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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose w/o L-Glutamine, Sodium Bicarbonate: 2X Liquid

Product#: DCP-DMEM-QB2X
$85.25
DCP-DMEMH-QB2X
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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose w/o L-Glutamine, Sodium Bicarbonate: 2X Liquid

Double-strength (2X) DMEM High Glucose — 9.0 g/L glucose in the concentrate, 4.5 g/L at 1X — for soft-agar, collagen-gel, hydrogel and other 3D or semisolid cultures, ultra-filtered through a quadruple-stage 0.1 µm / 0.04 µm filtration system. With a bicarbonate-free base for user-defined buffering, it is approximately 5× cleaner than conventional 0.22 µm-filtered media by particulate count.

  • 2X concentrate: mix with an equal volume of a compatible solution or matrix for a final 1X medium
  • Quadruple-stage filtration: 0.1 µm membrane filtered twice, 0.04 µm membrane filtered twice
  • 0.04 µm nano-filtration final polish acts as a mycoplasma barrier
  • Endotoxin < 0.05 EU/mL (USP <85> BET)
  • Formulation: [+] 9.0 g/L Glucose, [+] Sodium Pyruvate, [+] Phenol Red; [−] L-Glutamine, [−] Sodium Bicarbonate, [−] HEPES
  • Ultrapure Type 1 water (18.2 MΩ·cm); ISO Class 5 (Class 100) aseptic fill in Totowa, NJ
  • Custom formulations available — pH, glucose, HEPES, salts, and nutrient composition on request
DCP-DMEM-QB2X|UNSPSC 41116155Cell culture media
Catalog No. DCP-DMEM-QB2X · Size: 500 mL
Dulbecco's Modified Eagle Medium (DMEM), High Glucose w/o L-Glutamine, Sodium Bicarbonate: 2X Liquid
  • D-Glucose9,000 mg/L
  • L-GlutamineNot included
  • Sodium Pyruvate220 mg/L
  • BufferNone (user-defined)
  • Phenol Red31.8 mg/L
  • AppearanceRed to Orange
  • pH7.4 (± 0.04)
  • Endotoxin< 0.05 EU/mL
  • Filtration0.1 µm ×2 + 0.04 µm ×2
  • Format2X liquid, 500 mL
ISO 13485:2016USP <85> <785> <788>RUO
Why FluxMPS™

Engineered where standard media fails

Conventional DMEM is sterile-filtered once at 0.22 µm. That pore size can pass mycoplasma and leaves subvisible particulates that settle in microchannels, foul membranes and hydrogels, and add noise to optical readouts.[11,12] FluxMPS™ DMEM keeps Dulbecco's formulation and removes that failure mode.

filter_alt

Microchannel-safe purity

A 0.04 µm final filter and USP <788> particulate control keep subvisible debris out of chips, tubing, membranes and matrices.

target

Total metabolic control

Defined 9,000 mg/L D-glucose, no L-glutamine and 220 mg/L sodium pyruvate in the 2X (half that at 1X).

water_drop

Ultrapure-grade water

Made with Ultrapure Type 1 water (18.2 MΩ·cm) and released at < 0.05 EU/mL endotoxin by USP <85>.

visibility

Low background for imaging

Particle-depleted medium reduces scatter and debris artifacts in colony counting, confocal imaging and on-chip sensing.

science

Matrix-ready 2X strength

31 components at double strength, so agar, collagen, hydrogels or treatment solutions can be added 1:1 without diluting the medium below 1X.

tune

Customization on demand

pH, glucose, HEPES, salts, and nutrient composition on request — contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

Every lot of DCP-DMEM-QB2X is membrane filtered at 0.1 µm twice and 0.04 µm twice — a ready-to-dilute 2X DMEM polished to a 0.04 µm final pore size, a purity level single-pass 0.22 µm media cannot reach.

  1. 01

    0.1 µmPre-filtration I

    Removes large particulates and aggregates and extends the life of the downstream filters.

  2. 02

    0.04 µmPre-filtration II

    Retains fine particulates, bacteria and mycoplasma.

  3. 03

    0.1 µmSterile-filtration I

    Second-pass redundancy that catches anything shed or bypassed upstream.

