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FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25 mM HEPES w/o Glucose, L-Glutamine: 1X Liquid

Product#: DCP-DMEMH-GQ1X
$44.00
DCP-DMEMH-GQ1X
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warning For Research Use Only (RUO). Not intended for clinical, diagnostic, or therapeutic use in humans.
verified ISO 13485 Certified Manufacturing

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25 mM HEPES w/o Glucose, L-Glutamine: 1X Liquid

Contains Sodium Bicarbonate Contains 25 mM HEPES Contains Phenol Red Contains Calcium Contains Magnesium Contains Sodium Pyruvate Without Glucose Without L-Glutamine

Microfluidics Suitable, quadruple-stage ultra-filtered (0.1 µm ×2 + 0.04 µm ×2) 1X liquid cell culture medium engineered for microfluidic channels, organ-on-a-chip (OoC), and microphysiological systems (MPS). Manufactured under ISO 13485:2016 in an ISO Class 5 (Class 100) fill environment using Ultrapure Type 1 water (18.2 MΩ·cm). Approximately 5× fewer particles ≥10 µm by count than conventional 0.22 µm-filtered media (USP <788> Method 1).

  • Quadruple-stage filtration train — 0.1 µm → 0.04 µm → 0.1 µm → 0.04 µm — for mycoplasma-retentive, sub-micron particulate control
  • Endotoxin release specification: < 0.05 EU/mL (LAL, USP <85> BET), tested per manufacturing batch
  • High-buffering formulation — 3700 mg/L sodium bicarbonate plus 25 mM HEPES for extended pH stability
  • Glucose-free and L-glutamine-free formulation (110 mg/L sodium pyruvate retained) for defined metabolic and carbon-source studies
  • Ultrapure Type 1 water (18.2 MΩ·cm) prepared in an ISO Class 5 (Class 100) aseptic fill environment
  • Manufactured under an ISO 13485:2016 quality management system; final QC and packaging at Diagnocine, Totowa, NJ
  • 4× BME amino acid and vitamin concentrations for extended microphysiological system (MPS) perfusion
  • Custom pH, HEPES, salts & nutrient formulations available on request — support@diagnocine.com
DCP-DMEMH-GQ1X · Cell Culture Media UNSPSC: 41116155 | Commodity: Molecular biology and cell culture growth media | (UNv260801)
Dulbecco's Modified Eagle Medium (DMEM), 25 mM HEPES w/o Glucose, L-Glutamine: 1X Liquid
  • GlucoseNot added ([-])
  • L-GlutamineNot added ([-])
  • Sodium Pyruvate110 mg/L ([+])
  • pH (USP <791>)7.4
  • Osmolality (USP <785>)310 - 350 mOsm/kg
  • Endotoxin (USP <85>)< 0.05 EU/mL
  • Filtration0.1µm ×2 + 0.04µm ×2 (Quadruple-stage)
  • Storage2-8°C, protect from light
  • Shelf Life12 months from date of manufacture, unopened
  • ShippingCold pack (2–8°C)
ISO 13485:2016 USP <85> <785> <788> RUO
Why FluxMPS™

Engineered where standard media fails

Conventional 0.22 µm filtered media allows mycoplasma (0.2–0.3 µm), sub-visible particulates, and microaggregates to pass freely — clogging microchannels, corrupting biosensors, and confounding metabolic assays. FluxMPS™ closes that gap.

filter_alt

Microchannel-safe purity

0.04 µm final filter and USP <788> Method 1 particulate testing support unobstructed flow in narrow microfluidic channels.

target

Total metabolic control

Glucose- and L-glutamine-free formulation with 110 mg/L sodium pyruvate retained enables precisely defined carbon-source studies, Warburg-effect research, and ¹³C metabolic tracing.

water_drop

Ultrapure-grade water

Prepared with Ultrapure Type 1 water (18.2 MΩ·cm) under controlled trace-metal and organic-carbon (TOC) limits — supporting sensitive biosensor and electrochemical chip applications.

visibility

Low background for imaging

Ultra-low particulate baseline supports confocal live-cell imaging, fluorescent biosensors, and automated high-content analysis on chip.

science

Rich, stable nutrient profile

4× BME amino acid and vitamin concentrations with micro-batch precision — tight lot-to-lot consistency for long-duration MPS perfusion.

tune

Customization on demand

pH, HEPES, salts, and nutrients adjusted on request. Contact support@diagnocine.com.

