FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose: 1X Liquid
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose: 1X Liquid
Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 in an ISO Class 5 fill environment using Ultrapure Type 1 water (18.2 MΩ·cm). Final 0.04 µm polish reduces sub-visible particulate load beyond conventional 0.22 µm filtration.
- Quadruple-stage filtration train (0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm) reaching a 0.04 µm final cut-off, engineered for microfluidic channels and organ-on-a-chip (OoC) systems
- HEPES-supplemented (25 mM) DMEM base retaining standard sodium bicarbonate (3700 mg/L) buffering — supports incubator and bench-top handling
- Glucose removed for precise, user-defined carbon-source control in Warburg-effect and metabolic flux studies; L-glutamine (584 mg/L) and sodium pyruvate (110 mg/L) retained
- Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85>), tested per manufacturing batch
- Manufactured under an ISO 13485:2016 quality management system with aseptic ISO Class 5 (Class 100) fill and finish
- Ultrapure Type 1 water (18.2 MΩ·cm) feedstock supports a low trace-metal, low-organic-carbon formulation
- 4× BME amino acid & vitamin concentrations for extended microphysiological system (MPS) perfusion
- Custom pH, glucose reintroduction, HEPES, salts & nutrients available on request — support@diagnocine.com
- GlucoseNone / Not added
- L-Glutamine584 mg/L ([+])
- Sodium Pyruvate110 mg/L ([+])
- pH (USP <791>)7.4
- Osmolality (USP <785>)310 - 350 mOsm/kg
- Endotoxin (USP <85>)< 0.05 EU/mL
- Filtration0.1µm ×2 + 0.04µm ×2
- Storage2-8°C, protect from light
- Shelf Life12 months from date of manufacture, unopened
- ShippingCold pack (2–8°C)
Engineered where standard media fails
Conventional 0.22 µm filtered media allows mycoplasma (typical diameter 0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and invalidating metabolic assays. FluxMPS™ closes that gap.
Microchannel-safe purity
0.04 µm final filter and USP <788> Method 1 (Light Obscuration Particle Count Test) particulate control support unobstructed flow in narrow microfluidic channels.
Total metabolic control
Precisely defined carbon sources enable Warburg-effect studies, glycolysis inhibition, and ¹³C metabolic tracing.
Ultrapure-grade water
Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) for trace-metal and organic-carbon (TOC) control.
Low background for imaging
Ultra-low particulate baseline supports confocal live-cell imaging, fluorescent biosensors, and automated high-content analysis on chip.
Rich, stable nutrient profile
4× BME amino acid and vitamin concentrations with micro-batch precision — tight lot-to-lot consistency for long-duration MPS perfusion.
Customization on demand
pH, glucose, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.
Quadruple-stage filtration system
A validated four-stage sequential filtration train reaches a final pore size of 0.04 µm — two dedicated prefilter + final-filter pairs run in series for full redundancy.
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1
0.1 µm Prefiltration I
Removes large particulates, cell debris, protein aggregates, bacteria and mycoplasma (typical diameter 0.2–0.3 µm). Protects the first 0.04 µm final filter.
-
2
0.04 µm Final filtration I
Retains fine particulates and sub-micron microaggregates not captured by standard 0.22 µm filtration.
-
3
0.1 µm Prefiltration II
Second dedicated prefilter, protecting the second 0.04 µm final filter cartridge.
-
4
0.04 µm Final filtration II — Polish
Ultimate polishing filter; aseptic fill in a validated ISO Class 5 (Class 100) laminar-flow workstation.
Filtration architecture
Two dedicated prefilter + final-filter pairs run in series, each 0.04 µm final filter protected by its own 0.1 µm prefilter, for redundant particulate and bioburden control down to a 0.04 µm cut-off.
© Diagnocine® — DCP-DMEMH-G1X
Full Composition (mg/L)
Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 33 components across 4 categories. All values are per-lot verified and reported on the Certificate of Analysis (CoA).