  4. 04

    0.04 µmSterile-filtration II — Final Polish

    Final nano-polish immediately before ISO Class 5 aseptic fill.

Performance vs. conventional media

By particulate count, FluxMPS™ DMEM is approximately 5× cleaner than conventional 0.22 µm-filtered media, lowering clog risk in narrow channels and long perfusion runs.

5×
cleaner by particulate count vs. 0.22 µm media
0.04
µm final filtration pore size
Sterility & mycoplasma assurance: no bacterial or fungal growth is observed after 14 days of incubation, as per USP specification (USP <71>), and the 0.04 µm stages provide USP <63>-equivalent mycoplasma assurance.[11]
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), High Glucose w/o L-Glutamine, Sodium Bicarbonate: 2X Liquid (DCP-DMEM-QB2X) Quadruple-stage filtration system diagram: 0.1 μm pre-filtration, 0.04 μm pre-filtration, 0.1 μm sterile filtration and 0.04 μm final polish for MPS-grade DMEM High Glucose cell culture media used in soft-agar, 3D, organ-on-a-chip and microfluidic applications, Diagnocine
Figure 1. Quadruple-stage filtration: 0.1 µm pre-filtration, 0.04 µm pre-filtration, 0.1 µm sterile filtration and 0.04 µm final polish before ISO Class 5 fill.
© Diagnocine® — DCP-DMEM-QB2X
Applications

Where FluxMPS™ DMEM fits

DMEM High Glucose is a widely used basal medium containing 4.5 g/L glucose at its 1X working concentration.[1,2] It supports many primary and established mammalian cell types, including fibroblasts, neurons, glial cells, endothelial cells, smooth-muscle cells, HEK 293, HeLa, COS-7, and other normal or transformed cell lines.[3] It is commonly used in cell-growth, transfection, recombinant-protein, tissue-engineering, drug-discovery, and cell-signaling research.

DMEM High Glucose 2X is a double-strength formulation containing 9.0 g/L glucose in the concentrate and providing 4.5 g/L glucose after dilution to 1X. It is designed to be mixed with an equal volume of a compatible solution or matrix while maintaining the intended final 1X concentration of glucose, amino acids, vitamins, and salts.

Researchers use the 2X formulation to prepare customized media and cultures containing large volumes of agar, agarose, methylcellulose, collagen, hydrogels, supplements, treatment solutions, or other aqueous components. It is particularly useful for soft-agar colony assays, collagen-gel contraction studies, semisolid cultures, 3D cell culture, stem-cell research, tissue engineering, and other workflows in which adding a matrix or solution would dilute conventional 1X DMEM.[4,5]

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated bioreactors, liquid-handling robots and closed perfusion loops with fine valves and in-line sensors, an optional 10 nm (0.01 µm) ultra-filtered grade of this medium is available.

  • Total Particulate Exclusion: 0.01 µm filtration removes virtually all particulates down to the nanometer scale.
  • Valve & Sensor Protection: keeps micro-valves, pumps and optical or electrochemical sensors free of deposits.
  • Extended Perfusion Stability: supports long, uninterrupted perfusion runs with fewer line changes.

Inquiry Required: the 0.01 µm grade is made on request — email support@diagnocine.com with your catalog number and volume.

Cancer Biology

Soft-Agar Colony Assays

Mix 1:1 with molten agar or agarose for anchorage-independent growth assays at 1X medium strength.[4]

Soft agarColony formationTransformation
Tissue Engineering

Collagen-Gel & Hydrogel Cultures

Fold in collagen, methylcellulose or hydrogels for gel-contraction studies and 3D constructs.[5]

Collagen gelsHydrogels3D culture
Stem Cell Biology

Semisolid & Stem-Cell Workflows

A double-strength base for methylcellulose and other semisolid cultures used in stem- and progenitor-cell research.

MethylcelluloseProgenitorsOrganoids
Cell Biology

Fibroblasts, HEK 293 & HeLa

Supports primary and established lines for growth, transfection and recombinant-protein work after dilution to 1X.[3]

HEK 293HeLaCOS-7Fibroblasts
Microfluidics

Organ-on-a-Chip & MPS

Microchannel-safe medium for perfused organ-, tissue- and body-on-a-chip platforms.[8,9,10]

OoCToCMPS
Live-Cell Imaging

Microscopy & Optical Sensing

Fewer particles means cleaner fields for colony counting, confocal imaging and on-chip biosensors.

