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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate: 1X Liquid

Product#: DCP-DMEMH-GQP1X
$44.00
DCP-DMEMH-GQP1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate: 1X Liquid

Contains Sodium Bicarbonate Contains Phenol Red Contains 25mM HEPES Contains Calcium Contains Magnesium Without Glucose Without L-Glutamine Without Sodium Pyruvate

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 in an ISO Class 5 fill environment using Ultrapure Type 1 water (18.2 MΩ·cm). A quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reaches a 0.04 µm final cut-off, five times finer than the 0.22 µm membranes used for conventional sterile filtration.

  • Quadruple-stage filtration to a 0.04 µm final cut-off (0.1 µm ×2 + 0.04 µm ×2) — validated mycoplasma-retentive polishing for microfluidic channels
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85> BET), tested per manufacturing batch
  • Formulated without glucose, L-glutamine, or sodium pyruvate — define your own carbon source and nitrogen supplementation
  • Buffered with 3700 mg/L sodium bicarbonate plus 25 mM HEPES for flexible CO2-incubator or bench-top use
  • 4× BME amino acid and vitamin concentrations for extended microphysiological system (MPS) perfusion
  • Manufactured under an ISO 13485:2016 quality management system; aseptic fill & finish at Diagnocine, Totowa, NJ
  • Custom pH, glucose, HEPES, salts & nutrients — support@diagnocine.com
Incomplete basal medium. This formulation contains no glucose and no sodium pyruvate. A carbon source, serum or defined supplements must be added before use. See the FAQ for supplementation guidance.
DCP-DMEMH-GQP1X · Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate: 1X Liquid
  • GlucoseNot added
  • L-GlutamineNot added
  • Sodium PyruvateNot added
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)310 - 350 mOsm/kg
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1µm ×2 + 0.04µm ×2
  • Storage2-8°C, away from bright light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack (2–8°C)
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and invalidating metabolic assays. FluxMPS™ closes that gap.

filter_alt

Microchannel-safe purity

Quadruple-stage filtration reaches a 0.04 µm final cut-off, with particulate levels controlled per USP <788> Method 1 (light obscuration), supporting reliable flow in narrow microfluidic channels.

target

Total metabolic control

Precisely defined carbon sources enable Warburg-effect studies, glycolysis inhibition, and 13C metabolic tracing without background noise.

water_drop

Ultrapure-grade water

Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) — supporting low trace-metal and organic-carbon (TOC) background for sensitive assay readouts.

visibility

Low background for imaging

Ultra-low particulate baseline supports confocal live-cell imaging, fluorescent biosensors, and automated high-content analysis on chip.

science

Rich, stable nutrient profile

4× BME amino acid and vitamin concentrations with micro-batch precision — consistent lot-to-lot composition for long-duration MPS perfusion.

tune

Customization on demand

pH, glucose, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

A validated four-stage sequential filtration train reaching a final pore size of 0.04 µm — engineered to reduce sub-micron particulates and microaggregates beyond what 0.22 µm filtration captures.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris, and protein aggregates. Protects the first 0.04 µm cartridge and preserves microchannel chip geometries.

  2. 2

    0.04 µm Final filtration I

    Retains fine particulates and mycoplasma-range bioburden (0.2–0.3 µm) — entirely absent from standard 0.22 µm media.

  3. 3

    0.1 µm Prefiltration II

    A second, dedicated prefilter protects the second 0.04 µm cartridge, giving the train full redundancy.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill in a validated ISO Class 5 (Class 100) laminar-flow workstation.

Performance vs. conventional media

The quadruple-stage train (0.1 µm ×2 + 0.04 µm ×2) reduces particulate and bioburden risk beyond standard 0.22 µm-filtered media, supporting reliable long-duration perfusion in microfluidic and organ-on-a-chip systems.

