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Dieldrin ELISA Kit MOQ:10

Product#: FNK-CB04-CD01

$99,999,999.00

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Dieldrin ELISA Kit MOQ:10

Cat. No.: FNK-CB04-CD01

Size: 1 kit

Storage: Kit and components shipped at 4°C. Upon receipt, store the kit at 4°C. Stored at the enzyme labeled anti-dieldrin monoclonal antibody at -20°C.

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Description:

The Dieldrin ELISA Kit is an indirect competitive ELISA (ic-ELISA). The assay is designed to detect and quantify the level of dieldrin in soil.

Specifications:

Components

-Competitor coated 96 well stripe well plate (Competitor: Cyclodiene analogue conjugated with protein)
-Peroxidase labeled Anti-dieldrin monoclonal antibody
-Assay buffer
-Wash buffer
-Substrate
-Stop solution:
-Plate sealer

Customer-supplied material

-Standard compound (dieldrin)

Sensitivity: Detection limit; 0.02 ng/mL, Quantification Limit; 0.13 ng/mL.

This data was determined by JIS K0461:2006.

Intra-Assay Precision: 1.6- 4.1 % (n= 3)

Inter-Assay Precision: 3.9- 6.9 % (n= 5)

Recovery (in soil samples): 88- 108 %

For research use only.

Typical standard curve for dieldrin with indirect competitive ELIA (ic-ELISA)

Typical standard curve for dieldrin with indirect competitive ELIA (ic-ELISA)

Cross-reactivity

Chlorinated cyclodienes	Cross-reactivity (%)
dieldrin	100
heptachlor	15
heptachlor-cis-epoxide	21
heptachlor-trans-epoxide	100
aldrin	9
endrin	104
alpha-endosulfan	11
beta-endosulfan	40
endosulfan-sulfate	41
cis-chlordane	18
trans-chlordane	5

% of cross-reactivity was calculated at the 50 % binding point.
Pr.: 30% or lower inhibition by up to 200 ng/mL (40 ng/mL in assay) of chlorinated cyclodienes.

Sample preparation method of soil

Reference

Kataoka, C. et al. Development of a Model Immunoassay Utilizing Monoclonal Antibodies of Different Specificities for Quantitative Determination of Dieldrin and Heptachlors in Their Mixtures. J. Agr. and Food Chem., 2016, 64(46), 8950-8957.

Kataoka, C. et al. Development of a Soil Sample-preparation Method for the Immunoassay of Heptachlors Residues before Cultivation (in Japanese), 2017, BUNSEKI KAGAKU, 66(10), 719-726.