INORGANIC SALTS
| Component | CAS Number | mg/L |
|---|---|---|
| Calcium chloride dihydrate | 10035-04-8 | 265.000 |
| Ferric nitrate nonahydrate | 7782-61-8 | 0.100 |
| Magnesium sulfate anhydrous | 7487-88-9 | 97.720 |
| Potassium chloride | 7447-40-7 | 400.000 |
| Sodium bicarbonate | 144-55-8 | 3700.000 |
| Sodium chloride | 7647-14-5 | 6400.000 |
| Sodium dihydrogen phosphate anhydrous | 7558-80-7 | 109.000 |
AMINO ACIDS
| Component | CAS Number | mg/L |
|---|---|---|
| Glycine | 56-40-6 | 30.000 |
| L-Arginine hydrochloride | 1119-34-2 | 84.000 |
| L-Cystine dihydrochloride | 30925-07-6 | 62.570 |
| L-Glutamine | 56-85-9 | 584.000 |
| L-Histidine hydrochloride monohydrate | 5934-29-2 | 42.000 |
| L-Isoleucine | 73-32-5 | 105.000 |
| L-Leucine | 61-90-5 | 105.000 |
| L-Lysine hydrochloride | 657-27-2 | 146.000 |
| L-Methionine | 63-68-3 | 30.000 |
| L-Phenylalanine | 63-91-2 | 66.000 |
| L-Serine | 56-45-1 | 42.000 |
| L-Threonine | 72-19-5 | 95.000 |
| L-Tryptophan | 73-22-3 | 16.000 |
| L-Tyrosine Disodium Salt dihydrate | 69847-45-6 | 103.790 |
| L-Valine | 72-18-4 | 94.000 |
| Component | CAS Number | mg/L |
|---|---|---|
| VITAMINS | ||
| Choline chloride | 67-48-1 | 4.000 |
| D-Ca-Pantothenate | 137-08-6 | 4.000 |
| Folic acid | 59-30-3 | 4.000 |
| Nicotinamide | 98-92-0 | 4.000 |
| Pyridoxal hydrochloride | 65-22-5 | 4.000 |
| Riboflavin | 83-88-5 | 0.400 |
| Thiamine hydrochloride | 67-03-8 | 4.000 |
| i-Inositol | 87-89-8 | 7.200 |
| OTHERS | ||
| Phenol red sodium salt | 34487-61-1 | 15.900 |
| Sodium pyruvate | 113-24-6 | 110.000 |
| HEPES | 7365-45-9 | 5958.000 |
Full technical specification
Every lot of FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose: 1X Liquid is released against comprehensive multi-parameter QC specifications.
| Parameter | Specification |
|---|---|
| Formulation | [+] L-Glutamine, Sodium Bicarbonate, Phenol Red, 25mM HEPES, Calcium, Magnesium, Sodium Pyruvate / [-] Glucose |
| Appearance | Clear solution |
| pH USP <791> | 7.4 |
| Osmolality USP <785> | 310 - 350 mOsm/kg H2O |
| Glucose | None / Not added |
| L-Glutamine | 584 mg/L ([+]) |
| Sodium Pyruvate | 110 mg/L ([+]) |
| Phenol Red | 15.9 mg/L ([+]) |
| Parameter | Specification |
|---|---|
| Endotoxin USP <85> | < 0.05 EU/mL (batch release; see §Quality Assurance) |
| Sterility USP <71> | No growth after 14 days |
| Mycoplasma | 0.1 µm mycoplasma-retentive filtration (not tested per lot) |
| Particulate control USP <788> Method 1 | Light Obscuration Particle Count Test applied per lot |
| Water purity | Ultrapure Type 1, 18.2 MΩ·cm |
| Manufacturing | ISO 13485:2016 ISO |
| Fill environment | ISO Class 5 (Class 100) |
| Parameter | Specification |
|---|---|
| Storage temperature | 2-8°C, protect from light |
| Freeze–thaw | Do not freeze |
| Shelf life | 12 months from date of manufacture, unopened |
| Shipping condition | Cold pack (2–8°C) |
| CO2 requirement | ~10% CO2 recommended (44 mM NaHCO3 formulated for pH 7.4 at ~10% CO2); 25 mM HEPES provides supplemental buffering |
| Parameter | Specification |
|---|---|
| Raw material grade | Pharmaceutical/research grade CoA |
| Manufacturing QMS | ISO 13485:2016 ISO |
| UNSPSC | 41116155 — Molecular biology and cell culture growth media (UNv260801) |
| Regulatory alignment | 21 CFR Part 820 (QMSR) aligned |
| Production method | Micro-batch; Totowa, NJ, USA |
| Intended use | RUO only |
Optimized for next-generation cell biology platforms
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.
Automated Bioreactors & Robotics
For fully automated bioreactor and robotic liquid-handling platforms, an optional 0.01 µm (10 nm) MPS Grade ultra-filtered variant of this formulation is available — see the Grade note in the Purity Architecture section above.