ConfocalColony countingBiosensors
Technical Specifications

Specifications & quality control

Release specifications for DCP-DMEM-QB2X. Lot-specific values are reported on the Certificate of Analysis.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] 9.0 g/L Glucose, [+] Sodium Pyruvate, [+] Phenol Red; [−] L-Glutamine, [−] Sodium Bicarbonate, [−] HEPES
Concentration 2X (dilute 1:1 to 1X)
Appearance Red to Orange
pH 7.4 (± 0.04) USP <791>
Osmolality Measured per lot; reported on CoA USP <785>
D-Glucose 9,000 mg/L (9.0 g/L; 4.5 g/L at 1X)
L-Glutamine Not included
Sodium Pyruvate 220 mg/L
Sodium Bicarbonate Not included
Phenol Red 31.8 mg/L
HEPES Not included
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin < 0.05 EU/mL USP <85> BET
Sterility No bacterial or fungal growth after 14 days of incubation USP <71>
Mycoplasma 0.04 µm barrier filtration USP <63> equiv.
Particulate ≥10 µm Meets limits USP <788> M2
Particulate ≥25 µm Meets limits USP <788> M2
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing std. ISO 13485:2016 ISO
Fill environment ISO Class 5 (Class 100) ISO
Storage, Handling & Logistics
Parameter Specification
Storage temperature 2–8 °C, protected from light
Freeze-thaw Do not freeze
Shelf life 12 months; use before the expiry date on the product label
Shipping condition Ice Pack
CO₂ requirement Depends on the buffer you add; supply sodium bicarbonate before use in a CO₂ incubator
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade High-purity, cell-culture-tested components
Traceability Lot-traceable; CoA per lot
Manufacturing QMS ISO 13485:2016 ISO
Regulatory alignment 21 CFR Part 820 (cGMP) aligned
Production method Micro-batch 2X liquid, quadruple-stage filtered; aseptic fill and finish in Totowa, NJ
Intended use For Research Use Only (RUO)
Note: pH and sodium bicarbonate concentration of the prepared medium are critical factors affecting cell growth. This is also influenced by amount of medium and volume of culture vessel used (surface to volume ratio). For example, in large bottles, such as Roux bottles, pH tends to rise perceptibly as a significant volume of carbon dioxide is released. Therefore, optimal conditions of pH, sodium bicarbonate concentration and surface to volume ratio must be determined for each cell type. We recommend stringent monitoring of pH. If needed, pH can be adjusted by using sterile 1N HCl or 1N NaOH or by bubbling in carbon dioxide. If required, supplements can be added to the medium prior to or after filter sterilization observing sterility precautions.
Formulation

Full composition (mg/L)

DCP-DMEM-QB2X is 2X DMEM with 9.0 g/L glucose, sodium pyruvate and Phenol Red. It does not contain L-glutamine, sodium bicarbonate nor HEPES. Users are advised to review the literature for recommendations regarding medium supplementation and physiological growth requirements specific for different cell lines. All 31 components are listed below at 2X strength; values are per-lot release basis and are halved after 1:1 dilution.

