that gap.

filter_alt

Microchannel-safe purity

0.04 µm final filtration reduces sub-micron particulates and microaggregates that can obstruct narrow microfluidic channels.

target

Total metabolic control

Precisely defined carbon sources enable Warburg-effect studies, glycolysis inhibition, and ¹³C metabolic tracing without background noise.

water_drop

Ultrapure-grade water

Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) with low total organic carbon and trace-metal control for consistent, reproducible formulation.

visibility

Low background for imaging

Ultra-low particulate baseline for confocal live-cell imaging, fluorescent biosensors, and automated high-content analysis on chip.

science

Rich, stable nutrient profile

4× BME vitamin concentrations with micro-batch precision — amino acids are not included in this base formulation and should be supplemented per your protocol.

tune

Customization on demand

pH, glucose, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

A validated four-stage sequential filtration train reaching a final pore size of 0.04 µm — addressing particulates and mycoplasma-sized contaminants that pass through standard 0.22 µm filtration.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm final filter.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter.

  3. 3

    0.1 µm Prefiltration II

    Second dedicated 0.1 µm mycoplasma-retentive prefilter, protecting the second 0.04 µm final filter cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill & finish in a controlled ISO Class 5 environment.

Performance vs. conventional media

By USP <788> Method 1 (light obscuration), FluxMPS™ is designed to deliver substantially fewer particles ≥10 µm than standard 0.22 µm filtered media — a meaningful difference for long-duration microfluidic perfusion.

5×
Fewer particles ≥10 µm vs 0.22 µm media
0.04
µm Final filter — sub-mycoplasma polishing
Sterility & Mycoplasma: 14-day USP <71> sterility tested per lot. Mycoplasma risk is controlled by 0.1 µm mycoplasma-retentive filtration (not tested per lot); typical mycoplasma diameter is 0.2–0.3 µm.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Pyruvate: 1X Liquid (DCP-DMEMH-GP1X) ? Quadruple-stage filtration system (0.1 μm x2 + 0.04 μm x2) for organ-on-a-chip and microfluidic MPS cell culture | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration: 0.1µm Prefiltration I → 0.04µm Final filtration I → 0.1µm Prefiltration II → 0.04µm Final filtration II (Polish) → aseptic fill & finish.
© Diagnocine® — DCP-DMEMH-GP1X
Applications

Optimized for next-generation cell biology platforms

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Pyruvate: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated perfusion and robotic liquid-handling platforms, Diagnocine also offers an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation — a distinct product line from the Microfluidics Suitable (0.04 µm) medium described on this page.

  • Total Particulate Exclusion — 0.01 µm final cut-off for the most sensitive automated systems
  • Valve & Sensor Protection — minimizes fouling in long-running robotic fluidics
  • Extended Perfusion Stability — supports multi-week unattended bioreactor runs

Inquiry Required: the 0.01 µm MPS Grade variant is available on request. Contact support@diagnocine.com.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-low particulate, filtration-controlled media for perfusion in organ chips, tissue chips (ToC), and body-on-a-chip (BoC) devices.

OoCToCBoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Glucose-free formulation for Warburg-effect studies, aerobic glycolysis, and cancer metabolomics with user-defined carbon sources.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Ultra-clean, low-endotoxin baseline (following carbon-source supplementation) minimizes non-specific signals in iPSC differentiation and functional organoid readouts.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Particle-controlled perfusion media for TEER measurement, endothelial monolayer integrity, and primary cell culture.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Chemically defined, glucose-free formulation for ¹³C metabolic tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

¹³C tracingNMR
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate baseline supports confocal microscopy, fluorescent biosensors, and automated imaging on chip.

ConfocalBiosensorsTEER
Technical Specifications

Full technical specification

Every lot of FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Pyruvate: 1X Liquid is released against comprehensive multi-parameter QC specifications.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Sodium Bicarbonate, Phenol Red, HEPES (25mM), Calcium, Magnesium / [-] Glucose, Sodium Pyruvate
Appearance Red to pink-red, clear solution (phenol red pH indicator present)
pH USP <791> 7.4
Osmolality USP <785> 285 - 325 mOsm/kg H&sub2;O
Glucose Not added
L-Glutamine Not added (not listed in composition)
Sodium Pyruvate Not added
Phenol Red 15.9 mg/L
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> < 0.05 EU/mL (batch release; see § Manufacturing & Compliance)
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing ISO 13485:2016 ISO
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage 2-8°C, protect from light
Freeze–thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping Cold pack (2–8°C)
CO&sub2; requirement ~10% CO&sub2; (derived from 44 mM sodium bicarbonate to maintain pH 7.4; supplemental 25 mM HEPES buffering also present)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Pharmaceutical/research grade CoA
Manufacturing QMS ISO 13485:2016 ISO
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory 21 CFR Part 820 (QMSR) aligned
Production Micro-batch; Totowa, NJ, USA
Intended use RUO only
Formulation

Full composition (mg/L)

Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 17 components across 3 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).