Purity Architecture

Quadruple-stage filtration system

A validated four-stage sequential filtration train reaching a final pore size of 0.04 µm — two dedicated prefilter/final-filter pairs run in series, capturing sub-micron particulates and mycoplasma-scale material that 0.22 µm filtration misses.

  1. 1

    0.1 µm Prefiltration I

    Removes large particulates, cell debris, and protein aggregates; protects the first 0.04 µm cartridge.

  2. 2

    0.04 µm Final filtration I

    First 0.04 µm pass; retains sub-micron particulates and microaggregates that pass a 0.22 µm filter, and mycoplasma-scale material (0.2–0.3 µm).

  3. 3

    0.1 µm Prefiltration II

    Second dedicated prefilter, protecting the second 0.04 µm cartridge.

  4. 4

    0.04 µm Final filtration II — Polish

    Ultimate polishing filter; aseptic fill in an ISO Class 5 (Class 100) laminar-flow environment.

Performance vs. conventional media

By USP <788> Method 1 (light obscuration), FluxMPS™ media typically show approximately 5× fewer particles ≥10 µm versus standard 0.22 µm filtered media.

4
Sequential filtration passes (0.1 µm ×2 + 0.04 µm ×2)
0.04
µm Final filter — sub-mycoplasma polishing
Sterility & Mycoplasma: 14-day USP <71> sterility tested per lot. Mycoplasma control is achieved by 0.1 µm mycoplasma-retentive filtration (not tested per lot); mycoplasma organisms typically measure 0.2–0.3 µm in diameter, above this filtration cut-off.
Grade: This product is Microfluidics Suitable, filtered to a 0.04 µm final cut-off. It is not an MPS Grade product — that designation is reserved for the 0.01 µm ultra nano-filtered line, which adds 0.02 µm and 0.01 µm stages after the 0.04 µm polish. For applications requiring the 0.01 µm cut-off, contact support@diagnocine.com.
FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25 mM HEPES w/o Glucose, L-Glutamine: 1X Liquid (DCP-DMEMH-GQ1X) ? Quadruple-stage filtration system (0.1 μm x2 + 0.04 μm x2) for organ-on-a-chip and microfluidic MPS cell culture | Diagnocine
Figure 1. FluxMPS™ Quadruple-stage filtration: 0.1µm Prefiltration I → 0.04µm Final filtration I → 0.1µm Prefiltration II → 0.04µm Final filtration II (Polish) → ISO Class 5 aseptic fill.
© Diagnocine® — DCP-DMEMH-GQ1X
Applications

Optimized for next-generation cell biology platforms

FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25 mM HEPES w/o Glucose, L-Glutamine: 1X Liquid is validated for applications where microchannel cleanliness, signal fidelity, and metabolic precision are critical.

Automated Bioreactors & Robotics

Next-Generation System Uptime

For automated perfusion bioreactors and robotic liquid-handling platforms, Diagnocine offers an optional 0.01 µm (10 nm) ultra nano-filtered MPS Grade variant of this formulation, engineered to further reduce valve fouling and sensor drift in continuous long-duration runs.

  • Total Particulate Exclusion — six-stage cascade down to 0.01 µm
  • Valve & Sensor Protection — minimizes microvalve fouling in automated systems
  • Extended Perfusion Stability — reduced maintenance intervals for long-duration runs

Inquiry Required: The 0.01 µm MPS Grade variant is produced to order. Contact support@diagnocine.com to request this formulation.

Microfluidics

Micro Physiological System (MPS) & Chip

Ultra-low particulate media for perfusion in organ chips, tissue chips, and body-on-a-chip devices.

OoCToCBoCLoCMPS
Cancer Biology

Warburg Effect & Metabolic Research

Glucose-free formulation with defined pyruvate content supports Warburg-effect studies, aerobic glycolysis, and cancer metabolomics.