4
Sequential filtration passes (0.1 µm ×2 + 0.04 µm ×2)
0.04
µm final filter — sub-mycoplasma-retentive polish
Sterility & Mycoplasma: 14-day USP <71> sterility tested per batch. The 0.1 µm and 0.04 µm stages provide mycoplasma-retentive filtration (mycoplasma diameter 0.2–0.3 µm); this is a filtration control, not a per-lot mycoplasma assay.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate: 1X Liquid (DCP-DMEMH-GQP1X) - Quadruple-stage filtration system (0.1 um x2 + 0.04 um x2) for organ-on-a-chip and microfluidic MPS cell culture | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration: 0.1µm Prefiltration I → 0.04µm Final filtration I → 0.1µm Prefiltration II → 0.04µm Final filtration II (Polish) → ISO Class 5 aseptic fill.
© Diagnocine® — DCP-DMEMH-GQP1X
Formulation

Full Composition (mg/L)

Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 31 components across 4 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).

INORGANIC SALTS

Component CAS Number mg/L
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 3700.000
Sodium chloride 7647-14-5 6400.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000

AMINO ACIDS

Component CAS Number mg/L
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Histidine hydrochloride monohydrate 5934-29-2 42.000
L-Isoleucine 73-32-5 105.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 146.000
L-Methionine 63-68-3 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-45-6 109.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
i-Inositol 87-89-8 7.200
OTHERS
Phenol red sodium salt 34487-61-1 15.900
HEPES 7365-45-9 5958.000
Custom formulations: pH, glucose, HEPES, salts, and individual nutrient levels adjustable on request. Contact support@diagnocine.com.
Technical Specifications

Full technical specification

Every lot of FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate: 1X Liquid is released against comprehensive multi-parameter QC specifications. Available pack sizes: 500 mL, 1000 mL.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Sodium Bicarbonate, Phenol Red, 25mM HEPES, Calcium, Magnesium / [-] Glucose, L-Glutamine, Sodium Pyruvate
Appearance Clear, red-hued solution (phenol red present)
pH USP <791> 7.4
Osmolality USP <785> 310 - 350 mOsm/kg H2O
Glucose Not added
L-Glutamine Not added
Sodium Pyruvate Not added
Phenol Red 15.9 mg/L
Sterility, Purity & Safety
Parameter Specification
Endotoxin USP <85> < 0.05 EU/mL (batch release)
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Particulate ≥10 µm <788> Method 1 Compliant
Particulate ≥25 µm <788> Method 1 Compliant
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing ISO 13485:2016 ISO
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage 2-8°C, away from bright light
Freeze–thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping Cold pack (2–8°C)
CO2 requirement ≈10% CO2 recommended (bicarbonate-buffered; 25 mM HEPES supplemental buffering)
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Pharmaceutical/research grade CoA
Manufacturing QMS ISO 13485:2016 ISO
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory alignment 21 CFR Part 820 (QMSR) aligned
Production method Micro-batch; Totowa, NJ, USA
Intended use RUO only
Applications

Optimized for next-generation cell biology platforms

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated perfusion bioreactors and robotic culture platforms, Diagnocine offers an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation — a distinct six-stage process beyond the Microfluidics Suitable 0.04 µm line described on this page.

  • Total particulate exclusion for long-duration unattended runs
  • Valve and sensor protection in closed-loop perfusion systems
  • Extended perfusion stability for multi-week automated protocols

Inquiry Required: the 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com to request it.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-low particulate, mycoplasma-retentive-filtered media for perfusion in organ chips, tissue chips (ToC), and body-on-a-chip (BoC) devices.

OoCToCBoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Glucose-free, user-defined carbon source formulation for Warburg-effect studies, aerobic glycolysis, and cancer metabolomics.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Ultra-clean, low-endotoxin baseline minimizes non-specific signals in iPSC differentiation and functional organoid readouts.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Particle-controlled perfusion media for TEER measurement, endothelial monolayer integrity, and primary cell culture.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Chemically defined, glucose-free base for 13C metabolic tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

13C tracingNMR
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate baseline medium for confocal microscopy, fluorescent biosensors, and automated imaging on chip.

ConfocalBiosensorsTEER
Quality Assurance

Manufacturing & compliance

Every batch subjected to multi-parameter lot-release testing before distribution.

verified

ISO 13485:2016 QMS

Manufactured by ISO 13485-certified suppliers. Final packaging, QA and testing at DiagnoCine R&D Center; customization at DiagnoCine Precision, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm, low trace-metal and organic-carbon (TOC) background. Supports sensitive biosensor and imaging assays.

biotech

ISO Class 5 Fill & Finish

Validated ISO Class 5 laminar-flow workstation with real-time particle monitoring. Preserves filtration gains in the final container.

assignment

Micro-Batch Precision

Small-batch production with tight lot-to-lot consistency — critical for reproducible long-duration MPS experiments.