- Total Particulate Exclusion — ultra-fine filtration for sensitive valve and sensor networks
- Valve & Sensor Protection — minimizes fouling in automated microfluidic hardware
- Extended Perfusion Stability — supports long-duration unattended runs
Inquiry Required: the 0.01 µm MPS Grade variant is available on request — contact support@diagnocine.com.
Micro Physiological System (MPS) & Chip
Ultra-low particulate, filtration-controlled media for perfusion in organ chips, tissue chips (ToC), and body-on-a-chip (BoC) devices.
Warburg Effect & Metabolic Research
Glucose-free formulation for Warburg-effect studies, aerobic glycolysis, and cancer metabolomics with user-defined carbon source.
iPSC-Derived Models
Ultra-clean, low-endotoxin baseline minimizes non-specific signals in iPSC differentiation and functional organoid readouts.
Endothelial & Primary Cells
Particle-controlled perfusion media for TEER measurement, endothelial monolayer integrity, and primary cell culture.
Metabolic Flux Analysis
Chemically defined, glucose-free formulation for ¹³C metabolic tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.
Microscopy & Optical Sensing
Low particulate baseline supports confocal microscopy, fluorescent biosensors, and automated imaging on chip.
Manufacturing & compliance
Every batch subjected to multi-parameter lot-release testing before distribution.
ISO 13485:2016 QMS
Manufactured by ISO 13485-certified suppliers. Final packaging, QA and testing at DiagnoCine R&D Center; customization at DiagnoCine Precision, Totowa, NJ, USA.
Ultrapure Type 1 Water
18.2 MΩ·cm feedstock with trace-metal and organic-carbon (TOC) control, supporting low-background performance in biosensor and TEER systems.
ISO Class 5 Fill & Finish
Validated ISO Class 5 laminar-flow workstation with real-time particle monitoring. Preserves all filtration gains in the final container.
Micro-Batch Precision
Small-batch production with tight osmolality and pH control, critical for reproducible long-duration MPS experiments.
Endotoxin — USP <85> BET
LAL assay; assay sensitivity 0.005 EU/mL. Release specification: < 0.05 EU/mL, tested per batch.
Particulate — USP <788> Method 1
Light Obscuration Particle Count Test applied per lot following quadruple-stage 0.04 µm filtration.
Osmolality — USP <785>
Freezing-point depression osmometry. Release range: 310 - 350 mOsm/kg H2O.
Certificate of Analysis (CoA)
Full CoA per lot. Request at support@diagnocine.com with lot number.
- Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
- pH, osmolality, conductivity, appearance and clarity
- Sterility
How DCP-DMEMH-G1X compares
Critical advantages in filtration, mycoplasma control, water quality, and QC depth versus conventional media for MPS and microfluidic applications.
| Parameter | DCP-DMEMH-G1X (FluxMPS™) | Standard DMEM (0.22 µm) | Competitor DMEM (0.22 µm) |
|---|---|---|---|
| Grade | Microfluidics Suitable | Not applicable | Not applicable |
| Formulation definition | [+] L-Glutamine, Sodium Bicarbonate, Phenol Red, 25mM HEPES, Calcium, Magnesium, Sodium Pyruvate / [-] Glucose | Standard | Standard |
| Final filtration pore size | 0.04 µm (40 nm) | 0.22 µm | 0.22 µm |
| Filtration stages | 4 (Quadruple-stage) | 1 | 1 |
| Mycoplasma-retentive filtration | check_circle | cancel | cancel |
| Endotoxin (release specification) | < 0.05 EU/mL | Corning classical liquid media — < 0.25 EU/mL Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL Gibco classical DMEM — Not specified (recorded per lot) |
|
| USP <788> Method 1 particulate compliance | check_circle | cancel | cancel |
| Water quality | Ultrapure Type 1, 18.2 MΩ·cm | Unspecified | Unspecified |
| Manufacturing QMS | ISO 13485:2016 | Varies | Varies |
| Microfluidic channel compatibility | check_circle | cancel | cancel |
| Custom formulation | check_circle On request | cancel | cancel |
Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".
Frequently asked questions
Common questions about FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25mM HEPES w/o Glucose: 1X Liquid (DCP-DMEMH-G1X).
Supporting literature
- Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
- Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
- Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
- Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
- van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
- Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
- Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
- Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x