MCF-7MDA-MB-231HeLaA549
Stem Cell Biology

iPSC-Derived Models

Ultra-clean, low-endotoxin baseline minimizes non-specific signals in iPSC differentiation and functional organoid readouts.

iPSC-NeuronsiPSC-CMiPSC-Hep
Vascular Biology

Endothelial & Primary Cells

Particle-free perfusion media for TEER measurement, endothelial monolayer integrity, and primary cell culture.

HUVECsHAECsPrimary hepatocytes
Metabolomics

Metabolic Flux Analysis

Chemically defined, glucose-free formulation for ¹³C metabolic tracing and NMR metabolomics. Not compatible with Agilent Seahorse XF assays, which require bicarbonate-free, phenol red-free medium.

¹³C tracingNMR
Live-Cell Imaging

Microscopy & Optical Sensing

Ultra-low particulate baseline for confocal microscopy, fluorescent biosensors, and automated imaging on chip.

ConfocalBiosensorsTEER
Technical Specifications

Full technical specification

Every lot of FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25 mM HEPES w/o Glucose, L-Glutamine: 1X Liquid is released against comprehensive multi-parameter QC specifications.

Physical & Chemical Parameters
Parameter Specification
Formulation [+] Sodium Bicarbonate, 25 mM HEPES, Phenol Red, Calcium, Magnesium, Sodium Pyruvate / [-] Glucose, L-Glutamine
Appearance Red to red-orange, clear liquid (phenol red pH indicator present)
pH USP <791> 7.4
Osmolality USP <785> 310 - 350 mOsm/kg H²O
Glucose Not added
L-Glutamine Not added ([-])
Sodium Pyruvate 110 mg/L ([+])
Phenol Red 15.9 mg/L ([+])
Sterility, Purity & Safety Parameters
Parameter Specification
Endotoxin USP <85> BET < 0.05 EU/mL (batch release — see §Manufacturing)
Sterility USP <71> No growth after 14 days
Mycoplasma 0.1 µm mycoplasma-retentive filtration (not tested per lot)
Water purity Ultrapure Type 1, 18.2 MΩ·cm
Manufacturing ISO 13485:2016 ISO
Fill environment ISO Class 5 (Class 100)
Storage, Handling & Logistics
Parameter Specification
Storage 2-8°C, protect from light
Freeze–thaw Do not freeze
Shelf life 12 months from date of manufacture, unopened
Shipping Cold pack (2–8°C)
CO2 requirement HEPES-buffered; reduced CO2 dependence for open/microfluidic culture (validate per cell line). In a CO2 incubator, approximately 9-10% CO2 maintains pH 7.4 given this formulation's 44 mM (3700 mg/L) NaHCO3.
Raw Materials & Regulatory Traceability
Parameter Specification
Raw material grade Pharmaceutical/research grade CoA
Manufacturing QMS ISO 13485:2016 ISO
UNSPSC 41116155 — Molecular biology and cell culture growth media (UNv260801)
Regulatory 21 CFR Part 820 (QMSR) aligned
Production Micro-batch; Totowa, NJ, USA
Intended use RUO only
Formulation

Full composition (mg/L)

Every ingredient below is present in this 1X liquid formulation at the exact concentration listed. Total: 33 components across 4 ingredient categories (Inorganic Salts, Amino Acids, Vitamins, Others), grouped into 3 tabs below. All values are per-lot verified and reported on the Certificate of Analysis (CoA).