Component CAS Number mg/L
INORGANIC SALTS
Calcium chloride dihydrate 10035-04-8 530.000
Ferric nitrate nonahydrate 7782-61-8 0.200
Magnesium sulphate anhydrous 7487-88-9 195.440
Potassium chloride 7447-40-7 800.000
Sodium chloride 7647-14-5 12800.000
Sodium dihydrogen phosphate anhydrous 7558-80-7 218.000
Component CAS Number mg/L
AMINO ACIDS
Glycine 56-40-6 60.000
L-Arginine hydrochloride 1119-34-2 168.000
L-Cystine dihydrochloride 30925-07-6 125.140
L-Histidine hydrochloride monohydrate 5934-29-2 84.000
L-Isoleucine 73-32-5 210.000
L-Leucine 61-90-5 210.000
L-Lysine hydrochloride 657-27-2 292.000
L-Methionine 63-68-3 60.000
L-Phenylalanine 63-91-2 132.000
L-Serine 56-45-1 84.000
L-Threonine 72-19-5 190.00
L-Tryptophan 73-22-3 32.000
L-Tyrosine disodium salt 69847-45-6 207.580
L-Valine 72-18-4 188.00
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 8.000
D-Ca-Pantothenate 137-08-6 8.000
Folic acid 59-30-3 8.000
Nicotinamide 98-92-0 8.000
Pyridoxine hydrochloride 58-56-0 8.000
Riboflavin 83-88-5 0.800
Thiamine hydrochloride 67-03-8 8.000
i-Inositol 87-89-8 14.400
OTHERS
D-Glucose 50-99-7 9000.000
Phenol Red 34487-61-1 31.800
Sodium pyruvate 113-24-6 220.000
Customization: Custom formulations available — pH, glucose, HEPES, salts, and nutrient composition on request. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Manufactured under an ISO 13485:2016 quality management system; aseptic fill and finish in Totowa, NJ, with every lot released against compendial test methods.

verified

ISO 13485:2016 QMS

Documented, audited quality management system aligned with 21 CFR Part 820 (cGMP).

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm water as the formulation base for low ionic and endotoxin background.

biotech

ISO Class 5 Fill & Finish

Aseptic filling in an ISO Class 5 (Class 100) environment in Totowa, NJ, right after the final 0.04 µm polish.

assignment

Micro-Batch Precision

Small, tightly controlled batches for consistent composition and pH from lot to lot.

Endotoxin — USP <85> BET

Released at < 0.05 EU/mL.

Particulate — USP <788> Method 2

Subvisible particles ≥10 µm and ≥25 µm within compendial limits.

Osmolality — USP <785>

Measured per lot and reported on the CoA.

Documentation / CoA

Appearance, pH 7.4 (± 0.04), 14-day sterility, endotoxin and osmolality per lot.

A lot-specific Certificate of Analysis is available for every lot — request it at support@diagnocine.com.
Product Comparison

How DCP-DMEM-QB2X compares

Same DMEM chemistry, different purity class. Conventional columns describe typical single-pass 0.22 µm-filtered catalog media.

Parameter DCP-DMEM-QB2X (FluxMPS™) Conventional DMEM High Glucose, 1X (0.22 µm filtered) Conventional 2X DMEM (0.22 µm filtered)
Formulation 2X DMEM High Glucose; free of L-Glutamine and sodium bicarbonate 1X DMEM, ready to use Double-strength, for semisolid culture
Final filtration pore size 0.04 µm 0.22 µm 0.22 µm
Number of filtration stages 4 1 1
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin specification < 0.05 EU/mL Often not specified at this level Often not specified at this level
USP <788> particulate compliance check_circle cancel cancel
Ultrapure Type 1 water (18.2 MΩ·cm) check_circle Varies Varies
ISO 13485:2016 manufacturing check_circle Varies Varies
Microfluidic channel compatibility check_circle cancel cancel
Custom formulation check_circle cancel cancel
FAQ

Frequently asked questions

Common questions about using DCP-DMEM-QB2X for semisolid, 3D, microfluidic and conventional culture.