Component CAS Number mg/L
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 3700.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
Component CAS Number mg/L
No amino acids listed for this formulation
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
OTHERS
Phenol red sodium salt 34487-61-1 15.900
HEPES 7365-45-9 5958.000
i-Inositol 87-89-8 7.200
Custom formulations: pH, glucose, HEPES, salts, and individual nutrient levels adjustable on request. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Every batch is subjected to multi-parameter lot-release testing before distribution.

verified

ISO 13485:2016 QMS

Manufactured by ISO 13485-certified suppliers. Final packaging, QA and testing at Diagnocine R&D Center; customization at Diagnocine Precision, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm resistivity with low total organic carbon and trace-metal control for consistent, reproducible formulation.

biotech

ISO Class 5 Fill & Finish

Validated ISO Class 5 laminar-flow workstation with real-time particle monitoring, preserving filtration gains through final fill.

assignment

Micro-Batch Precision

Small-batch production for tight lot-to-lot consistency — important for reproducible long-duration MPS perfusion experiments.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request.

Endotoxin — USP <85> BET

LAL assay. Release specification: < 0.05 EU/mL per manufacturing batch.

Particulate — USP <788> Method 1

Light-obscuration particle count testing performed per lot; released within specification.

Osmolality — USP <785>

Freezing-point depression osmometry. Release range: 285 - 325 mOsm/kg H&sub2;O.

Certificate of Analysis (CoA)

Full CoA per lot. Request at support@diagnocine.com with lot number.

CoA Request: Available at no charge for any production lot of FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Pyruvate: 1X Liquid (DCP-DMEMH-GP1X). Email support@diagnocine.com.
Product Comparison

How DCP-DMEMH-GP1X compares

Key differences in grade, filtration, mycoplasma control, and QC depth versus conventional media for microfluidic and MPS applications.

Parameter DCP-DMEMH-GP1X (FluxMPS™) Standard DMEM (0.22 µm) Competitor DMEM (0.22 µm)
Grade Microfluidics Suitable (0.04 µm final cut-off) Not applicable Not applicable
Formulation definition [+] Sodium Bicarbonate, Phenol Red, HEPES (25mM), Calcium, Magnesium / [-] Glucose, Sodium Pyruvate Standard Standard
Final filtration pore size 0.04 µm (40 nm) 0.22 µm 0.22 µm
Filtration stages 4 (Quadruple) 1 1
Mycoplasma-retentive filtration (0.1 µm) check_circle cancel cancel
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> Method 1 particulate testing check_circle Tested per lot cancel cancel
Water quality Ultrapure Type 1, 18.2 MΩ·cm Unspecified Unspecified
Manufacturing QMS ISO 13485:2016 Varies Varies
Microfluidic channel compatibility check_circle cancel cancel
Custom formulation check_circle On request cancel cancel

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, Sodium Pyruvate: 1X Liquid (DCP-DMEMH-GP1X).

DCP-DMEMH-GP1X is Microfluidics Suitable and engineered for organ-on-a-chip, microfluidic, and MPS applications. The 0.04 µm final filtration reduces sub-micron particulates that cause microchannel clogging in standard 0.22 µm media, and the 0.1 µm stages provide mycoplasma-retentive filtration.
Four sequential stages (0.1 µm ×2 + 0.04 µm ×2) address mycoplasma-sized organisms (0.2–0.3 µm diameter), sub-visible particulates, and bioburden largely missed by conventional 0.22 µm filters, tested by USP <788> Method 1 (light obscuration).
DCP-DMEMH-GP1X is formulated without glucose or sodium pyruvate to enable precise metabolic control — including Warburg-effect studies, glycolysis inhibition, and ¹³C carbon-source tracing. Add D-glucose at your desired concentration (typically 1-4.5 g/L) or substitute galactose or pyruvate as your assay requires. Contact support@diagnocine.com for pre-supplemented variants.
This medium contains 44 mM sodium bicarbonate (3700 mg/L). To hold pH 7.4 with that bicarbonate concentration requires approximately 10% CO&sub2;, not the 5% CO&sub2; typical of lower-bicarbonate formulations. The 25 mM HEPES present provides additional buffering capacity for brief periods outside the incubator.
Yes. DCP-DMEMH-GP1X is a basal medium compatible with FBS, human serum, growth factors, and antibiotics. Pre-filter serum-containing additions through a 0.2 µm low-protein-binding PES or PVDF syringe filter (defined, protein-free additions may use 0.1 µm) before combining with the medium.
Endotoxin is controlled per manufacturing batch, not per unit. Every batch is tested by LAL assay (USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before distribution. Lot-specific results are available on the CoA — request at support@diagnocine.com.
Yes — a full CoA per lot includes: lot number, expiry, appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85>), sterility (USP <71>), and particulate testing (USP <788>). Email support@diagnocine.com.
Scientific References

Supporting literature

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
  4. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
  5. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
  6. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
  7. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
  8. Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x

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