INORGANIC SALTS
INORGANIC SALTS
Component CAS Number mg/L
Calcium chloride dihydrate 10035-04-8 265.000
Ferric nitrate nonahydrate 7782-61-8 0.100
Magnesium sulfate anhydrous 7487-88-9 97.720
Potassium chloride 7447-40-7 400.000
Sodium bicarbonate 144-55-8 3700.000
Sodium chloride 7647-14-5 6400.00
Sodium dihydrogen phosphate anhydrous 7558-80-7 109.000
AMINO ACIDS
AMINO ACIDS
Component CAS Number mg/L
Glycine 56-40-6 30.000
L-Arginine hydrochloride 1119-34-2 84.000
L-Cystine dihydrochloride 30925-07-6 62.570
L-Histidine hydrochloride monohydrate 5934-29-2 584.000
L-Isoleucine 73-32-5 42.000
L-Leucine 61-90-5 105.000
L-Lysine hydrochloride 657-27-2 105.000
L-Methionine 63-68-3 146.000
L-Phenylalanine 63-91-2 30.000
L-Phenylalanine 63-91-2 66.000
L-Serine 56-45-1 42.000
L-Threonine 72-19-5 95.000
L-Tryptophan 73-22-3 16.000
L-Tyrosine Disodium Salt dihydrate 69847-45-6 103.790
L-Valine 72-18-4 94.000
Component CAS Number mg/L
VITAMINS
Choline chloride 67-48-1 4.000
D-Ca-Pantothenate 137-08-6 4.000
Folic acid 59-30-3 4.000
Nicotinamide 98-92-0 4.000
Pyridoxal hydrochloride 65-22-5 4.000
Riboflavin 83-88-5 0.400
Thiamine hydrochloride 67-03-8 4.000
i-Inositol 87-89-8 7.200
OTHERS
Phenol red sodium salt 34487-61-1 15.900
Sodium pyruvate 113-24-6 110.000
HEPES 7365-45-9 5958.000
Custom formulations: pH, HEPES, salts, and individual nutrient levels adjustable on request. Contact support@diagnocine.com.
Quality Assurance

Manufacturing & compliance

Every batch is subjected to multi-parameter lot-release testing before distribution.

verified

ISO 13485:2016 QMS

Manufactured by ISO 13485-certified suppliers. Final packaging, QA and testing at Diagnocine R&D Center; customization at Diagnocine Precision, Totowa, NJ, USA.

water_drop

Ultrapure Type 1 Water

18.2 MΩ·cm resistivity, prepared under controlled trace-metal and low-TOC limits.

biotech

ISO Class 5 Fill & Finish

Validated ISO Class 5 laminar-flow workstation with real-time particle monitoring, preserving all filtration gains in the final container.

assignment

Micro-Batch Precision

Small-batch production with tight lot-to-lot QC — critical for reproducible long-duration MPS experiments.

Endotoxin — USP <85> BET

LAL assay; assay sensitivity 0.005 EU/mL. Release specification: < 0.05 EU/mL.

Particulate — USP <788> Method 1

Light obscuration particle count testing performed per lot.

Osmolality — USP <785>

Freezing-point depression osmometry. Release range: 310 - 350 mOsm/kg H²O.

Certificate of Analysis (CoA)

Full CoA per lot. Request at support@diagnocine.com with lot number.

Batch-level quality control. Endotoxin is controlled per manufacturing batch rather than per unit. Every batch is tested before release and must meet the release specification:
  • Endotoxin — LAL assay, USP <85> Bacterial Endotoxins Test; assay sensitivity 0.005 EU/mL; release specification < 0.05 EU/mL
  • pH, osmolality, conductivity, appearance and clarity
  • Sterility
A Certificate of Analysis is available on request at support@diagnocine.com.
Product Comparison

How DCP-DMEMH-GQ1X compares

Critical advantages in filtration, mycoplasma barrier, water quality, and QC depth versus conventional 0.22 µm filtered media.

Parameter DCP-DMEMH-GQ1X (FluxMPS™) Standard DMEM (0.22 µm) Competitor DMEM (0.22 µm)
Grade Microfluidics Suitable Not specified Not specified
Formulation definition [+] Sodium Bicarbonate, 25 mM HEPES, Phenol Red, Calcium, Magnesium, Sodium Pyruvate / [-] Glucose, L-Glutamine Standard Standard
Final filtration pore size 0.04 µm (40 nm) 0.22 µm 0.22 µm
Number of filtration stages 4 (Quadruple) 1 1
Mycoplasma barrier filtration check_circle cancel cancel
Endotoxin (release specification) FluxMPS™ — < 0.05 EU/mL Corning classical liquid media — < 0.25 EU/mL
Sigma-Aldrich DMEM complete medium — ≤ 2 EU/mL
Gibco classical DMEM — Not specified (recorded per lot)
USP <788> particulate compliance check_circle Method 1 tested per lot cancel cancel
Water quality Ultrapure Type 1, 18.2 MΩ·cm Not specified Not specified
Manufacturing QMS ISO 13485:2016 Varies Varies
Microfluidic channel compatibility check_circle cancel cancel
Custom formulation check_circle On request cancel cancel

Comparison figures from published supplier specifications, accessed 2026-09-02. Suppliers that publish no numeric endotoxin specification are shown as "Not specified".