Yes. DCP-DMEM-QB2X is quadruple-stage filtered to a 0.04 µm final pore size, so it carries far fewer subvisible particles than 0.22 µm media — approximately 5× cleaner by particulate count. That lowers the risk of clogging microchannels, valves and membranes in organ-on-a-chip, hydrogel-based 3D and microfluidic perfusion systems.[8,9,10]
The medium is filtered four times: 0.1 µm twice and 0.04 µm twice. A single 0.22 µm pass can let mycoplasma and fine particulates through; the 0.04 µm stages act as a mycoplasma barrier and polish out sub-micron particles before ISO Class 5 aseptic fill.[11]
L-glutamine breaks down in liquid medium over time, releasing ammonia that can stress sensitive cultures; a glutamine-free base lets you add fresh L-glutamine or a stable dipeptide just before use. Our complete 2X formulation carries 1,168 mg/L L-glutamine (584 mg/L at 1X). Leaving sodium bicarbonate out lets the solution or matrix you mix with the 2X carry the buffer, or lets you set your own bicarbonate level for your CO₂ tension. Our complete 2X formulation carries 7,400 mg/L sodium bicarbonate (3,700 mg/L at 1X); pH and sodium bicarbonate concentration are critical to cell growth, so monitor pH closely. Users are advised to review the literature for supplementation and physiological growth requirements specific to each cell type.[1,2]
It depends on the buffer you add. With sodium bicarbonate, use a CO₂-controlled incubator; with a non-volatile buffer such as HEPES, CO₂ is not needed for pH control.
Mix 2X DMEM with an equal volume of a compatible solution or matrix — agar, agarose, methylcellulose, collagen, hydrogels, supplements or treatment solutions — so glucose (4.5 g/L), amino acids, vitamins and salts end up at their 1X concentration.[4,5] Serum, antibiotics and other supplements can be added before or after filter sterilization, observing sterility precautions.
The endotoxin specification is < 0.05 EU/mL, tested by the USP <85> bacterial endotoxins test (BET) and reported on the lot Certificate of Analysis. The medium is made with Ultrapure Type 1 water (18.2 MΩ·cm).
Yes. Each lot ships with a CoA covering appearance (red to orange), pH 7.4 (± 0.04), sterility (no bacterial or fungal growth after 14 days of incubation per USP), endotoxin and osmolality results. Request copies at support@diagnocine.com.
Scientific References

Supporting literature

Peer-reviewed background on DMEM, semisolid and collagen-gel culture, Phenol Red, organ-on-a-chip culture and mycoplasma control.

  1. Dulbecco R, Freeman G. Plaque production by the polyoma virus. Virology. 1959;8(3):396–397. doi:10.1016/0042-6822(59)90043-1
  2. Eagle H. Nutrition needs of mammalian cells in tissue culture. Science. 1955;122(3168):501–504. doi:10.1126/science.122.3168.501
  3. Graham FL, Smiley J, Russell WC, Nairn R. Characteristics of a human cell line transformed by DNA from human adenovirus type 5. J Gen Virol. 1977;36(1):59–72. doi:10.1099/0022-1317-36-1-59
  4. Borowicz S, Van Scoyk M, Avasarala S, et al. The soft agar colony formation assay. J Vis Exp. 2014;(92):e51998. doi:10.3791/51998
  5. Bell E, Ivarsson B, Merrill C. Production of a tissue-like structure by contraction of collagen lattices by human fibroblasts of different proliferative potential in vitro. Proc Natl Acad Sci USA. 1979;76(3):1274–1278. doi:10.1073/pnas.76.3.1274
  6. Morton HJ. A survey of commercially available tissue culture media. In Vitro. 1970;6(2):89–108. doi:10.1007/BF02616111
  7. Berthois Y, Katzenellenbogen JA, Katzenellenbogen BS. Phenol red in tissue culture media is a weak estrogen: implications concerning the study of estrogen-responsive cells in culture. Proc Natl Acad Sci USA. 1986;83(8):2496–2500. doi:10.1073/pnas.83.8.2496
  8. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32(8):760–772. doi:10.1038/nbt.2989
  9. Low LA, Mummery C, Berridge BR, Austin CP, Tagle DA. Organs-on-chips: into the next decade. Nat Rev Drug Discov. 2021;20(5):345–361. doi:10.1038/s41573-020-0079-3
  10. Leung CM, de Haan P, Ronaldson-Bouchard K, et al. A guide to the organ-on-a-chip. Nat Rev Methods Primers. 2022;2:33. doi:10.1038/s43586-022-00118-6
  11. Drexler HG, Uphoff CC. Mycoplasma contamination of cell cultures: incidence, sources, effects, detection, elimination, prevention. Cytotechnology. 2002;39(2):75–90. doi:10.1023/A:1022913015916
  12. Halldorsson S, Lucumi E, Gómez-Sjöberg R, Fleming RMT. Advantages and challenges of microfluidic cell culture in polydimethylsiloxane devices. Biosens Bioelectron. 2015;63:218–231. doi:10.1016/j.bios.2014.07.029

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