Heptachlor ELISA Kit

Product#: FNK-CB04-CD02
$891.71
Availability:
Ships in 1-2 Weeks

Heptachlor ELISA Kit


Cat. No. FNK-CB04-CD02
Size 1 Kit
Storage 4°C


Description

The Heptachlor ELISA Kit is an indirect competitive ELISA (ic-ELISA). The assay is designed to detect and quantify the level of heptachlor in soil.


Contents and sttorage

  • Kit and components shipped at 4°C. Upon receipt, store the kit at 4°C.
  • Stored at the enzyme labeled anti-dieldrin monoclonal antibody at -20°C.

Components
  • Competitor coated 96 well stripe well plate (Competitor: Cyclodiene analogue conjugated with protein)
  • Peroxidase labeled Anti-dieldrin monoclonal antibody
  • Assay buffer
  • Wash buffer
  • Substrate
  • Stop solution
  • Plate seale

Customer-supplied materialComponents
  • Standard compound (heptachlor)

Sensitivity
  • Detection limit; 0.03 ng/mL, Quantification Limit; 0.18 ng/mL.
  • The data were determined by JIS K0461:2006.

Intra-Assay Precision:  2.8- 3.9 % (n= 3)

Inter-Assay Precision:  2.8- 4.3 % (n= 5)



Typical standard curve for heptachlor with indirect competitive ELIA (ic-ELISA)
 
CB04-CD02-F1.png



Cross-reactivity
CB04-CD02-F2.png
% of cross-reactivity was calculated at the 50 % binding point.
Pr.: 30% or lower inhibition by up to 200 ng/mL (40 ng/mL in assay) of chlorinated cyclodienes.

 

Sample preparation method of soil
CB04-CD01-F3.png


References
  1. Kataoka, C. et al. Development of a Model Immunoassay Utilizing Monoclonal Antibodies of Different Specificities for Quantitative Determination of Dieldrin and Heptachlors in Their Mixtures. J. Agr. and Food Chem., 2016, 64(46), 8950-8957.
  2. Kataoka, C. et al. Development of a Soil Sample-preparation Method for the Immunoassay of Heptachlors Residues before Cultivation (in Japanese), 2017, BUNSEKI KAGAKU, 66(10), 719-726.



 

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