Heptachlor ELISA Kit
Product#: FNK-CB04-CD02
$891.71
Availability:
Ships in 1-2 Weeks
Heptachlor ELISA Kit
Cat. No. FNK-CB04-CD02
Size 1 Kit
Storage 4°C
Description
The Heptachlor ELISA Kit is an indirect competitive ELISA (ic-ELISA). The assay is designed to detect and quantify the level of heptachlor in soil.
Contents and sttorage
- Kit and components shipped at 4°C. Upon receipt, store the kit at 4°C.
- Stored at the enzyme labeled anti-dieldrin monoclonal antibody at -20°C.
Components
- Competitor coated 96 well stripe well plate (Competitor: Cyclodiene analogue conjugated with protein)
- Peroxidase labeled Anti-dieldrin monoclonal antibody
- Assay buffer
- Wash buffer
- Substrate
- Stop solution
- Plate seale
Customer-supplied materialComponents
- Standard compound (heptachlor)
Sensitivity
- Detection limit; 0.03 ng/mL, Quantification Limit; 0.18 ng/mL.
- The data were determined by JIS K0461:2006.
Intra-Assay Precision: 2.8- 3.9 % (n= 3)
Inter-Assay Precision: 2.8- 4.3 % (n= 5)
Typical standard curve for heptachlor with indirect competitive ELIA (ic-ELISA)
Cross-reactivity
% of cross-reactivity was calculated at the 50 % binding point.
Pr.: 30% or lower inhibition by up to 200 ng/mL (40 ng/mL in assay) of chlorinated cyclodienes.
Pr.: 30% or lower inhibition by up to 200 ng/mL (40 ng/mL in assay) of chlorinated cyclodienes.
Sample preparation method of soil
References
- Kataoka, C. et al. Development of a Model Immunoassay Utilizing Monoclonal Antibodies of Different Specificities for Quantitative Determination of Dieldrin and Heptachlors in Their Mixtures. J. Agr. and Food Chem., 2016, 64(46), 8950-8957.
- Kataoka, C. et al. Development of a Soil Sample-preparation Method for the Immunoassay of Heptachlors Residues before Cultivation (in Japanese), 2017, BUNSEKI KAGAKU, 66(10), 719-726.
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