Endotoxin — USP <85> BET

LAL assay. Release specification: < 0.05 EU/mL. Assay sensitivity: 0.005 EU/mL.

Particulate — USP <788> Method 1

Light obscuration particle count testing per USP <788> Method 1; validated for microfluidic applications.

Osmolality — USP <785>

Freezing-point depression osmometry. Release range: 310 - 350 mOsm/kg H2O.

Certificate of Analysis (CoA)

Full CoA per lot. Request at support@diagnocine.com with lot number.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request. Email support@diagnocine.com.
Product Comparison

How DCP-DMEMH-GQP1X compares

Critical advantages in filtration, mycoplasma barrier, water quality, and QC depth that conventional media cannot match for MPS and microfluidic applications.

Parameter DCP-DMEMH-GQP1X (FluxMPS™) Standard DMEM (0.22 µm) Competitor DMEM (0.22 µm)
Grade Microfluidics Suitable Standard grade Standard grade
Formulation definition [+] Sodium Bicarbonate, Phenol Red, 25mM HEPES, Calcium, Magnesium / [-] Glucose, L-Glutamine, Sodium Pyruvate Standard Standard
Final filtration pore size 0.04 µm (40 nm) 0.22 µm 0.22 µm
Filtration stages 4 (Quadruple: 0.1→0.04→0.1→0.04) 1 1
Mycoplasma-retentive filtration check_circle cancel cancel
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance check_circle Method 1 cancel cancel
Water quality Ultrapure Type 1, 18.2 MΩ·cm Unspecified Unspecified
Manufacturing QMS ISO 13485:2016 Varies Varies
Microfluidic channel compatibility check_circle cancel cancel
Custom formulation check_circle On request cancel cancel

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose, L-Glutamine, Sodium Pyruvate: 1X Liquid (DCP-DMEMH-GQP1X).

Yes. DCP-DMEMH-GQP1X is engineered for organ-on-a-chip, microfluidic, and MPS applications. The 0.04 µm final filtration reduces mycoplasma-range bioburden and sub-micron particulates that cause microchannel clogging in standard 0.22 µm media.
Four sequential stages (0.1 µm ×2 + 0.04 µm ×2) reduce mycoplasma-range bioburden (0.2–0.3 µm diameter) and sub-visible particulates that pass through conventional 0.22 µm filters, validated per USP <788> Method 1.
This ultra-pure, quadruple-filtered base is left free of glucose, L-glutamine, and sodium pyruvate so you can define the carbon and nitrogen sources precisely — for example, glucose for standard metabolism, galactose to force oxidative phosphorylation, or L-glutamine/GlutaMAX for nitrogen supply. Add each component fresh before use; see the supplementation FAQ below for filtration guidance. Custom pre-formulated variants are available at support@diagnocine.com.
This formulation is buffered with 3700 mg/L sodium bicarbonate plus 25 mM HEPES. Maintaining pH 7.4 with this bicarbonate level requires approximately 10% CO2 (rather than the 5% used for lower-bicarbonate formulations); the added HEPES provides extra buffering stability for bench-top handling outside the incubator. Confirm CO2 setpoint against your own pH readings.
Yes. This basal medium is compatible with FBS, human serum, GlutaMAX, and growth factors such as EGF, FGF, and VEGF. Pre-filter serum and protein-containing additions through a 0.2 µm low-protein-binding PES or PVDF filter; use 0.1 µm only for defined, protein-free additions. Do not use 0.04 µm filters for supplements — they retain serum proteins and lipoproteins and clog immediately.
Endotoxin is controlled per manufacturing batch to a release specification of < 0.05 EU/mL, tested by LAL assay per USP <85> (Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL). The lot-specific value is reported on the Certificate of Analysis — request at support@diagnocine.com.
Yes — a full CoA per lot includes: lot number, expiry, appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85>), sterility (USP <71>), mycoplasma-retentive filtration statement, particulate count (USP <788> Method 1), and cultural response. Email support@diagnocine.com.
Scientific References

Supporting literature

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
  4. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
  5. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
  6. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
  7. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
  8. Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x

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