FAQ

Frequently asked questions

Common questions about FluxMPS™ Dulbecco's Modified Eagle Medium (DMEM), 25 mM HEPES w/o Glucose, L-Glutamine: 1X Liquid (DCP-DMEMH-GQ1X).

Yes. DCP-DMEMH-GQ1X is engineered for organ-on-a-chip, microfluidic, and MPS applications. The 0.04 µm final filtration removes sub-micron particulates and mycoplasma-scale material that cause microchannel clogging in standard 0.22 µm media.
Four sequential stages (0.1 µm ×2 + 0.04 µm ×2) retain sub-visible particulates and mycoplasma-scale material (0.2–0.3 µm) that pass conventional 0.22 µm filters, delivering approximately 5× fewer particles ≥10 µm by USP <788> Method 1.
This medium is formulated without glucose to enable precise metabolic control — including Warburg-effect studies, glycolysis inhibition, and ¹³C carbon-source tracing. Add D-glucose at your desired concentration (typically 1–4.5 g/L) or substitute galactose to slow glycolytic flux. Note: this formulation already contains 110 mg/L sodium pyruvate as an alternate carbon/energy source. Contact support@diagnocine.com for pre-supplemented variants.
This formulation is HEPES-buffered (25 mM), giving reduced CO2 dependence for open or microfluidic systems (validate per cell line). If cultured in a CO2 incubator, note that this formulation retains standard-level sodium bicarbonate (44 mM / 3700 mg/L); approximately 9-10% CO2 is required to maintain pH 7.4 under those conditions.
Yes. This is a basal medium compatible with FBS, human serum, growth factors, and other additions. Pre-filter serum-containing and protein-containing additions through a 0.2 µm low-protein-binding PES or PVDF filter before combining — never a 0.04 µm filter, which retains serum proteins and lipoproteins.
Endotoxin is controlled per manufacturing batch. Every batch is tested by LAL assay (USP <85> Bacterial Endotoxins Test, assay sensitivity 0.005 EU/mL) and must meet the release specification of < 0.05 EU/mL before release. Lot-specific results are available on the Certificate of Analysis — request at support@diagnocine.com.
Yes — a full CoA per lot includes: lot number, expiry, appearance, pH (USP <791>), osmolality (USP <785>), endotoxin (USP <85>), sterility (USP <71>), and particulate count (USP <788> Method 1). Email support@diagnocine.com.
Scientific References

Supporting literature

  1. Huh D, et al. Reconstituting organ-level lung functions on a chip. Science. 2010;328:1662–1668. doi:10.1126/science.1188302
  2. Bhatia SN, Ingber DE. Microfluidic organs-on-chips. Nat Biotechnol. 2014;32:760–772. doi:10.1038/nbt.2989
  3. Vander Heiden MG, et al. Understanding the Warburg effect. Science. 2009;324:1029–1033. doi:10.1126/science.1160809
  4. Sontheimer-Phelps A, et al. Modelling cancer in microfluidic human organs-on-chips. Nat Rev Cancer. 2019;19:65–81. doi:10.1038/s41568-018-0104-6
  5. van Duinen V, et al. Microfluidic 3D cell culture. Curr Opin Biotechnol. 2015;35:118–126. doi:10.1016/j.copbio.2015.05.002
  6. Jang KJ, et al. Reproducing human drug toxicities using a Liver-Chip. Sci Transl Med. 2019;11:eaax5516. doi:10.1126/scitranslmed.aax5516
  7. Kasendra M, et al. Primary human Small Intestine-on-a-Chip. Sci Rep. 2018;8:2871. doi:10.1038/s41598-018-21201-7
  8. Skardal A, et al. Multi-tissue organ-on-a-chip platform. Sci Rep. 2017;7:8837. doi:10.1038/s41598-017-08879-x

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